Quercetin protects neuroblastoma SH-SY5Y cells against oxidative stress by inhibiting expression of Krüppel-like factor 4

Xi, Jian; Zhang, Bin; Luo, Feijun; Liu, Junwen; Yang, Tao

doi:10.1016/j.neulet.2012.08.082

Cited by 21 publications

(13 citation statements)

References 23 publications

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“…Quercetin is another bioactive compounds found in Z. capense that may have contributed to limiting the caspase-3 induction caused by rotenone exposure. A number of studies have reported a neuroprotective effect of quercetin, specifically related to inhibiting apoptosis in SH-SY5Y cells [62,63]. …”

Section: Resultsmentioning

confidence: 99%

In vitro neuroprotective potential of four medicinal plants against rotenone-induced toxicity in SH-SY5Y neuroblastoma cells

Seoposengwe

Tonder

Steenkamp

2013

BMC Complement Altern Med

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BackgroundLannea schweinfurthii, Zanthoxylum capense, Scadoxus puniceus and Crinum bulbispermum are used traditionally to treat neurological disorders. The aim of this study was to evaluate the cytoprotective potential of the four plants, after induction of toxicity using rotenone, in SH-SY5Y neuroblastoma cells.MethodsCytotoxicity of the plant extracts and rotenone was assessed using the sulforhodamine B (SRB) assay. Fluorometry was used to measure intracellular redox state (reactive oxygen species (ROS) and intracellular glutathione content), mitochondrial membrane potential (MMP) and caspase-3 activity, as a marker of apoptotic cell death.ResultsOf the tested plants, the methanol extract of Z. capense was the least cytotoxic; LC50 121.3 ± 6.97 μg/ml, while S. puniceus methanol extract was the most cytotoxic; LC50 20.75 ± 1.47 μg/ml. Rotenone reduced intracellular ROS levels after 24 h exposure. Pre-treating cells with S. puniceus and C. bulbispermum extracts reversed the effects of rotenone on intracellular ROS levels. Rotenone exposure also decreased intracellular glutathione levels, which was counteracted by pre-treatment with any one of the extracts. MMP was reduced by rotenone, which was neutralized by pre-treatment with C. bulbispermum ethyl acetate extract. All extracts inhibited rotenone-induced activation of caspase-3.ConclusionThe studied plants demonstrated anti-apoptotic activity and restored intracellular glutathione content following rotenone treatment, suggesting that they may possess neuroprotective properties.

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Section: Resultsmentioning

confidence: 99%

In vitro neuroprotective potential of four medicinal plants against rotenone-induced toxicity in SH-SY5Y neuroblastoma cells

Seoposengwe

Tonder

Steenkamp

2013

BMC Complement Altern Med

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“…Quercetin, a flavonoid polyphenolic compound found in many natural herbs, has been shown to exert neuroprotective effects in both in vivo and in vitro neurodegenerative models; these effects are probably due to its anti-oxidant and anti-inflammatory effects (Zbarsky et al ., 2005; Ahmad et al ., 2011; Richetti et al ., 2011; Xi et al ., 2012; Kim et al ., 2014). Therefore, quercetin might act either independently or synergistically by regulating multiple signaling pathways to block neuro-inflammation.…”

Section: Discussionmentioning

confidence: 99%

Vaccinium bracteatum Thunb. Exerts Anti-Inflammatory Activity by Inhibiting NF-κB Activation in BV-2 Microglial Cells

Kwon

Shi

et al. 2016

Biomol Ther (Seoul)

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This study was designed to evaluate the pharmacological effects of Vaccinium bracteatum Thunb. methanol extract (VBME) on microglial activation and to identify the underlying mechanisms of action of these effects. The anti-inflammatory properties of VBME were studied using lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. We measured the production of nitric oxide (NO), inducible NO synthase (iNOS), cyclooxygenase (COX)-2, prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) as inflammatory parameters. We also examined the effect of VBME on intracellular reactive oxygen species (ROS) production and the activity of nuclear factor-kappa B p65 (NF-κB p65). VBME significantly inhibited LPS-induced production of NO and PGE2 and LPS-mediated upregulation of iNOS and COX-2 expression in a dose-dependent manner; importantly, VBME was not cytotoxic. VBME also significantly reduced the generation of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6. In addition, VBME significantly dampened intracellular ROS production and suppressed NF-κB p65 translocation by blocking IκB-α phosphorylation and degradation in LPS-stimulated BV2 cells. Our findings indicate that VBME inhibits the production of inflammatory mediators in BV-2 microglial cells by suppressing NF-κB signaling. Thus, VBME may be useful in the treatment of neurodegenerative diseases due to its ability to inhibit inflammatory mediator production in activated BV-2 microglial cells.

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“…Quercetin could reduce KLF 4 expression in human neuroblastoma SH-SY5Y cells, and increase the expression of bcl-2/bax ratio. Furthermore, Quercetin can moderate the apoptosis rate of SH-5YSY cell and reduce caspase-3 enzyme activity ( Xi et al, 2012 ). A recent study investigated the neuroprotective effect of mitogen-activated protein (MAP) kinase 5 (ERK5) against oxidative stress.…”

Section: Role Of Klf 4 In Admentioning

confidence: 99%

The Role of KLF4 in Alzheimer’s Disease

Cheng

Zou

Jin

et al. 2018

Front. Cell. Neurosci.

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Krüppel-like factor 4 (KLF4), a member of the family of zinc-finger transcription factors, is widely expressed in range of tissues that play multiple functions. Emerging evidence suggest KLF4’s critical regulatory effect on the neurophysiological and neuropathological processes of Alzheimer’s disease (AD), indicating that KLF4 might be a potential therapeutic target of neurodegenerative diseases. In this review, we will summarize relevant studies and illuminate the regulatory role of KLF4 in the neuroinflammation, neuronal apoptosis, axon regeneration and iron accumulation to clarify KLF4’s status in the pathogenesis of AD.

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Quercetin protects neuroblastoma SH-SY5Y cells against oxidative stress by inhibiting expression of Krüppel-like factor 4

Cited by 21 publications

References 23 publications

In vitro neuroprotective potential of four medicinal plants against rotenone-induced toxicity in SH-SY5Y neuroblastoma cells

In vitro neuroprotective potential of four medicinal plants against rotenone-induced toxicity in SH-SY5Y neuroblastoma cells

Vaccinium bracteatum Thunb. Exerts Anti-Inflammatory Activity by Inhibiting NF-κB Activation in BV-2 Microglial Cells

The Role of KLF4 in Alzheimer’s Disease

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