Radial Growth, Sporulation, and Virulence of Mexican Isolates ofHirsutella citriformis¹againstDiaphorina citri²

Field Trial of Aqueous and Emulsion Preparations of Entomopathogenic Fungi Against the Asian Citrus Psyllid (Hemiptera: Liviidae) in a Lime Orchard in Mexico

“…In the evaluation of 5 H. citriformis Mexican strains as blastospores, we found that they induced low mortality against D. citri. Similar results were obtained previously by Pérez-González et al (2015a). In our bioassays, the average mortality ranged from 31 to 48% (Fig.…”

“…Although the LC values are relatively similar for both fungal species, H. citriformis due to its slow development would be more expensive to use in an extensive way. For example, the conidia of the H. citriformis strains used herein germinated after 48 to 72 h (O. P. G., unpublished data) and growth is reportedly slow (radial growth = 0.083 to 0.114 cm/d) (Meyer et al 2007;Pérez-González et al 2015a). For I. fumosorosea, Vu et al (2009) indicated conidia germination within 12 to 24 h, and according to Cabanillas & Jones (2009), this fungus showed fast radial growth (0.33 cm/d); both traits were at least 3 times greater than in H. citriformis.…”

“…Hence, it is necessary to expand the exploration for highly pathogenic H. citriformis strains that allows re-evaluating the role that this fungus species may have for biological control of D. citri. Variability in pathogenicity among strains of H. citriformis may occur (Orduño-Cruz et al 2015;Pérez-González et al 2015a), as it was reported for other species of entomopathogenic fungi, even in those tested against D. citri (Mena et al 2003;Hoy et al 2010). Thus, this factor may allow identifying more aggressive strains with the potential to cause increased mortality in D. citri while it proves to be harmless to other non-target insects.…”

Insight into Biological Control Potential of Hirsutella citriformis against Asian Citrus Psyllid as a Vector of Citrus Huanglongbing Disease in America

“…H. citriformis was first described in 1920 by Speare, followed by Petch in 1923 [ 9 ], and Main in 1951 [ 10 ]. It commonly infects Hemiptera insect species, and its morphology has been previously reported [ 6 , 7 , 10 , 11 , 12 , 13 , 14 ]. This fungus is classified within the Ascomycota Division, Pezizomycotina subphylum, Sordariomycetes class (Sung et al 2007), Hypocreomycetidae subclass, Hypocreales Order, Ophiocordycipitaceae family, Hirsutella genus, and citriformis Species.…”

Isolasi dan Identifikasi Entomopatogen Hirsutella citriformis (Speare) dan Potensi Miselianya sebagai Sumber Inokulum untuk Pengendalian Wereng Cokelat (Nilaparvata lugens Stål.)

“…Rendahnya tingkat pembentukan sinemata tersebut menunjukkan bahwa tingkat sporulasi H. citriformis pada media biakan sangat rendah. Pertumbuhan miselia yang lambat dan tingkat sporulasi rendah pada media biakan juga dilaporkan oleh beberapa peneliti sebelumnya (Casique-Valdez et al 2011;Meyer et al 2007;Pérez-González et al 2015;Subandiyah et al 2000) (Subandiyah et al 2000). Dengan demikian, secara morfologis dan molekuler terkonfirmasi bahwa spesies Hirsutella isolat Bgr 0716 yang menginfeksi WBC Bogor adalah H. citriformis.…”