RAD51AP1 promotes progression of ovarian cancer via TGF‐β/Smad signalling pathway

Zhao, Hongyu; Gao, Yan; Chen, Qi; Li, Jie; Ren, Meng; Zhao, Xiaoting; Yue, Wentao

doi:10.1111/jcmm.15877

Cited by 22 publications

(25 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, suppression of RAD51AP1 by siRNA reduced cell proliferation of ovarian cancer (OvCa) cells in vitro [3]. Confirmatory data and emerging evidence for the oncogenic potential of RAD51AP1 in other cancers is strengthening the relevance of this molecule [1,[5][6][7].…”

Section: Introductionmentioning

confidence: 80%

“…For example, we have shown potential involvement of mTOR signaling in OvCa, whereas Zhao et al have shown a relationship between RAD51AP1 and in transforming growth factor β (TGF-β)/Smad signaling pathway. Using scatter plots, a significant positive correlation between RAD51AP1 with SMAD2-4 and TGFBR1 was observed [5]. This can be of increasing importance in OvCa, given that TGF-β can exert pro-tumorigenic effects, involving epithelial-mesenchymal transition (EMT) [16].…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Differential Expression of RAD51AP1 in Ovarian Cancer: Effects of siRNA In Vitro

Filipe

Katopodis

Chudasama

et al. 2022

JPM

View full text Add to dashboard Cite

Background: DNA double strand breaks can affect genome integrity potentially leading to cancer. RAD51-associated protein 1 (RAD51AP1), an accessory protein to RAD51, is critical for homologous recombination, a key DNA damage response pathway. Emerging studies indicate a novel role for RAD51AP1 in carcinogenesis. Here we provide additional insight into the role of RAD51AP1 in ovarian cancer (OvCa). Methods: Gene expression and patient phenotype data were obtained from TCGA and GTEX project consortia for bioinformatics analysis. Immunohistochemistry of OvCa tissue microarray was undertaken. Functional analyses were performed in a SKOV3 OvCa cell line with down-regulation of RAD51AP1 using siRNA. Results: RAD51AP1 is overexpressed at gene level in primary and recurrent OvCa compared to controls. At protein level, RAD51AP1 was up-regulated in low grade serous tumors compared to high grade OvCa. There was higher expression of RAD51AP1 in OvCa metastatic to lymph nodes compared to primary cancer samples. Gene enrichment analyses identified 12 differentially expressed genes (DEGs) related to OvCa, eight of which are also common in tissue from patients with type 2 diabetes mellitus (T2DM). Conclusions: RAD51AP1 is overexpressed in OvCa, Given the link between OvCa and T2DM, the eight-gene signature shows potential for predictive value.

show abstract

Section: Introductionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 96%

Differential Expression of RAD51AP1 in Ovarian Cancer: Effects of siRNA In Vitro

Filipe

Katopodis

Chudasama

et al. 2022

JPM

View full text Add to dashboard Cite

show abstract

“…CCK-8 assay and plate clone formation assay are used to evaluate cell proliferation. CCK-8 assay is performed according to our previous study ( Zhao et al, 2020 ). After transfecting siRNA for 24 h, A2780 cells are cultured in six-well culture plates (1,000/well) for 7 days.…”

Section: Methodsmentioning

confidence: 99%

“…RNA extraction and RT-qPCR are conducted according to our previous study ( Zhao et al, 2020 ). The primer sequences are listed in Supplementary Table 3 .…”

Section: Methodsmentioning

confidence: 99%

Single-Cell RNA-Sequencing Portraying Functional Diversity and Clinical Implications of IFI6 in Ovarian Cancer

Zhao

Gao

et al. 2021

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Ovarian cancer (OC) is one of the most lethal gynecologic malignancies. Most patients die of metastasis due to a lack of other treatments aimed at improving the prognosis of OC patients. In the present study, we use multiple methods to identify prognostic S1 as the dominant subtype in OC, possessing the most ligand–receptor pairs with other cell types. Based on markers of S1, the consensus clustering algorithm is used to explore the clinical treatment subtype in OC. As a result, we identify two clusters associated with distinct survival and drug response. Notably, IFI6 contributes to the cluster classification and seems to be a vital gene in OC carcinogenesis. Functional enrichment analysis demonstrates that its functions involve G2M and cisplatin resistance, and downregulation of IFI6 suppresses proliferation capabilities and significantly potentiates cisplatin-induced apoptosis of OC cells in vitro. To explore possible mechanisms of IFI6 influencing OC proliferation and cisplatin resistance, GSEA is conducted and shows that IFI6 is positively correlated with the NF-κB pathway, which is validated by RT-qPCR. Significantly, we develop a prognostic model including IFI6, RiskScore, which is an independent prognostic factor and presents encouraging prognostic values. Our findings provide novel insights into elucidating the biology of OC based on single-cell RNA-sequencing. Moreover, this approach is potentially helpful for personalized anti-cancer strategies and predicting outcomes in the setting of OC.

show abstract

“…IGF2BP2 enhances circ0000745 and promotes aggressiveness and stemness in OC via miR-3187-3p/ERBB4/PI3K/AKT axis [ 122 ]. By TCGA analysis, down-regulation of RAD51AP1 (RAD51‐dependent homologous recombination) supressed proliferation, migration and invasion of OC cells and correlated with TGF-β/Smad pathway [ 123 ]. Under platinum and paclitaxel (PT) treatment, SFPQ/p54 nrb /SRSF2 pathway plays a crucial role in OC resistance [ 103 ].…”

Section: Influences Of Rbps On Regulating Oc Cellular Signalling Pathwaysmentioning

confidence: 99%

RNA-binding proteins in ovarian cancer: a novel avenue of their roles in diagnosis and treatment

Guo

et al. 2022

J Transl Med

View full text Add to dashboard Cite

Ovarian cancer (OC), an important cause of cancer-related death in women worldwide, is one of the most malignant cancers and is characterized by a poor prognosis. RNA-binding proteins (RBPs), a class of endogenous proteins that can bind to mRNAs and modify (or even determine) the amount of protein they can generate, have attracted great attention in the context of various diseases, especially cancers. Compelling studies have suggested that RBPs are aberrantly expressed in different cancer tissues and cell types, including OC tissues and cells. More specifically, RBPs can regulate proliferation, apoptosis, invasion, metastasis, tumorigenesis and chemosensitivity and serve as potential therapeutic targets in OC. Herein, we summarize what is currently known about the biogenesis, molecular functions and potential roles of human RBPs in OC and their prospects for application in the clinical treatment of OC.

show abstract

RAD51AP1 promotes progression of ovarian cancer via TGF‐β/Smad signalling pathway

Cited by 22 publications

References 39 publications

Differential Expression of RAD51AP1 in Ovarian Cancer: Effects of siRNA In Vitro

Differential Expression of RAD51AP1 in Ovarian Cancer: Effects of siRNA In Vitro

Single-Cell RNA-Sequencing Portraying Functional Diversity and Clinical Implications of IFI6 in Ovarian Cancer

RNA-binding proteins in ovarian cancer: a novel avenue of their roles in diagnosis and treatment

Contact Info

Product

Resources

About