Radiogaschromatographie von Kohlenhydraten, Organischen Säuren und Aminosäuren aus Pflanzen

Abstract

Es wird eine Methode zur radiogaschromatographischen Bestimmung von Kohlenhydraten, organischen Säuren und Aminosäuren sowie der gleichzeitigen Ermittlung der spezifischen Aktivität von radioaktiv markierten Substanzen aus den genannten Stoffgruppen beschrieben und ihre Anwendung auf die Analyse von Pflanzenextrakten bzw. Proteinhydrolysaten dargestellt. Die Vorgabe von 0.05–0.1 mCi 14C pro g pflanzlicher Frischsubstanz genügt, um eine ausreichend hohe Markierung selbst bei Kurzzeitinkubationen (15 sec.), z.B. in der Photosyntheseforschung, zu erzielen.

On carbohydrate metabolism of cultured carrot root explants

“…Following the removal of the eluent the labelled compounds were identified by thin layer chromatography as previously described (Bender, 1984) and the 14C-activity was measured by liquid scintillation counting after elution from the thin layer chromatograms. The concentration of carbohydrates was determined by a gas chromatographic method after silylation as previously described (Pertzsch et al, 1981) (Table 2, 4, 5, 10) or by an enzymatic method following the instructions given by Boehringer (Table 6). …”

Effect of Pseudomonas fluorescens on the root exudates of two tomato mutants differently sensitive to Fe chlorosis

“…Sugar and organic acids in the fraction B were first separated with the water/chloroform mixture as described by Pertzsch et al (1981). The aqueous fraction was vacuum-dried and then dissolved in 80% (v/v) ethanol/water and passed through Analytichem Bond Elut extraction columns (ITC, Basel, Switzerland) containing strong cation (type SCX) and anion (type SAX) resins mounted in series.…”

Fixation and metabolism of carbon dioxide by photosynthetically active carrot callus cultures

“…The separated sugar, organic acid, and amino acid fractions were analyzed by means of radio gas -liquid chromatography (GLC) (Pertzsch et al 1981). Phosphate esters were separated by twodimensional thin-layer chromatography (TLC) of aliquots of the cation exchange column effluent (neutral + anionic fraction) on cellulose plates (Macherey Nagel CEL 300-10).…”

“…They show light-enhanced C 0 2 fixation (Neumann and Raafat 1973) and the carboxylating enzymes ribulosebisphosphate carboxylase (RuBPC) and phosphoenolpymvate carboxylase (PEPC) were found to be active (Kumar et al 1983). These cultures also develop the capability of growing photoautotrophically in a 5-L laboratory fermenter at ambient C 0 2 concentration, leading to substantial dry-matter production (Bender et al 1981).…”