Quantitative isolation of biocontrol agents Trichoderma spp., Gliocladium spp. and actinomycetes from soil with culture media

Gil, Silvina Vargas; Pastor, Silvina Estela; March, G. J.

doi:10.1016/j.micres.2006.11.022

Cited by 122 publications

(45 citation statements)

References 30 publications

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“…After sterilization in 1% NaOCl for 2 min, the roots were washed with sterile distilled water, weighed, and homogenized using an ULTRA-TURRAX apparatus (Janke & Kunkel) in 20 mL of water for 1 min. Serial dilutions were plated for colony forming unit counts on Trichoderma selective medium (Vargas Gil et al, 2008) at 28°C.…”

Section: Root Colonization Assaymentioning

confidence: 99%

Role of Swollenin, an Expansin-Like Protein fromTrichoderma, in Plant Root Colonization

et al. 2008

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Swollenin, a protein first characterized in the saprophytic fungus Trichoderma reesei, contains an N-terminal carbohydratebinding module family 1 domain (CBD) with cellulose-binding function and a C-terminal expansin-like domain. This protein was identified by liquid chromatography-mass spectrometry among many other cellulolytic proteins secreted in the coculture hydroponics medium of cucumber (Cucumis sativus) seedlings and Trichoderma asperellum, a well-known biocontrol agent and inducer of plant defense responses. The swollenin gene was isolated and its coding region was overexpressed in the same strain under the control of the constitutive pki1 promoter. Trichoderma transformants showed a remarkably increased ability to colonize cucumber roots within 6 h after inoculation. On the other hand, overexpressors of a truncated swollenin sequence bearing a 36-amino acid deletion of the CBD did not differ from the wild type, showing in vivo that this domain is necessary for full protein activity. Root colonization rates were reduced in transformants silenced in swollenin gene expression. A synthetic 36-mer swollenin CBD peptide was shown to be capable of stimulating local defense responses in cucumber roots and leaves and to afford local protection toward Botrytis cinerea and Pseudomonas syringae pv lachrymans infection. This indicates that the CBD domain might be recognized by the plant as a microbe-associated molecular pattern in the Trichoderma-plant interaction.

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Section: Root Colonization Assaymentioning

confidence: 99%

Role of Swollenin, an Expansin-Like Protein fromTrichoderma, in Plant Root Colonization

et al. 2008

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“…After the last disease evaluation, a dilution plate count technique (Vargas Gil et al 2009) was performed, taking 10 g of soil from each pot and diluting them in 90 mL of tap water. After agitation for 90 min at 170 rpm, a serial dilution was made to 1 g L -1 .…”

Section: Methodsmentioning

confidence: 99%

Native Trichoderma grown on oat grains controls damping-off and enhances height in soybean

Roese

Vidal

Zielinski

et al. 2017

Pesqui. Agropecu. Trop.

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Trichoderma is a biological control agent used to improve the resistance to diseases, which may also estimulate plant growth. Commercial products with Trichoderma are available in different countries, but most of them are based on conidial suspension. This study aimed at evaluating the efficiency of native Trichoderma populations collected from different production systems and applied to the soil by using two methods: conidial suspension and inoculated oat grains. The efficiency of native Trichoderma populations collected from conventional crop and agropastoral and agrosilvopastoral systems in a long-term field experiment was evaluated. The populations efficiencies were evaluated by in vivo tests that assessed the control of soybean damping-off caused by Rhizoctonia solani, plant height and soil colonization with the antagonist. In vitro tests, such as dual culture and assessment of volatile and non-volatile compounds, were conducted to study the mode of action of the populations. Some native Trichoderma populations were as efficient as those from a commercial product in all tests. Compared to conidial suspension, Trichoderma spp. inoculated through oat grains promoted a greater damping-off control, higher plants and more colony-forming units per gram of soil after 3 months of application. Native populations performed equally well or even better than the commercial strain, and the use of a substrate that supports the Trichoderma growth was more efficient than the conidial suspension method.

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“…After 48 h of interaction, seedlings were transferred to individual plastic pots (56565 cm) containing sterilized peat moss soil (S-Jord; Hasselfors Garden) and the B. cinerea bioassay was performed following the procedure described previously (Dubey et al, 2013). To recover the T. atroviride strains, plants were carefully removed from the pots, after washing in water the roots were surface sterilized with 1 % NaOCl for 5 min and placed in Rose Bengal medium (Vargas Gil et al, 2009). The bioassay experiment was performed with five biological replicate and each replicate had three plants for each treatment.…”

Section: Construction Of Deletion and Complementation Vectorsmentioning

confidence: 99%

Role of the methylcitrate cycle in growth, antagonism and induction of systemic defence responses in the fungal biocontrol agent Trichoderma atroviride

et al. 2013

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Methylisocitrate lyase (MCL), a signature enzyme of the methylcitrate cycle, which cleaves methylisocitrate to pyruvate and succinate, is required for propionate metabolism, for secondary metabolite production and for virulence in bacteria and fungi. Here we investigate the role of the methylcitrate cycle by generating an mcl deletion mutant in the fungal biocontrol agent Trichoderma atroviride. Gene expression analysis shows that a basal expression of mcl is observed in all growth conditions tested. Phenotypic analysis of an mcl deletion mutant suggests the requirement of MCL in propionate resistance, growth, conidial pigmentation and germination, and abiotic stress tolerance. A plate confrontation assay did not show a difference between the WT and the Dmcl strain in antagonism towards Botrytis cinerea. However, the Dmcl strain displays reduced antagonism towards B. cinerea based on a secretion assay. Furthermore, an in vitro root colonization assay shows that the Dmcl strain had reduced ability to colonize Arabidopsis thaliana roots, which results in reduced induction of systemic resistance towards B. cinerea. These data show that MCL is important not only for growth and development in T. atroviride but also in antagonism, root colonization and induction of defence responses in plants.

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Quantitative isolation of biocontrol agents Trichoderma spp., Gliocladium spp. and actinomycetes from soil with culture media

Cited by 122 publications

References 30 publications

Role of Swollenin, an Expansin-Like Protein fromTrichoderma, in Plant Root Colonization

Role of Swollenin, an Expansin-Like Protein fromTrichoderma, in Plant Root Colonization

Native Trichoderma grown on oat grains controls damping-off and enhances height in soybean

Role of the methylcitrate cycle in growth, antagonism and induction of systemic defence responses in the fungal biocontrol agent Trichoderma atroviride

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