Quin's oval and other microbiota in the rumens of molasses-fed sheep

Vicini, J.L.; Brulla, W. J.; Davis, C.L.; Bryant, M. P.

doi:10.1128/aem.53.6.1273-1276.1987

Cited by 23 publications

(8 citation statements)

References 12 publications

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“…An increase in the relative abundance of clostridial cluster IX-compatible fragments was found in both trials with SO plus FO supplementation. Some of these T-RF may match rumen bacteria related to Quinella ovalis (Deng et al, 2007), which is a large oval-shaped bacterium that belongs to the Selenomonas-Megasphaera-Sporomusa group (clostridial cluster IX) (Krumholz et al, 1993) and has been found in the rumen of sheep (Vicini et al, 1987) and steers (Tolosa, 2006) fed diets with molasses, as in the present experiments. Furthermore, FISH results showed increases in the numbers of large oval bacteria of clostridial cluster IX in the samples from animals fed lipid-supplemented diets, especially with both oils together, which seems to reinforce the hypothesis that the favored microorganisms are presumably Quinella-like bacteria.…”

Section: Discussionsupporting

confidence: 58%

Changes in the rumen bacterial community in response to sunflower oil and fish oil supplements in the diet of dairy sheep

Belenguer

Toral

Frutos

et al. 2010

Journal of Dairy Science

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Rumen microbial biohydrogenation of dietary unsaturated fatty acids has a major effect on the process of developing healthier dairy products. This study aimed to investigate in vivo the effect of diet supplementation with sunflower (SO) and fish (FO) oils on the rumen bacterial community in dairy sheep. First, 32 lactating ewes, divided in 8 lots of 4 animals each (2 lots per treatment), were fed a high-concentrate total mixed ration supplemented with 0, 2% SO, 1% FO, or 2% SO plus 1% FO. After 21 d, rumen fluid samples were taken from each lot for DNA extraction and fluorescence in situ hybridization (FISH) analysis. In a second experiment, 5 cannulated ewes were first fed the same TMR, with the exception of a higher forage level, and then changed to the same diet supplemented with 2% SO plus 1% FO. After 0, 3, and 10 d, rumen content samples were taken for DNA extraction and FISH analysis (fluid). Total bacteria and the Butyrivibrio group were studied in microbial DNA by terminal RFLP analysis (T-RFLP), and real-time PCR was used to quantify Butyrivibrio bacteria that produce vaccenic acid or stearic acid. In rumen fluid samples, total bacteria and clostridial clusters IX and XIV were analyzed by FISH. Dietary supplementation with SO plus FO seemed to induce important changes in the total bacteria and Butyrivibrio populations, and a high interindividual variation was observed, and the speed of the effect of the lipid supplementation depended on the individual microbial composition. Analysis by T-RFLP and FISH showed increases in cluster IX bacteria with SO plus FO supplementation, presumably Quinella-like microorganisms. The abundances of vaccenic acid- and stearic acid-producing Butyrivibrio relative to total bacteria, estimated by real time PCR, were low (0.28 and 0.18%, respectively, in rumen fluid, and 0.86 and 0.81% in rumen contents) and only that of SA-producing bacteria seemed to be reduced by diets containing FO, although differences were only significant in lactating ewes. The T-RFLP analysis showed a variable effect of lipid supplementation on different bacteria of the family Lachnospiraceae, which includes the cultured bacteria known to be actively involved in rumen biohydrogenation. These results suggest that the latter bacteria do not play a dominant role in this process, and therefore other as-yet-uncultivated microorganisms might be more relevant.

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Section: Discussionsupporting

confidence: 58%

Changes in the rumen bacterial community in response to sunflower oil and fish oil supplements in the diet of dairy sheep

Belenguer

Toral

Frutos

et al. 2010

Journal of Dairy Science

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“…Traditional enumeration techniques have been used extensively to estimate the abundance of methanogens in the GI tracts of domestic animals and humans. The reported results vary over a wide range: from a few methanogenic cells to more than 10 10 ml −1 or (g dry weight) −1 [27, 30, 57–59]. Assuming that bacterial numbers range between 10 10 to 10 11 ml −1 and Eucarya contribute to approximately 15% of the total ribosome abundance, an archaeal rRNA abundance of 2% corresponds to about 2×10 8 to 2×10 9 cells ml −1 .…”

Section: Resultsmentioning

confidence: 97%

Microbial community structure in gastrointestinal tracts of domestic animals: comparative analyses using rRNA-targeted oligonucleotide probes

Lin

Raskin

Stahl

2006

FEMS Microbiology Ecology

131

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The microbial community structure of the gastrointestinal (GI) tracts of various domestic animals (bovine, ovine, caprine, and swine) was evaluated using oligonucleotide probes targeting the small subunit (SSU) ribosomal RNA (rRNA) of major microbial groups. Bacterial, eukaryotic, and archaeal rRNAs were estimated to account for approximately 60–90%, 3–30% and 0.5–3%, respectively, of the total rRNA present in the GI tracts of most of the animals examined in this study. The abundance of phylogenetically defined groups of methanogens and sulfate‐reducing bacteria (SRB) was also evaluated. Methanobacteriales were the predominant methanogens in the rumen of bovine and caprine, while Methanomicrobiales predominated in the ovine rumen. A dominant archaeal group could not be identified in other sampling locations of the GI tract of these ruminants and in the GI tract of pigs, using the available collection of group‐specific probes. Desulfovibrio species were the most abundant Gram‐negative SRB targeted by available probes in the GI tract of nearly all animals.

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“…While Rowe et al (10) found Methanosarcina sp. as a large population (109/ml), Genthner et al (6) and, very recently, Vicini et al (14) found E. limosum in much higher numbers (108 to 109/ml and 105 to 106/ml, respectively) than Methanosarcina sp. (O and 103 to 104/ml, respectively).…”

mentioning

confidence: 96%