Rabbit Facial Nerve Regeneration in Autologous Nerve Grafts After Antecedent Injury

Jg, Spector; P, Lee; Derby, A

doi:10.1097/00005537-200004000-00023

Cited by 22 publications

(20 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In agreement with most authors, this study opted for the analysis of density (number of myelinated axons) in relation to neural area and not axonal area 9,10 .…”

Section: Discussionmentioning

confidence: 69%

Quantitative histological analysis of the mandibular branch of the facial nerve in rats

Costa¹,

Salomone²,

Silva³

et al. 2012

Acta Cir. Bras.

View full text Add to dashboard Cite

PURPOSE: To establish a model to quantitative histological analysis of the mandibular branch of the facial nerve in rats. METHODS: Eleven Wistar rats had their right and left mandibular branches of the facial nerve surgically removed and were sacrificed afterwards. Quantitative histological analysis was performed with: a) partial number of axons; b) partial area of the transversal cut of the nerve (9000μm²); c) partial density. The averages of partial density were obtained. The statistical study was established by Wilcoxon test (p=0.05). RESULTS: In relation to density of axons, comparison between sides shows no statistically significant difference (p=0.248; p=0.533). Mean partial density of distal and proximal samples was, respectively, 0.18 ± 0.02 and 0.19 ± 0.02 axons/μm². Comparison between proximal and distal samples shows no statistically significant difference (p=0.859; p=0.182). CONCLUSION: This study has successfully established a model to histological quantitative analysis of the mandibular branch of the facial nerve in rats.

show abstract

“…In agreement with most authors, this study opted for the analysis of density (number of myelinated axons) in relation to neural area and not axonal area 9,10 .…”

Section: Discussionmentioning

confidence: 69%

Quantitative histological analysis of the mandibular branch of the facial nerve in rats

Costa¹,

Salomone²,

Silva³

et al. 2012

Acta Cir. Bras.

View full text Add to dashboard Cite

show abstract

“…Remyelinization in the demyelinized axons can only begin after the complete removal of this myelin debris. There is an inverse relationship between the amount of myelin debris and neural regeneration [13]. Kuhlmann et al [14] reported that myelin debris avoids the myelinization, and removal of myelin debris by the immunoglobulin accelerates the remyelinization.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of glial growth factor and nerve growth factor on the recovery of traumatic facial paralysis

Yildiz

Karlıdağ

Yalçin

et al. 2011

Eur Arch Otorhinolaryngol

View full text Add to dashboard Cite

The aim of this study was to assess the effects of Glial growth factor (GGF) and nerve growth factor (NGF) on nerve regeneration in facial nerve anastomosis. In this study, approximately a 1-mm segment was resected from the facial nerve and the free ends were anastomosed. All animals underwent the same surgical procedure and 30 rabbits were grouped randomly in three groups. Control group, the group without any medications; NGF group, the group receiving 250 ng/0.1 ml NGF in the epineurium at the site of anastomosis; GBF group, the group receiving 500 ng/0.1 ml GGF in the epineurium at the site of anastomosis. Medications were given at the time of surgery, and at 24 and 48 h postoperatively. After 2 months, the sites of anastomosis were excised and examined using the electron microscope. It was found that the best regeneration was in the group receiving GGF as compared to the control group in terms of nerve regeneration. Schwann cell and glial cell proliferation were found to be significantly higher in the group receiving GGF as compared to the group receiving NGF. Besides, the number of myelin debris, an indicator of degeneration, was significantly lower in the group with GGF as compared to NGF and control groups (p < 0.005). Using GGF and NGF in order to increase regeneration after nerve anastomosis in experimental traumatic facial nerve paralysis may be a hopeful alternative treatment option in the future. However, further studies on human studies are required to support these results.

show abstract

“…22,18,20 The compression injury mechanism used in our study was previously established by Cai et al (1998), 5 a method that creates a grade II injury according to Sunderland's classification (1978). 27 The sectioning and suturing method was avoided due to interferences from possible surgical technique differences and a large number of syncinesias.…”

Section: Discussionmentioning

confidence: 99%

“…26 Histological analysis counting the number of myelinated axons is a widely used method in international literature for rabbits, rats and hamsters, and is considered by most authors as an objective method to study neural regeneration. [16][17][18]28,29 However, some authors define a constant neural area 17,18,28 whereas other authors only compare the total nerve axon number. 16,19 In this study we compared the axon number density compared to the total nerve area and a defined partial neural area.…”

Section: Discussionmentioning

confidence: 99%

“…[6][7][8][9][10][11][12] The facial nerve, however, does not respond to repair like peripheral nerves. [13][14][15][16][17][18][19] It is relevant to specifically study facial nerve regeneration, and not only to transpose peripheral nerve research results to injuries of this cranial nerve. There are no published studies counting the number of facial nerve trunk myelinated neurons in rabbits to assess nerve regeneration.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Regeneração pós-traumática do nervo facial em coelhos

Costa¹,

Silva

Korn

et al. 2006

Rev. Bras. Otorrinolaringol.

View full text Add to dashboard Cite

Post traumatic facial paralysis is a frequent disease. This work studies posttraumatic regeneration of the facial nerve in rabbits. Functional and histological analysis compared injured and normal nerves on opposite sides. The left facial nerve trunk of twenty rabbits were subjectedto compression lesion, and sacrificed after two (subgroup AL), four (BL) and six (CL) weeks. Comparison between groups was made by analysing total and partial densities of myelinated axons. Statistical analysis: Tukey Method (p≤0.05). Results: There was partial functional recovery after two weeks, and complete recovery after five weeks. Qualitative analysis demonstrated a degenerative pattern in the AL group, with an increased tissue inflammatory process. Evident regeneration signs were observed in the BL group, and almost complete regeneration was seen in the CL group. Normal nerves (N) had an average TD of 15705.59 and average PD of 21800.75. The BL group had an average TD of 10818.55 and an average PD of 15340.56. The CL group had an average TD of 13920.36 and an average PD of 16589.15. The BL group had an average TD of N equal to 68.88%, and the CL group had an average TD of N equal to 88,63% (statistically significant). N showed a significant higher PD than injured nerves. However, this was not statistically different between BL and CL subgroups. Nerve DT was a more reliable method than PD in this study.

show abstract

Rabbit Facial Nerve Regeneration in Autologous Nerve Grafts After Antecedent Injury

Cited by 22 publications

References 13 publications

Quantitative histological analysis of the mandibular branch of the facial nerve in rats

Quantitative histological analysis of the mandibular branch of the facial nerve in rats

Efficacy of glial growth factor and nerve growth factor on the recovery of traumatic facial paralysis

Regeneração pós-traumática do nervo facial em coelhos

Contact Info

Product

Resources

About