Quantitative trait locus mapping of floral and related traits using an F₂ population of Aquilegia

Abstract

The objective of this study was to determine quantitative trait loci (QTL) underlying ten floral and related traits in Aquilegia. The traits assessed were calyx diameter, corolla diameter, petal length, petal blade length, sepal length, sepal width, spur length, spur width, plant height and flower number. These are important traits for ornamental value and reproductive isolation of Aquilegia. QTL analysis of these traits was conducted using single‐marker analysis and composite interval mapping (CIM). We used an F2 population consisting of 148 individuals derived from a cross between the Chinese wild species Aquilegia oxysepala and the cultivar Aquilegia flabellata ‘pumila’. Resulting CIM analysis identified 39 QTLs associated with these traits, which were mapped on seven linkage groups. These QTLs could explain 1.22–53.28% of the phenotypic variance. Thirty‐one QTLs, which explained more than 10% of the phenotypic variation, were classified as major QTLs. Graphical representations of the QTLs on seven linkage groups were made. Our research provides the potential for future molecular assisted selection breeding programmes and the cloning of target genes through fine mapping.

Association mapping of drought tolerance indices in wheat: QTL-rich regions on chromosome 4A

“…Additionally, the SNP iniaGBS44415 was associated with TKW-SSI, TKW-YSI, GY-STI and GY-YSI in 2011. In general, markers are frequently associated with more than one trait (e.g., Contreras-Soto et al, 2017b;Liu et al, 2011;Mora et al, 2015), which could be explained by the linkage between markers or possible pleiotropic effects (Zhu et al, 2014). In the context of genetic improvement, the pleiotropic effects of molecular markers could be used to take advantage of more than one trait of interest.…”

Genomic regions associated with physiological, biochemical and yield-related responses under water deficit in diploid potato at the tuber initiation stage revealed by GWAS

“…Frequently, markers are associated with more than one trait [ 49 , 50 ], which could be explained by the linkage between markers or possible pleiotropic effects [ 51 ]. In this study, we detected a common QTL for TW and Glc content under WD conditions (WDp-9.21 similar to WDp-9.15) that co-localizes with the Glucan/water dikinase ( GWD ) gene, which is known for the control of starch degradation [ 52 ].…”

A role for the Auxin Response Factors ARF6 and ARF8 homologs in petal spur elongation and nectary maturation in Aquilegia

“…As Author Manuscript noted above, it may be that the arf_s phenotype of spurs is so significant in petals because their development is much more dependent on cell elongation. At the same time, it seems likely that in addition to AqARF6/8, other genes contribute to petal spur elongation, which appears to be under polygenic control (Kramer & Hodges, 2010;Zhu et al, 2014).…”