An active glycoprotein fraction containing 58 % protein was isolated from Aloe vera gel by precipitation with 55 % ammonium sulfate followed by gel permeation using DEAE-Sephacel A-25, Sepharose 6B and Sephadex G-50 columns in a yield of 3 x 10 -3 %. The glycoprotein fraction showed a single band corresponding to a subunit of verectin at the same position when stained with both Coomassie brilliant blue and periodic acid-Schiff reagents on 18 % SDS-PAGE. The molecular weight (14 kDa) was confirmed by Sephadex G-50 column chromatography. The glycoprotein fraction showed a radical scavenging activity against superoxide anion generated by the xanthine-xanthine oxidase system as well as inhibition of cyclooxygenase-2 and reduction of thromboxane A 2 synthase level in vitro.

Gastroenterology and Hepatology Researchdation, and the possible putative prophylaxes to optimize cognitive decline in older adults with pre-diabetes symptom. Because of the diversities of placental extract and Aloe vera as biogenic stimulants, present review introduced the briefly selected authors' intentions in each topical subject. Specially, the influence of gut microbiota to host is astonishing and highlighting that the consequence of our behaviours affect not only the environment without, but also that within us. Based on geriatric and neurological viewpoint, present review highlights current therapeutic strategies of placenta extract and Aloe vera as the so-called "biogenic stimulants", aimed at producing a biological rejuvenator in placental extract and improving cognitive function via modifying the brain's insulin sensitivity in Aloe vera.