Quaternized amino poly(glycerol-methacrylate)s for enhanced pDNA delivery

Liang, Zhixiang; Wu, Xinshi; Yang, Ying‐Wei; Cui, Li; Wu, Guolin; Gao, Hui

doi:10.1039/c3py00210a

Cited by 31 publications

(30 citation statements)

References 43 publications

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“…3,4 Thus, it is urgent to develop novel antibacterial agents. During the past decades, cationic polymers, [5][6][7][8][9][10][11][12][13][14][15][16][17][18] 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 3 inorganic nanoparticles [23][24][25][26] have been reported to show promising antibacterial activities.…”

Section: Introductionmentioning

confidence: 99%

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Layer-by-Layer (LBL) Self-Assembled Biohybrid Nanomaterials for Efficient Antibacterial Applications

Long

et al. 2015

ACS Appl. Mater. Interfaces

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123

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Although antibiotics have been widely used in clinical applications to treat pathogenic infections at present, the problem of drug-resistance associated with abuse of antibiotics is becoming a potential threat to human beings. We report a biohybrid nanomaterial consisting of antibiotics, enzyme, polymers, hyaluronic acid (HA), and mesoporous silica nanoparticles (MSNs), which exhibits efficient in vitro and in vivo antibacterial activity with good biocompatibility and negligible hemolytic side effect. Herein, biocompatible layer-by-layer (LBL) coated MSNs are designed and crafted to release encapsulated antibiotics, e.g., amoxicillin (AMO), upon triggering with hyaluronidase, produced by various pathogenic Staphylococcus aureus (S. aureus). The LBL coating process comprises lysozyme (Lys), HA, and 1,2-ethanediamine (EDA)-modified polyglycerol methacrylate (PGMA). The Lys and cationic polymers provided multivalent interactions between MSN-Lys-HA-PGMA and bacterial membrane and accordingly immobilized the nanoparticles to facilitate the synergistic effect of these antibacterial agents. Loading process was characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and X-ray diffraction spectroscopy (XRD). The minimal inhibition concentration (MIC) of MSN-Lys-HA-PGMA treated to antibiotic resistant bacteria is much lower than that of isodose Lys and AMO. Especially, MSN-Lys-HA-PGMA exhibited good inhibition for pathogens in bacteria-infected wounds in vivo. Therefore, this type of new biohybrid nanomaterials showed great potential as novel antibacterial agents.

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Section: Introductionmentioning

confidence: 99%

“…EDA-PGMA was synthesized according to our previous reported procedures. 7,8 Preparation of MSN-COOH. MSNs were synthesized according to previous method.…”

Section: Introductionmentioning

confidence: 99%

Layer-by-Layer (LBL) Self-Assembled Biohybrid Nanomaterials for Efficient Antibacterial Applications

Long

et al. 2015

ACS Appl. Mater. Interfaces

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123

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“…Accordingly, we designed various quaternized amino PGMAs to further improve the transfection efficiency and reduce the cytotoxicity . Firstly, PGMA was modified with fatty amines, including methylethylamine (MEA), diethylamine (DEA) and dipropylamine (DPA) via ring opening reactions to form linear and 8‐arm amino PGMAs (PGOHMAs) and subsequently reacted with methyl iodide (MeI) to form quaternized PGOHMAs (QPGOHMAs).…”

Section: Construction Of Polymer‐based Nanoparticles For Therapeutic mentioning

confidence: 99%

Polymer Nanoassembly as Delivery Systems and Anti‐Bacterial Toolbox: From PGMAs to MSN@PGMAs

Wang

Yang

2017

The Chemical Record

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Researches on cargo delivery systems have received burgeoning attention and advanced rapidly. For synthetic nanodevices, polymer nanoassemblies and their inorganic-organic hybrid materials, especially smart mesoporous silica nanoparticle (MSN)-polymer hybrids (e. g., MSN@PGMAs), have attracted increasing attention in recent years. Their superior characteristics and unique features such as dynamic transition of morphology endow them the ability to efficiently entrap cargo molecules and undergo smart cargo delivery and release in response to various external stimuli. In this Personal Account, we present our recent research progress in the construction of cargo delivery systems based on polymers, poly(glycidyl methacrylate) (PGMA) and its derivatives in particular, ranging from polymer nanoparticles, reverse micelles, to vesicles and reverse vesicles, and their performance in the delivery and controlled release of model molecules and therapeutic agents. Significantly, MSN-PGMA hybrid nanoassemblies (MSN@PGMAs), constructed with the aid of atom transfer radical polymerization, host-guest interactions, or layer-by-layer self-assembly techniques, and their potential bio-related applications and anti-bacterial applications as new nanocarriers are reviewed. Finally, the prospects and challenges of such nanoplatforms are also discussed.

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“…The quaternization reaction of the tertiary amino groups was carried out using MeI. [35,36] For example, L-MEA was diluted with methanol, followed by the addition of MeI. The reaction mixture was stirred for 24 hr at room temperature.…”

Section: Experimental Materialsmentioning

confidence: 99%

Antimicrobial activities of polymeric quaternary ammonium salts from poly(glycidyl methacrylate)s

Liang

Zhu

Yang

et al. 2013

Polymers for Advanced Techs

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Amino poly(glycerol methacrylate)s (PGOHMAs) were synthesized from linear and 8-arm poly(glycidyl methacrylate)s (PGMAs) via ring opening reactions with methylethylamine (MEA), diethylamine, and dipropylamine, respectively, which were further modified by quaternization reaction using methyl iodide to obtain quaternized PGMAs (QPGMAs for short). The products were characterized by Fourier transform infrared spectroscopy, proton nuclear magnetic resonance, gel permeation chromatography, and thermogravimetric analysis. The amination percentage of amino PGOHMAs and the degree of quaternization of QPGMAs were calculated by elemental analysis and X-ray photoelectron spectroscopy, respectively. According to the solubility test results, 8-arm PGOHMA modified with MEA (S8-MEA) is the only water-soluble derivative of amino PGOHMAs and was employed as a positive control for the comparison with QPGMAs. Antimicrobial studies on these PGMA derivatives were carried out by testing the minimum inhibitory concentration and the bacteria inhibitive rate against Escherichia coli and Staphylococcus aureus. The results indicated that QPGMAs possessed higher antimicrobial activity than S8-MEA and exhibited increased antimicrobial activity against both bacteria with an increased degree of quaternization in weak basic conditions. Moreover, the chemical structure of PGMA derivatives and pH value of the assay conditions were found to affect the antimicrobial activity.

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Quaternized amino poly(glycerol-methacrylate)s for enhanced pDNA delivery

Cited by 31 publications

References 43 publications

Layer-by-Layer (LBL) Self-Assembled Biohybrid Nanomaterials for Efficient Antibacterial Applications

Layer-by-Layer (LBL) Self-Assembled Biohybrid Nanomaterials for Efficient Antibacterial Applications

Polymer Nanoassembly as Delivery Systems and Anti‐Bacterial Toolbox: From PGMAs to MSN@PGMAs

Antimicrobial activities of polymeric quaternary ammonium salts from poly(glycidyl methacrylate)s

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