Quantitative proteomics reveals the effect of protein glycosylation in soybean root under flooding stress

Abstract

Flooding stress has a negative impact on soybean cultivation because it severely impairs growth and development. To understand the flooding responsive mechanism in early stage soybeans, a glycoproteomic technique was used. Two-day-old soybeans were treated with flooding for 2 days and roots were collected. Globally, the accumulation level of glycoproteins, as revealed by cross-reaction with concanavalin A decreased by 2 days of flooding stress. Glycoproteins were enriched from total protein extracts using concanavalin A lectin resin and analyzed using a gel-free proteomic technique. One-hundred eleven and 69 glycoproteins were identified without and with 2 days of flooding stress, respectively. Functional categorization of these identified glycoproteins indicated that the accumulation level of proteins related to protein degradation, cell wall, and glycolysis increased, while stress-related proteins decreased under flooding stress. Also the accumulation level of glycoproteins localized in the secretory pathway decreased under flooding stress. Out of 23 common glycoproteins between control and flooding conditions, peroxidases and glycosyl hydrolases were decreased by 2 days of flooding stress. mRNA expression levels of proteins in the endoplasmic reticulum and N-glycosylation related proteins were downregulated by flooding stress. These results suggest that flooding might negatively affect the process of N-glycosylation of proteins related to stress and protein degradation; however glycoproteins involved in glycolysis are activated.

Gel-free/label-free proteomic analysis of root tip of soybean over time under flooding and drought stresses

“…UGGT was essential for protein folding and served as a molecular chaperone to sense and reglucosylate proteins in order to be rebound by calnexin to ensure proper protein folding [42,43]. In the present study, UGGT was markedly repressed in the root under flooding, a finding that was consistent with the flooding-induced damage of endoplasmic reticulum and protein glycosylation pathways [44,45]. In contrast, UGGT was induced under drought.…”

Velcro-like mannose and slime-like sialic acid interactions guide self-adhesion and aggregation of virus N-glycan shields

“…(42) Environmental stresses also change the N-glycan composition. (43)(44)(45) Establishing guiding rules for how changes in sugar composition alter the interfacial environment will provide a new perspective for interpreting the biological changes occurring in these conditions. Our findings also suggest that the interfacial properties of biological systems will change drastically when the composition of surface sugars change.…”

Impact of Post-Translational Modifications of Crop Proteins under Abiotic Stress

“…Mustafa et al [106] revealed that flooding stress affects the glycosylation level of several proteins in germinating soybeans. Disturbance of the secretory pathway was implicated by the decrease in glycoproteins that were classified in secretory pathways and degradation.…”