Radiation Exposure–Induced Changes in the Immune Cells and Immune Factors of Mice With or Without Primary Lung Tumor

Abstract

Recent studies have demonstrated that radiation activates in situ antitumor immunity and consequently induced a synergistic effect of radiotherapy and immunotherapy. However, studies related to radiation-induced changes in immune system of tumor-bearing mice are limited, which are of great significance to improve the efficacy of radioimmunotherapy. In this study, we first established a primary lung tumor mouse model using urethane. Then part of the right lung of the mouse was exposed to X-ray irradiation with a computed tomography–guided small animal irradiator and the changes of immune cells in both peripheral blood and spleen were determined by flow cytometry. Besides, the levels of both cytokines and immunoglobulins in mouse serum were detected by a protein chip. We found that B lymphocytes increased while CD8+ T lymphocytes reduced significantly. Interleukin-3 (IL-3), IL-6, regulated upon activation, normally T-expressed, and presumably secreted factor (RANTES), and vascular endothelial growth factor (VEGF) were found to be decreased after tumor formation, and the similar results have also been observed with kappa, IgG3, IgE, IgM, and IgG2a. After irradiation, lower concentrations of IgD, kappa, and IgM were found in the serum. Our findings indicate that localized tumor irradiation caused some obvious changes like inhibiting the ability of innate immunity, and these changes may be useful in predicting prognosis.

Gene signature based on B cell predicts clinical outcome of radiotherapy and immunotherapy for patients with lung adenocarcinoma

“…Through Cyclic GMP‐AMP synthase (cGAS)‐stimulator of interferon genes (STING) pathway, RT promoted production of type 1 interferon bridging innate immune response and adaptive immune response 61 . Moreover, Jones and Shuxian et al found that radiation‐induced tumor regression via stimulating immunity activation and increasing B and NK cell infiltration 62,63 . All of these results were consistent with our research.…”

“…61 Moreover, Jones and Shuxian et al found that radiation-induced tumor regression via stimulating immunity activation and increasing B and NK cell infiltration. 62,63 All of these results were consistent with our research. In our study, GO and KEGG analysis revealed that TILBSig was highly associated with positive thymic T cell selection, response to X ray, positive regulation of type 1 interferon production, and regulation of stem cell differentiation.…”

Reversing Radiation-Induced Immunosuppression Using a New Therapeutic Modality

“…To test epo upregulation in a murine model, we used the Nanoligomer bioinformatics tool and synthesis pipeline (Fig.1) and produced 29U.439_EPO. With an existing murine radiation model, 11, 57–64 29U.439_EPO was tested as a radioprotector/radiomitigator agent in a pilot study for reversal of radiation-induced immune dysfunction in lung tissue (Fig.5A). Three groups of 4 female C57BL/6NCrl mice were administered either vehicle negative control (group 1 and group 2) or 3 mg/kg 29U.439_EPO (group 3) via intraperitoneal (IP) injection.…”

lncRNA MIAT/HMGB1 Axis Is Involved in Cisplatin Resistance via Regulating IL6-Mediated Activation of the JAK2/STAT3 Pathway in Nasopharyngeal Carcinoma

“…Cytokines and chemokines have been reported to participate in the progression of tumor growth, invasion, metastasis, and drug resistance. The expressions of cytokines and chemokines including IL6, IL-11, TGF-b, IL-5, IL-10, and IL-1b have significant differences after ionizing radiation (IR) or cisplatin treatment in various tumors ( 8 , 11 , 38 , 39 ). Thus, cytokines or chemokines are designated as potential biomarkers for diagnosis and treatment in cancers.…”