Quince (Cydonia oblonga Miller) peel polyphenols modulate LPS-induced inflammation in human THP-1-derived macrophages through NF-κB, p38MAPK and Akt inhibition

Essafi‐Benkhadir, Khadija; Refai, Amira; Riahi, Ichrak; Fattouch, Sami; Karoui, Hakim; Essafi, Makram

doi:10.1016/j.bbrc.2012.01.003

Cited by 92 publications

(69 citation statements)

References 41 publications

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“…In the current study, using previously described methods (29)(30)(31), PMA and IL-4 were used to induce human monocytic leukemia THP-1 cells to differentiate into TAMs. Differentiation was identified by observing changes to cell morphology and the expression of cell surface markers.…”

Section: Discussionmentioning

confidence: 99%

Effects of autophagy regulation of tumor-associated macrophages on radiosensitivity of colorectal cancer cells

Shao

Zhu

Chen

et al. 2016

Molecular Medicine Reports

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Abstract. Tumor-associated macrophages (TAMs), a major component of the tumor microenvironment, are crucial to the processes of tumor growth, infiltration and metastasis, and contribute to drug resistance. The importance of TAMs in radiation resistance of colorectal cancer remains unclear. To investigate the effects of autophagy regulation of TAMs on the radiosensitivity of colorectal cancer cells, the current study induced TAM formation from THP-1 monocyte cells. Sequential treatment of THP-1 cells with PMA for 72 h and human recombinant interleukin-4 for 24 h was used to stimulate THP-1 differentiation to TAMs. Expression of the cell surface markers CD68, CD204 and CD206, and changes to cell morphology were used to confirm successful differentiation. The TAMs were stimulated to promote or inhibit autophagy during co-culture with LoVo colorectal adenocarcinoma cells. The cells were irradiated, with subsequent measurement of LoVo colony formation and apoptosis. Additionally, the expression of p53, Bcl-2, survivin and Smac proteins was assessed by western blotting. Monodansylcadaverin staining was used to analyze the presence of autophagic vacuoles in TAM, and western blot analysis was used to assess the expression of Beclin-1, LC3B I and II, ATG-3, -5 and -7. The results demonstrated TAM autophagy to be markedly altered by rapamycin and bafilomycin A1 treatment. Following co-culture with TAMs, the colony formation rate and survival fraction of LoVo cells were significantly higher than those in the control group (P<0.05). It was further demonstrated that the regulation of autophagy in TAMs was able to inhibit the colony formation of LoVo colorectal cancer cells. Upregulation of TAM autophagy using rapamycin exhibited more effective inhibition of LoVo colony formation than autophagy downregulation. Notably, apoptosis was significantly increased in LoVo cells when co-cultured with TAMs only, or with rapamycin-mediated autophagy upregulated TAMs, compared with LoVo cells cultured alone (P<0.01). Expression of Bcl-2, survivin and p53 were reduced in LoVo cells co-cultured with TAMs, compared with the control group (P<0.05), whereas Smac expression was increased in the co-culture groups (P<0.01). It was demonstrated that rapamycin-mediated autophagy stimulation in TAMs led to reduced expression levels of survivin and Bcl-2, however, Smac expression was increased. The upregulation of autophagy in TAMs inhibited proliferation and induced apoptosis in colon cancer cells, and altered the expression of radiosensitivity-associated proteins. This data indicated that the radiosensitivity of colorectal cancer cells is associated with autophagy of TAM, and that stimulating TAM autophagy may increase the radiosensitivity of colorectal cancer cells.

