Quantitative determination of proteins at nanogram levels by the resonance light-scattering technique with composite nanoparticles of CdS/PAA

Chen, Hongqi; Xu, Fagong; Hong, Sungwook; Wang, Lun

doi:10.1016/j.saa.2005.10.055

Cited by 22 publications

(5 citation statements)

References 17 publications

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“…It detects RRS signals with a common spectrouorometer and has been widely employed in the designation of bio-assemblies and aggregation species. [19][20][21][22][23] Recent studies have shown RRS is a valuable technique for the analysis of nucleic acids, proteins, and inorganic ions. In recent years, some people have used QDs as potential RRS probes.…”

Section: Introductionmentioning

confidence: 99%

A novel fluorescence and resonance Rayleigh scattering probe based on quantum dots for the detection of albendazole

Qin¹,

Tan

Fu³

et al. 2015

Anal. Methods

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Section: Introductionmentioning

confidence: 99%

A novel fluorescence and resonance Rayleigh scattering probe based on quantum dots for the detection of albendazole

Qin¹,

Tan

Fu³

et al. 2015

Anal. Methods

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“…However, Asja Jhonsi et al discovered that the interaction between uncapped CdS and BSA was very weak [3]. So, a lot of studies have focused on capped CdS nanoparticles by using various capping agents, such as thioglycerol [8], L-cysteine [9], acrylic acid [10], and starch [11]. And the results show that the most sensitive quantitation of protein is generally based on their fluorescence enhancement effect on organic dyes.…”

Section: Introductionmentioning

confidence: 99%

Study on the Interaction between Cadmium Sulphide Nanoparticles and Proteins by Resonance Rayleigh Scattering Spectra

Zhu

Wang

Su³

2013

Journal of Chemistry

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The interaction of cadmium sulphide nanoparticles[(CdS)n]with proteins has been studied by resonance Rayleigh scattering spectra (RRS). Below the isoelectric point, proteins such as bovine serum albumin (BSA), human serum albumin (HSA), lysozyme (Lys), hemoglobin (HGB), and ovalbumin (OVA) can bind withCdSnto form macromolecules by virtue of electrostatic attraction and hydrophobic force. It can result in the enhancement of resonance Rayleigh scattering spectra (RRS) intensity. Their maximum scattering peaks were 280 nm, and there was a smaller peak at 370 nm. The scattering enhancement (ΔIRRS) is directly proportional to the concentration of proteins. A new RRS method for the determination of trace proteins using uncappedCdSnnanoparticles probe has been developed. The detection limits are 19.6 ng/mL for HSA, 16.7 ng/mL for BSA, 18.5 ng/mL for OVA, 80.2 ng/mL for HGB, and 67.4 ng/mL for Lys, separately. In this work, the optimum condition of reaction, the effect of foreign, and the analytical application had been investigated.

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“…[15][16][17][18][19][20] Recently, some immunoreaction, nanogold labeled immunoreaction and immunonanogold catalytic reaction have been utilized to establish some new RS methods for detection of antigen and haptene. [21][22][23][24] In addition, the combining RS technique with inorganic catalytic reaction and enzyme catalytic reaction was applied to analysis of trace inorganic ion, organic compound and enzyme activity.…”

Section: Introductionmentioning

confidence: 99%

A New and Sensitive Catalytic Resonance Scattering Spectral Assay for the Detection of Laccase Activity Using H2O2-I−-TDMAC System

2011

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In pH 3.8 acetic acid-sodium acetate (HAC-NaAC) buffer solution, laccase exhibited a strong catalytic effect on the H 2 O 2 oxidation of I -to form I 2 , and I 2 combined with excess I -to form 3 I -that reacted with cationic surfactants of tetradecyl dimethylbenzyl ammonium chloride (TDMAC) to produce the (TDMAC-I 3 ) n association complex particles, which exhibited a strong resonance scattering (RS) peak at 468 nm. Under the chosen conditions, as the concentration of laccase activity increased, the RS intensity at 468 nm (I 468 nm ) increased linearly. The increased RS intensity ∆I 468 nm was linear to laccase activity in the range of 0.08-0.96 U/mL, with a regression equation of ∆I 468 nm ＝88.8U-1.9, and a detection limit of 0.02 U/mL laccase. This proposed method was applied to detect laccase activity in waste water, with satisfactory results.

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Quantitative determination of proteins at nanogram levels by the resonance light-scattering technique with composite nanoparticles of CdS/PAA

Cited by 22 publications

References 17 publications

A novel fluorescence and resonance Rayleigh scattering probe based on quantum dots for the detection of albendazole

A novel fluorescence and resonance Rayleigh scattering probe based on quantum dots for the detection of albendazole

Study on the Interaction between Cadmium Sulphide Nanoparticles and Proteins by Resonance Rayleigh Scattering Spectra

A New and Sensitive Catalytic Resonance Scattering Spectral Assay for the Detection of Laccase Activity Using H2O2-I−-TDMAC System

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