Quantum dots-labeled strip biosensor for rapid and sensitive detection of microRNA based on target-recycled nonenzymatic amplification strategy

Deng, Huaping; Liu, Qianwen; Wang, Xin; Huang, Ru; Liu, Hongxing; Lin, Qiumei; Zhou, Xiaoming; Xing, Da

doi:10.1016/j.bios.2016.09.043

Cited by 92 publications

(34 citation statements)

References 44 publications

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“…Deng et al. (2017) proved a target‐recycled enzyme‐free CHA strategy by utilizing quantum dots‐labeled strip biosensor for miR‐21 detection . In their work, two DNA hairpins (H1 and H2) stably co‐existed in the absence of target miR‐21.…”

Section: Hcc‐associated Mirna Detection Based On Enzyme‐free Isothermmentioning

confidence: 99%

Application of Isothermal Nucleic Acid Signal Amplification in the Detection of Hepatocellular Carcinoma‐Associated MicroRNA

et al. 2018

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Hepatocellular carcinoma (HCC) is a common type of malignant tumor with high incidence and mortality, and is the third leading cause of cancer‐related death in human. High‐precision detection of tumor markers, such as microRNA (miRNA), is expected to contribute to the early diagnosis of HCC as well as improve the survival rate of HCC patients. In recent years, great effort has been made to develop different methods to detect HCC‐associated miRNA, in which signal amplification strategies are incorporated to realize high sensitivity. Among them, isothermal nucleic acid signal amplification is an emerging signal amplification technology which has the advantages of high amplification efficiency, good reproducibility, and easy operation for real‐time analysis. Herein, we provide an overview of the advances in the application of isothermal nucleic acid signal amplification in HCC‐associated miRNA detection by highlighting several representative works in recent years.

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Section: Hcc‐associated Mirna Detection Based On Enzyme‐free Isothermmentioning

confidence: 99%

Application of Isothermal Nucleic Acid Signal Amplification in the Detection of Hepatocellular Carcinoma‐Associated MicroRNA

et al. 2018

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“…It has recently been demonstrated that a class of small, endogenous and non-coding nucleic acid miRNAs are able to prevent transcription of PTEN mRNA (45). According to the current literature, miRNAs have a high level of stability in conventional, formalin-fixed, paraffin-embedded clinical tissue specimens, which suggests that they may have potential as diagnostic molecular markers; however, mRNAs tend to be more susceptible to environmental impact and are more unstable than miRNAs (46)(47)(48). Furthermore, it has previously been suggested that miRNAs serve important roles in tumorigenesis via their regulation of protein coding genes (49,50).…”

Section: Discussionmentioning

confidence: 99%

Elucidating the mechanism of miRNA-214 in the regulation of gingival carcinoma

Gong

Yang

Tian

2017

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to evaluate the expression levels of microRNA (miRNA)-214 in tumor tissue, blood and saliva of patients with gingival carcinoma, and to investigate the mechanisms underlying the infiltration and invasion of gingival carcinoma. Between January 2013 and March 2015, blood and saliva samples, gingival carcinoma tumor specimens and peritumoral tumor tissues were harvested from 56 patients with gingival carcinoma. Blood and saliva samples were also harvested from 33 control patients without gingival carcinoma. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to detect miRNA-214 and protein tyrosine phosphatase gene (PTEN) mRNA levels. Western blotting and ELISA were performed to detect PTEN protein levels. The results of RT-qPCR demonstrated that the expression of PTEN mRNA in tumor tissues, blood and saliva of patients with gingival carcinoma were significantly decreased compared with that of the control group (P<0.05). These findings were consistent were consistent with the results of PTEN protein expression detected via western blotting and ELISA in these samples (P<0.05). Conversely, the expression levels of miRNA-214 in these samples were significantly increased (P<0.05) in patients with gingival carcinoma compared with the control group. The decreased expression of PTEN may be associated with the expression of miRNA-214. miRNA-214 may regulate infiltration and invasion of gingival carcinoma via PTEN. These results suggest that miRNA-214 may be used as a marker of gingival carcinoma.

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“…Lateral flow devices (strip tests) are useful tools for robust detection of biomarkers at the PoC due to their low-cost, simplicity, portability and specificity. In light of this, numerous groups have developed strip-based biosensors for miRNA detection (Hou et al, 2012;Gao et al, 2014;Deng et al, 2017a). Given most medical diagnoses are made after testing a panel of two or more biomarkers, Zheng et al (2018) developed a multiplexed lateral flow assay for the simultaneous detection of miR-21, miR-155 and miR-210 in human serum samples at concentrations as low as 68, 7, and 17 pM, respectively, within 10 min ( Figure 3A).…”

Section: New and Emerging Mirna Detection Platformsmentioning

confidence: 99%

“…Given most medical diagnoses are made after testing a panel of two or more biomarkers, Zheng et al (2018) developed a multiplexed lateral flow assay for the simultaneous detection of miR-21, miR-155 and miR-210 in human serum samples at concentrations as low as 68, 7, and 17 pM, respectively, within 10 min ( Figure 3A). Deng et al (2017a) sought to overcome the limited sensitivity of previous lateral flow based biosensors by incorporating a target recycling amplification strategy, whereby two sequence specific hairpins are used to amplify the signal without the need for added enzymes. The improved sensing mechanism FIGURE 2 | MicroRNA biomarkers in personalized medicine.…”

Section: New and Emerging Mirna Detection Platformsmentioning

confidence: 99%

MicroRNA Biomarkers for Infectious Diseases: From Basic Research to Biosensing

et al. 2020

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In the pursuit of improved diagnostic tests for infectious diseases, several classes of molecules have been scrutinized as prospective biomarkers. Small (18-22 nucleotide), non-coding RNA transcripts called microRNAs (miRNAs) have emerged as promising candidates with extensive diagnostic potential, due to their role in numerous diseases, previously established methods for quantitation and their stability within biofluids. Despite efforts to identify, characterize and apply miRNA signatures as diagnostic markers in a range of non-infectious diseases, their application in infectious disease has advanced relatively slowly. Here, we outline the benefits that miRNA biomarkers offer to the diagnosis, management, and treatment of infectious diseases. Investigation of these novel biomarkers could advance the use of personalized medicine in infectious disease treatment, which raises important considerations for validating their use as diagnostic or prognostic markers. Finally, we discuss new and emerging miRNA detection platforms, with a focus on rapid, point-of-care testing, to evaluate the benefits and obstacles of miRNA biomarkers for infectious disease.

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Quantum dots-labeled strip biosensor for rapid and sensitive detection of microRNA based on target-recycled nonenzymatic amplification strategy

Cited by 92 publications

References 44 publications

Application of Isothermal Nucleic Acid Signal Amplification in the Detection of Hepatocellular Carcinoma‐Associated MicroRNA

Application of Isothermal Nucleic Acid Signal Amplification in the Detection of Hepatocellular Carcinoma‐Associated MicroRNA

Elucidating the mechanism of miRNA-214 in the regulation of gingival carcinoma

MicroRNA Biomarkers for Infectious Diseases: From Basic Research to Biosensing

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