Quinolone resistance-determining region mutations and the plasmid-mediated quinolone resistance gene qnrS played important roles in decreased susceptibility to fluoroquinolones among Shigella isolates in southeast China between 1998 and 2013

Pu, Xiaoying; Pan, Jinren; Zhang, Wei; Zheng, Wei; Wang, Haoqiu; Gu, Yaming

doi:10.1016/j.ijantimicag.2014.12.004

Cited by 13 publications

(13 citation statements)

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“…aac(6′)-Ib-cr and qnrS were the most frequent PMQR determinants detected among S. flexneri . This agrees with previous reports that qnrS and aac(6′)-Ib-cr were the predominant PMQR determinants across two provinces located in Eastern China [ 31 , 32 ]. Additionally, one of the aac(6′)-Ib-cr positive isolates co-harbored the qnrS gene.…”

Section: Discussionsupporting

confidence: 93%

Newest data on fluoroquinolone resistance mechanism of Shigella flexneri isolates in Jiangsu Province of China

Qin

Qian

Fan

et al. 2017

Antimicrob Resist Infect Control

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BackgroundTo determine the prevalence, antimicrobial susceptibility patterns and related presence of mutations in quinolone resistance-determining region (QRDR) genes and plasmid-mediated quinolone resistance (PMQR) among Shigella flexneri isolates obtained from Jiangsu Province, China.MethodsA total of 400 Shigella flexneri clinical isolates collected during 2012–2015 were identified by biochemical and serological methods, and the antimicrobial susceptibility pattern was evaluated using the disc-diffusion method. PCR and DNA sequencing were accomplished to identify mutations in gyrA, gyrB, parC and parE, and the presence of qnrA, qnrB, qnrC, qnrD, qnrS, qepA and aac(6′)-Ib-cr genes were also detected.ResultsOf all the Shigella flexneri, 75.8% were resistant to nalidixic acid, and 37.0% were categorized as norfloxacin resistant. Overall, 75.5% of isolates possessed gyrA mutations (Ser83Leu, Asp87Gly/Asn and His211Tyr), while 84.3% had parC mutations (Ser80Ile, Ala81Pho, Gln91His and Ser129Pro). The most prevalent point mutations in gyrA and parC were Ser83Leu (75.5%, 302/400) and Ser80Ile (74.5%, 298/400), relatively. Besides, the Gln517Arg alternation in gyrB was detected in 13 S. flexneri isolates and no mutations were identified in parE. PMQR determinations of qnrB, qnrS and aac(6′)-Ib-cr were detected among 16 strains (4.0%).ConclusionsThe results presented here show that fluoroquinolone resistance in these clinical isolates result from mutations in chromosome, besides, despite the low prevalence of PMQR determinants in Jiangsu, it is essential to continue surveillance PMQR determinants in this area.

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Section: Discussionsupporting

confidence: 93%

Newest data on fluoroquinolone resistance mechanism of Shigella flexneri isolates in Jiangsu Province of China

Qin

Qian

Fan

et al. 2017

Antimicrob Resist Infect Control

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“…In recent years, several regions in the Eastern provinces of China, such as Henan (34), Zhejiang (35) and Anhui (36), have reported a certain level of ciprofloxacin resistance of S. flexneri, ranging from 21.0% to 25.4%, which was much lower than our data (58.0%). Hence, the frequent occurrence of ciprofloxacin resistance requires a further specific test on whether fluoroquinolones can be applied into the treatment or not.…”

Section: Discussioncontrasting

confidence: 96%

Two novel mutations in parE among Shigella flexneri isolated from Jiangsu Province of China, 2016

et al. 2018

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“…We have also previously shown that once introduced into a new geographical region, into an apparently naive human population, S. sonnei can establish new isolated clonal populations which evolve locally ( Holt et al , 2013 ). Antimicrobial resistance is a key driver of the recent S. sonnei global expansions marked by the acquisition of antimicrobial resistance genes and/or mutations in genes encoding the target proteins for antimicrobials ( Pu et al , 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Introduction and establishment of fluoroquinolone-resistant Shigella sonnei into Bhutan

et al. 2015

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Shigella sonnei is a major contributor to the global burden of diarrhoeal disease, generally associated with dysenteric diarrhoea in developed countries but also emerging in developing countries. The reason for the recent success of S. sonnei is unknown, but is likely catalysed by its ability to acquire resistance against multiple antimicrobials. Between 2011 and 2013, S. sonnei exhibiting resistance to fluoroquinolones, the first-line treatment recommended for shigellosis, emerged in Bhutan. Aiming to reconstruct the introduction and establishment of fluoroquinolone-resistant S. sonnei populations in Bhutan, we performed whole-genome sequencing on 71 S. sonnei samples isolated in Bhutan between 2011 and 2013.We found that these strains represented an expansion of a clade within the previously described lineage III, found specifically in Central Asia. Temporal phylogenetic reconstruction demonstrated that all of the sequenced Bhutanese S. sonnei diverged from a single ancestor that was introduced into Bhutan around 2006. Our data additionally predicted that fluoroquinolone resistance, conferred by mutations in gyrA and parC, arose prior to the introduction of the founder strain into Bhutan. Once established in Bhutan, these S. sonnei had access to a broad gene pool, as indicated by the acquisition of extended-spectrum β-lactamase-encoding plasmids and genes encoding type IV pili. The data presented here outline a model for the introduction and maintenance of fluoroquinolone-resistant S. sonnei in a new setting. Given the current circulation of fluoroquinolone-resistant S. sonnei in Asia, we speculate that this pattern of introduction is being recapitulated across the region and beyond.

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Quinolone resistance-determining region mutations and the plasmid-mediated quinolone resistance gene qnrS played important roles in decreased susceptibility to fluoroquinolones among Shigella isolates in southeast China between 1998 and 2013

Cited by 13 publications

References 4 publications

Newest data on fluoroquinolone resistance mechanism of Shigella flexneri isolates in Jiangsu Province of China

Newest data on fluoroquinolone resistance mechanism of Shigella flexneri isolates in Jiangsu Province of China

Two novel mutations in parE among Shigella flexneri isolated from Jiangsu Province of China, 2016

Introduction and establishment of fluoroquinolone-resistant Shigella sonnei into Bhutan

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