Quantity of Protein Deposited on Hydrogel Contact Lenses and Its Relation to Visible Protein Deposits

Ri, Myers; Dw, Larsen; Tsao, Ming Sen; Castellano, Carmen; Ld, Becherer; Fontana, Federico; Nr, Ghormley; Meier, G.

doi:10.1097/00006324-199110000-00003

Cited by 51 publications

(24 citation statements)

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“…For instance, in the application of hydrogel as intraocular lenses, too high hydrophilicity of hydrogel facilitates the adsorption and deposition of protein and Ca 2þ to the lens materials, which is the major cause for the turbidness even opacity of artificial lenses. 36 Thus, adjusting the degree of hydrophilicity for materials is very useful in practical applications. As seen from the curves of CA versus the wetting time (Fig.…”

Section: Wetting Property Of Pegda Hydrogelsmentioning

confidence: 99%

Controllable properties and microstructure of hydrogels based on crosslinked poly(ethylene glycol) diacrylates with different molecular weights

Zhang

Wang

Song

et al. 2011

J of Applied Polymer Sci

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This work describes a comprehensive study of hydrogels based on polyethylene glycol diacrylates (PEGDAs) with the molecular weight (MW) range of 400-2000. The blends of low-and high-molecular weight PEGDA macromers with different ratios were photopolymerized under visible light irradiation, using a blue light sensitive photoinitiator Irgacure819, at the total polymer concentration of 60 wt %. Swelling ratios, wetting property, elastic moduli, transparency, and the microstructure of the resulting hydrogels were investigated. Among them, equilibrium water contents, hydrophilicity, and mesh size of the hydrogels increased while the elastic moduli decreased when increased the PEGDA MW or the content of higher MW PEGDA in the blends. Most of the hydrogels possessed excellent transparency in visible region. The viability of L929 cells on the surface of hydrogel was also estimated. All the selected hydrogels exhibited a relatively high proliferation rate, which demonstrated this hydrogel system with photoinitiator Irgacure819 had good biocompatibility. These results show the properties of PEGDA hydrogel could be easily adjusted by varying PEGDA MW or the ratios of low-and high-MW macromers in the composites. It could be helpful for the design of proper PEGDA hydrogels in the applications as tissue engineering or drug delivery system.

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Section: Wetting Property Of Pegda Hydrogelsmentioning

confidence: 99%

Controllable properties and microstructure of hydrogels based on crosslinked poly(ethylene glycol) diacrylates with different molecular weights

Zhang

Wang

Song

et al. 2011

J of Applied Polymer Sci

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“…These protein films are comprised of several different proteins, with lysozyme being a major constituent. Numerous methods have been developed to evaluate and measure these deposits, including: Rudko (1,2,3), image analysis (2), protein assays (3, 4-6, 8, 16), microscopy (4,14,15,19), UV spectrophotometry (7,8,16), HPLC (9,10), FTIR-ATR (11,17), SDS-PAGE (12-14, 16, 18), fluorescence (14,15,20), and amino acid analyses (16,17). Visual methods such as Rudko, microscopy, and image analysis offer a semi-quantitative way of evaluating deposits but are limited to surface deposits and, particularly with Rudko, are subjective.…”

Section: Introductionmentioning

confidence: 99%

A novel procedure for the extraction of protein deposits from soft hydrophilic contact lenses for analysis

Keith¹,

Hong²,

Christensen³

1997

Current Eye Research

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“…In the protein study (1), we found that the high water content, ionic matrix (group IV) lenses are most prone to developing protein deposits, a result obtained by other groups using various procedures (2)(3)(4). In the lipid study (5), we found that nonionic polymer matrices and higher water content (group II) lenses were most prone to lipid deposition.…”

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confidence: 72%

Protein-lipid interaction on the surface of a hydrophilic contact lens in vitro

Bontempo

Rapp

1997

Current Eye Research

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Protein adsorption on a group IV lens renders the lens surface less hydrophilic and, thereby, more susceptible to lipid deposition, which in turn increases surface hydrophobicity and inhibits additional protein deposition. For a group II lens, positively charged protein competes with and replaces some of the polar lipids attached to the lens. Thus, the interaction of protein and lipid on a lens surface most prone to a particular contaminant apparently makes it less likely for that contaminant to bind.

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Quantity of Protein Deposited on Hydrogel Contact Lenses and Its Relation to Visible Protein Deposits

Cited by 51 publications

References 0 publications

Controllable properties and microstructure of hydrogels based on crosslinked poly(ethylene glycol) diacrylates with different molecular weights

Controllable properties and microstructure of hydrogels based on crosslinked poly(ethylene glycol) diacrylates with different molecular weights

A novel procedure for the extraction of protein deposits from soft hydrophilic contact lenses for analysis

Protein-lipid interaction on the surface of a hydrophilic contact lens in vitro

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