Quantitative planar chromatography of phospholipids with different fatty acid compositions

Solid phase extraction: Applications to the chromatographic analysis of vegetable oils and fats

“…1978;Gunstone, et al 1994). The fatty acid composition of the various phospholipids depends on the fat origin (Lendrath et al 1991). Fish oils contain similar to mammalian oil phospholipids.…”

Planar Chromatography

“…Densitometric quantification was applied to the following lipid determinations, carried out on unmodified silica gel TLC or HPTLC layers unless otherwise noted: triglyceride composition of tomato, pepper, and grape seed oils by argentation TLC ( 027); neutral lipids in B. glabrata snails fed yolk-lettuce versus lettuce-Tetramin diets ( 028) and in two strains of Helisoma trivolvis snails ( 029) on preadsorbent plates with detection by spraying with PMA reagent; triglycerides in model mixtures by argentation and RP-TLC with visualization by charring and scanning in transmittance and reflection modes ( 030); neutral and polar lipids in berries using petroleum ether-diethyl ether-acetic acid (90:10:1) mobile phase (031); lipids in different layers of porcine epidermis and oral epithelium ( 032) and unsaturated fatty acids by argentation TLC ( 033) with detection by charring; canine sebaceous lipids using automated sample application, triple development with three different solvents, and visualization by sulfuric acid charring (034); lipids from partially hydrolyzed beef tallow and fungal mycelia with detection of zones by immersion of the developed plate in sulfuric acid and heating at 110 °C to induce fluorescence (035); amniotic fluid lipids using automated sample application, double development with chloroform-ethanol-triethylamine-water (15:17:15:4) followed by hexane-diethyl ether (10:1), and detection with copper sulfate-phosphoric acid reagent (036); major phospholipid classes in biological materials at 100-ng levels by use of chloroform-methanol-ethyl acetate-40% methylamine (2:2:2:1) mobile phase and cupric sulfatephosphoric acid detection reagent (037); complex mixtures of phospholipid classes from cell membranes using 2-D TLC and scanning laser densitometry (038); phospholipids in liposomes using cupric sulfate-phosphoric acid reagent ( 039); and phospholipids with different fatty acid compositions from a variety of samples using Dittmer-Lester visualization reagent, which reacts with phosphate groups in the compounds (040). separation and characterization of lipid classes by Iatroscan TLC-FID, and it was found that alumina was superior for low concentrations of neutral lipids and silica gel for polar lipids (041).…”