Quantitative Photoelectrochemical Detection of Biotin Conjugated CdSe/ZnS Quantum Dots on the Avidin Immobilized ITO Electrodes

Abstract

Photocurrent generation from CdSe/ZnS (core/shell) quantum dots (QDs) in a photoelectrochemical cell was proposed to perform a bioaffinity biosensor in this study. The photocurrent of QDs is reversible and methylene blue as an electron transfer mediator causes a four-fold increase in the photocurrent. We further present quantitative photoelectrochemical detection of biotin conjugated QDs on the avidin immobilized ITO electrodes. A linear calibration graph was obtained in the range of 4 and 18 nM of biotin conjugated QDs with a coefficient of determination of 0.997. Results imply that QDs can be successfully used as photoelectroactive labels for the photoelectrochemical biosensor systems.

Photoelectrochemical DNA Biosensors

“…The great technological advances in nanoscience have made possible the use of manifold nanomaterials as photoanodes in PEC biosensors. − In particular, inorganic semiconductors, such as SnO 2 , ,− TiO 2 nanoparticles (NPs), − and CdS and CdSe quantum dots (QDs), − have been used extensively in these biosensors due to their excellent PEC properties. The photocurrent generation mechanism of the inorganic semiconductor NPs is shown in Figure .…”

Molybdenum Sulfides and Selenides as Possible Electrocatalysts for CO2 Reduction

“…A variety of techniques have been utilized for detecting sulfhydryl‐containing biomolecules. They include, but are not limited to, spectrofluorometry 8–10, high performance liquid chromatography (HPLC) 11–14, mass spectrometry 15, and photoelectrochemistry 16–20. For spectrofluorometry, a fluorescent probe needs to be synthesized and the probe might experience photobleaching and be subject to background interference.…”

Study of ZnS Nanostructures Based Electrochemical and Photoelectrochemical Biosensors for Uric Acid Detection

“…ZnS nanostructures possessed photoelectrochemical property for its potential application in photoelectric biosensors [21,30]. Electron-hole pairs were yielded in the conduction band and the valence-band with irradiation of light upon ZnS nanostructures.…”

“…Photoelectrochemical method usually has higher sensitivity and low background signals compared with traditional electrochemical method. Several ZnS nanomaterial based photoelectrochemical biosensors have been reported [21,22,23]. However, this is the first time a ZnS based biosensor for UA detection has been presented using the photoelectrochemical method.…”