Untitled

Abstract

The concentration detection limit was superior to literature reports for detection of fatty acids. The mass detection limit provided approximately an order of magnitude improvement over conventional fluorescence. The reagent is potentially useful for analysis of carboxyl containing analytes at low concentrations.

Synthesis and Properties of (6,7-Dimethoxy-4-coumaryl)alanine: A Fluorescent Peptide Label

“…1 H and 13 C NMR were recorded in CDCl 3 or d 6 -dimethyl sulfoxide using tetramethylsilane as the internal standard. Infrared spectra were recorded from KCl discs.…”

“…In comparison with extrinsic peptide labels, Dmca also offers greater sensitivity for measurements in neutral aqueous solution, compared to dansyl, ortho-phthalaldehyde, fluorescamine, and NDA derivatives. Fluorescein provides one of the strongest fluorophores; however, due to its large size, the utility for peptide labeling is limited (6).…”

“…A preliminary communication of these studies was presented recently (7). (6,7-Dimethoxy-4-coumaryl)alanine (Dmca)…”

Ultraviolet and Fluorescence Derivatization Reagents for Carboxylic Acids Suitable for High Performance Liquid Chromatography: A Review

“…This highly sensitive fluorescent reagent was recently utilized for derivatization of carboxylic acids (Mukherjee et al, 1995. The reaction mechanism involved in the formation of the ester conjugates was similar to that of Br-MMC and shown in Fig.…”

“…fatty acids, bile acids, and prostaglandins) play important physiological roles in humans. The problems encountered in trace analysis of carboxylic acids by ultra violet (UV) absorbance, fluorescence, electrochemical, chemiluminescence, and gas chromatography-electron capture detection (GC-ECD) have been discussed in detail previously (Davis, 1977;Nicholson, 1978;Mukherjee et al, 1995). The UV absorbance of the carboxyl group (maximum absorption wavelength 204nm, log ~= 1 .…”