Radical Scavenging Glycoprotein Inhibiting Cyclooxygenase-2 and Thromboxane A₂Synthase fromAloe veraGel

Abstract

An active glycoprotein fraction containing 58 % protein was isolated from Aloe vera gel by precipitation with 55 % ammonium sulfate followed by gel permeation using DEAE-Sephacel A-25, Sepharose 6B and Sephadex G-50 columns in a yield of 3 x 10 -3 %. The glycoprotein fraction showed a single band corresponding to a subunit of verectin at the same position when stained with both Coomassie brilliant blue and periodic acid-Schiff reagents on 18 % SDS-PAGE. The molecular weight (14 kDa) was confirmed by Sephadex G-50 column chromatography. The glycoprotein fraction showed a radical scavenging activity against superoxide anion generated by the xanthine-xanthine oxidase system as well as inhibition of cyclooxygenase-2 and reduction of thromboxane A 2 synthase level in vitro.

Therapeutic and Medicinal Uses of <i>Aloe vera</i>: A Review

“…f. syn. Aloe barbadensis Miller, is most biologically active among 400 species [2][3][4]. According to World Health Organisation, medicinal plants would be the best source for obtaining a variety of drugs [5].…”

Administration of Dried Aloe vera Gel Powder Reduced Body Fat Mass in Diet-Induced Obesity (DIO) Rats

“…For example, polysaccharides of between 400 Da and 5 kDa were reported to exhibit potent antitumor activity in vivo ( 9 ). Yagi et al isolated a glycoprotein from Aloe that inhibited cyclooxygenase and reduced thromboxane synthesis ( 10 ).…”

Putative Prophylaxes Updated of Placenta Extract and Aloe vera as Biogenic Stimulants

“…The molecular weight (14 kDa) was confirmed by Sephadex G-50 column chromatography. The glycoprotein fraction showed a radical scavenging activity against superoxide anion generated by the xanthin-xanthine oxidase system as well as inhibition of cyclooxygenase-2 and reduction of thromboxane A2 synthase level in vitro [51] .…”