Quercetin Improving Lipid Metabolism by Regulating Lipid Metabolism Pathway of Ileum Mucosa in Broilers

Abstract

This study is aimed at evaluating the regulatory mechanism of quercetin on lipid metabolism in the ileum of broilers to better understand these pathways decreasing abdominal fat. 480 chickens were randomly divided into 4 groups (control, 0.02% quercetin, 0.04% quercetin, and 0.06% quercetin). Breast muscle, thigh muscle, and abdominal fat pad were removed and weighed at 42 d of age. Serum was obtained by centrifuging blood samples from the jugular vein (10 ml) to determine high-density lipoprotein (HDL), total cholesterol (TC), low-density lipoprotein (LDL), triglyceride (TG), leptin, and adiponectin using ELISA. About 5 g of the ileum was harvested and immediately frozen in liquid nitrogen for RNA-seq. Then, the confirmation of RNA-seq results by the Real-Time Quantitative PCR (RT-qPCR) method was evaluated using Pearson’s correlation. Compared with control, abdominal fat percentage was significantly decreased with increasing quercetin supplementation, and the best result was obtained at 0.06% dietary quercetin supplementation (P<0.01). Breast muscle percentage was significantly decreased at 0.02% quercetin (P<0.01), and thigh muscle percentage tended to increase (P=0.078). Meanwhile, 0.04% and 0.06% quercetin significantly decreased TG (P<0.01), TC (P<0.01), and LDL content (P<0.05) in serum. Serum leptin and adiponectin contents were significantly increased by 0.04% and 0.06% dietary quercetin supplementation, compared with the control (P<0.01). Analyses of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database were used to identify differently expressed genes and lipid metabolism pathways. Quercetin decreased abdominal fat percentage through regulating fat digestion and absorption, glycerophospholipid metabolism, AMPK signaling pathway, fatty acid degradation, and cholesterol metabolism.

Explore the Mechanism of Astragalus mongholicus Bunge against Nonalcoholic Fatty Liver Disease Based on Network Pharmacology and Experimental Verification

“…Beyond this, we further explored the mechanism by which quercetin improves lipid metabolism because quercetin alleviates lipid accumulation in HepG2 cells, which was also consistent with literature reports [ 31 , 48 ]. Hepatic lipid deposition is caused by an imbalance between lipogenesis and lipolysis.…”

Effect of Quercetin on Lipids Metabolism Through Modulating the Gut Microbial and AMPK/PPAR Signaling Pathway in Broilers

“…The previous study in our lab found 505 differentially expressed genes of AMPK signal pathway in the quercetin treatment, compared with the control, and the liver was the main metabolic site of lipid in broilers ( Wang et al, 2020 ); 0.04% quercetin supplementation decreased fat content of liver in laying hens ( Zhang L. et al, 2013 ). Therefore, these findings together with the present study confirmed that quercetin regulated fat metabolism, thus reduced abdominal fat deposition in broilers.…”

“…In high-fat diet (HFD) fed Wistar rats, an increase in abundance of the families Coriobacteriaceae and Enterobacteriaceae was reported that may directly alter host physiology ( Lecomte et al, 2015 ). The previous results of transcriptome sequencing showed that adenosine monophosphate activated protein kinase (AMPK) signaling pathway was the main signal pathway of lipid metabolism ( Wang et al, 2020 ). Activated AMPK pathway reduced lipid synthesis by suppressing the expression of downstream targets ( Zhao et al, 2019 ).…”

Quercetin ameliorates chicken quality by activating the PI3K/PKB/AMPK signaling pathway in broilers

“…The previous results of transcriptome sequencing showed that the AMPK signaling pathway is one of the main signaling pathways of lipid metabolism. We previously reported 505 differentially expressed genes of the AMPK signaling pathway we found in the quercetin treatments compared with the control and is the first differentially expressed gene signaling pathway ( 30 ).…”