Quantitative HPLC Analysis of Ascorbic Acid and Gallic Acid in Phyllanthus Emblica

Sawant, Laxman; Prabhakar, Bala; Nancy, P

doi:10.4172/2155-9872.1000111

Cited by 38 publications

(26 citation statements)

References 16 publications

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“…Hence, it is important to mention which guideline has been adopted for the validation, so the method can be easily implemented in other laboratories. Few of the reviewed methods were validated according to official guidelines [31,46,49,54,55,57,63,68,73]. Most of the publications did not mention which guidelines were adopted; instead, the authors used the information provided by some reviews about method validation [2,4,5].…”

Section: Methods Validationmentioning

confidence: 99%

Determination of vitamin C in foods: Current state of method validation

Spínola

Llorent‐Martínez

Castilho

2014

Journal of Chromatography A

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Vitamin C is one of the most important vitamins, so reliable information about its content in foodstuffs is a concern to both consumers and quality control agencies. However, the heterogeneity of food matrixes and the potential degradation of this vitamin during its analysis create enormous challenges. This review addresses the development and validation of high-performance liquid chromatography methods for vitamin C analysis in food commodities, during the period 2000-2014. The main characteristics of vitamin C are mentioned, along with the strategies adopted by most authors during sample preparation (freezing and acidification) to avoid vitamin oxidation. After that, the advantages and handicaps of different analytical methods are discussed. Finally, the main aspects concerning method validation for vitamin C analysis are critically discussed. Parameters such as selectivity, linearity, limit of quantification, and accuracy were studied by most authors. Recovery experiments during accuracy evaluation were in general satisfactory, with usual values between 81 and 109%. However, few methods considered vitamin C stability during the analytical process, and the study of the precision was not always clear or complete. Potential future improvements regarding proper method validation are indicated to conclude this review.

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Section: Methods Validationmentioning

confidence: 99%

Determination of vitamin C in foods: Current state of method validation

Spínola

Llorent‐Martínez

Castilho

2014

Journal of Chromatography A

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“…In this previous study, a standard error of 36.98 was reported for the calibration standard line and is comparable to the current study. Similarly in another study, Sawant et al [30] reported LOD and LOQ values of 1.42 and 4.32 µg/mL respectively for the analysis of ascorbic acid in Phyllanthus Emblica, which were calculated from the calibration standard as was demonstrated in the current study Subsequently, samples diluted to the LOQ level demonstrated good linearity (r 2 =0.991 and 0.972) for the QC and apple extract samples, respectively. Accuracy which was expressed as percent recovery was satisfactory for QC (87 to 103%) and apple extract samples (91 to 99%) for all concentration levels tested.…”

Section: Limit Of Detection and Quantificationmentioning

confidence: 86%

“…Conditions such as pH and organic solvent component contribute to the degree of separation of anayte/s within a sample solution [30]. Two mobile phases were evaluated in an attempt to achieve the best separation and resolution between L-AA and other sample components…”

Section: Mobile Phase Optimizationmentioning

confidence: 99%

Optimization and Validation of a Reverse-Phase High Performance Liquid Chromatography Assay with Ultra-Violet Detection for Measuring Total L-Ascorbic Acid in Food and Beverage Products

Parbhunath¹

2014

J Anal Bioanal Tech

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In accordance with national and international regulatory standards, namely ISO/IEC 17025, the validation of chromatography methods is becoming necessary. This study provides an optimized and fully validated reversephase high performance liquid chromatography (RP-HPLC) assay with ultra-violet (UV) detection for the measurement of L-ascorbic acid (L-AA) in fruit, vegetable and food products.Several commercial fruit juices and teas, fresh fruit and vegetables and food extract products were analyzed using a high performance liquid chromatographic system with UV detection. Chromatographic separation of L-AA was achieved on a reverse phase C 18 150 mm×4.6 mm, 0.5 µm column with UV detection of 245 nm at room temperature. Distilled water/acetonitrile/formic acid (99: 0.9: 0.1, v/v/v) at a flow rate of 1 mLmin -1 was used as the mobile phase, in isocratic mode. Samples were extracted in 4.5% metaphosphoric acid solution and filtered through a 0.45 µm membrane. The method was validated for accuracy, precision, linearity, range, limit of detection, limit of quantification, specificity, stability, robustness and system suitability in accordance with ISO 17025 validation requirements. Validation results demonstrated a linear response within a range of 5 to 125 µg/mL with a correlation coefficient of 0.999 was obtained. Mean recoveries ranged from 99 to 103% and 92 to 96% for L-AA standards and samples, respectively. The method was found to be precise (COV's <5%) and specific with no interferences from coexisting peaks. The LOD and LOQ were 0.61 µg/mL and 1.84 µg/mL respectively.The successful optimization and validation of the proposed method should make it easily applicable for routine laboratory analysis of L-AA measurement in various fruit and vegetable products.

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“…Gooseberry fruits reportedly have a high fraction of hydrolysable tannins such as emblicanin A and B, punigluconin, and pedunculagin among others (Sawant et al, 2012;Dasaroju and Gottumukkala, 2014). Studies have previously reported the presence of alkaloids such as phyllemblin; flavonoids such as kaempferol and phenolic compounds including ellagic acid and gallic acid (Dharmananda, 2003;Habib-ur-Rehman et al, 2007).…”

Section: Mic and Mbcmentioning

confidence: 99%

Evaluation of the antimicrobial activity of Gooseberry and Wild Apple fruit juices of the northeast region of India

Punjabi¹,

Chitalia²,

Mukadam³

et al. 2017

JMHE

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A large number of wild fruits belonging to the north eastern region of India are considered to be nutritionally abundant and traditionally used for the treatment of various ailments. In the present study, juices of gooseberry and wild apple have been evaluated for their antimicrobial activity in its consumable form as a potential source of natural anti-infective agent. The fruit juices were screened for their antibacterial and antifungal activities qualitatively, using the agar well diffusion method followed by quantitative assessment by determining the minimum inhibitory concentration and minimum bactericidal concentration. The study revealed that both the fruit juices exhibited broad spectrum antibacterial activity. Considerable activity against drug-resistant pathogens such as methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, and extended spectrum β-lactamases producing Gram-negative bacteria was observed. Thus, this study highlights the antibacterial efficacy of wild apple and gooseberry fruit juices and augments the beneficiary properties of highly nutritional fruit juices that are specific to Indian region.

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Quantitative HPLC Analysis of Ascorbic Acid and Gallic Acid in Phyllanthus Emblica

Cited by 38 publications

References 16 publications

Determination of vitamin C in foods: Current state of method validation

Determination of vitamin C in foods: Current state of method validation

Optimization and Validation of a Reverse-Phase High Performance Liquid Chromatography Assay with Ultra-Violet Detection for Measuring Total L-Ascorbic Acid in Food and Beverage Products

Evaluation of the antimicrobial activity of Gooseberry and Wild Apple fruit juices of the northeast region of India

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