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Section: Discussionmentioning

confidence: 99%

Effects of autophagy regulation of tumor-associated macrophages on radiosensitivity of colorectal cancer cells

Shao

Zhu

Chen

et al. 2016

Molecular Medicine Reports

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“…These compounds are generally involved in the response to different types of stress, such as in wound healing by lignifications of damaged surfaces and protection against ultraviolet rays or against aggression by pathogens [6,7]. According to the number of nuclei that may contain phenol and to the structural elements that bind these rings to each other, polyphenols are classified into different groups: phenolic acids, flavonoids, stilbenes and lignin [8].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity and effect of quince pulp extract on the corrosion of C-steel in 1M HCl

et al. 2014

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The inhibition effect of quince pulp extract (QPE) on the adsorption and corrosion of carbon steel in 1M HCl solution was investigated using the weight loss method, potentiodynamic polarization and impedance spectroscopy measurements. At first, the quince extract was characterized, its polyphenolic content was qualitatively and quantitatively determined and its anti-oxidant activity was assessed. The HPLC-DAD-MS showed that the quince extract contained a high quantity of polyphenols with the main compounds being neochlorogenic acid, rutin and chlorogenic acid (7.68, 9.05 and 18.57 mg/g fw, respectively), which represent together about 84 % of total phenolics. The extract exhibited a strong antioxidant potential as it inhibited 87 % of the DPPH Á anionic radical. The polarization measurements indicate that the extract acts as a mixed inhibitor. The values of inhibition efficiency increase with inhibitor concentration reaching a maximum value (88 %) at 5 9 10 -1 g/L. The presence of QPE enhances the values of R t and reduces the C dl values. The adsorption of QPE under consideration on a steel/acidic solution

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“…Published reports confirm the anti-inflammatory activity of Cydonia oblonga Miller, e.g., in Essafi-Benkhadir et al [37] it is shown that aqueous acetone extracts from the quince Cydonia oblonga Miller inhibited high levels of the proinflammatory cytokine TNF-a and the chemokine IL-8, used in LPS-stimulated human THP-1-derived macrophages. Quince extract has been compared to the non-steroidal anti-inflammatory drug (NSAIDs) indomethacin (Table 2).…”

Section: Cyclooxygenase Inhibitionmentioning

confidence: 72%

Interactions of Bioactive Quince (Cydonia oblonga Mill.) Extract with Biomolecules

Strugała¹,

Cyboran-Mikołajczyk²,

Wyspiańska³

et al. 2017

Rec. Nat. Prod.

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Abstract:The study is concerned with biological activity of quince (Q) fruit extract (Cydonia oblonga Mill.) towards the phosphatidylcholine liposome and the natural lipid-protein erythrocyte membrane, as well as will explaining the40 way of interaction of the extract with the membrane. The results showed that Q extract protects lipids against oxidation induced by AAPH compound to a similar extent in two models of membrane. Studies with probes (Laurdan and DPH) located at different depths within the membrane lipid bilayer showed that extract caused an increase of the packing order of the polar heads of lipids and a slight decrease in mobility of the acyl chains. Such results suggest that extract molecules associate with the lipid and lipid-protein membrane and can stop the propagation of free radicals within the bilayer by modifying the membrane fluidity. Furthermore, Q extract resulted in the inhibition of the activity of enzymes (cyclooxygenase-1 and cyclooxygenase-2) probably involved in inflammatory reactions in the body. The experimental results proved that extract components can bind to the main plasma protein -human serum albumin, and the quenching mechanism was suggested as static. The obtained quince-albumin binding constants show that the extract probably can be transported from the circulatory system to reach its target organ.

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Quince (Cydonia oblonga Miller) peel polyphenols modulate LPS-induced inflammation in human THP-1-derived macrophages through NF-κB, p38MAPK and Akt inhibition

Cited by 92 publications

References 41 publications

Effects of autophagy regulation of tumor-associated macrophages on radiosensitivity of colorectal cancer cells

Effects of autophagy regulation of tumor-associated macrophages on radiosensitivity of colorectal cancer cells

Antioxidant activity and effect of quince pulp extract on the corrosion of C-steel in 1M HCl

Interactions of Bioactive Quince (Cydonia oblonga Mill.) Extract with Biomolecules

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