Radioimmunoassay for somatostatin

Utsumi, M.; Makimura, Hiroyuki; Sakoda, M; Baba, Shigeaki

doi:10.1507/endocrine1927.53.3_221

Cited by 9 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pancreatic islets were isolated by a collagenase method (Taniguchi et al, 1977a) (Aguilar-Parada et al ., 1969) and somatostatin (Utsumi et al, 1977). NRS-treated groups at the 7, 35 and 70 fold dilution of the sera, respectively.…”

Section: Methodsmentioning

confidence: 94%

Role of Glucagon and Somatostatin in Insulin Release

et al. 1980

Self Cite

View full text Add to dashboard Cite

The role of endogenous glucagon and somatostatin in insulin release was studied. Islets isolated from adult rats were incubated for 60 min in 3.3, 8,3 and 16.7 mM glucose with anti-glucagon antiserum (AGA) or anti-somatostatin antiserum (ASA) which was produced in rabbits. For the control experiments the antiserum was replaced by normal rabbit serum (NRS). Insulin release from islets treated with AGA was suppressed in 3.3 and 8.3 mM glucose as compared to islets treated with NRS, while insulin release was not affected in 16.7 mM glucose. On the other hand, insulin release from islets treated with ASA was enhanced in 3.3 and 8.3 mM glucose as compared to that from islets treated with NRS, whereas in 16.7 mM glucose it was not different from the control. These observations indicate that endogenous glucagon and somatostatin play a physiological role in the regulation of insulin release, but their regulation of insulin release is deranged at non-physiologically high glucose concentrations.

show abstract

Section: Methodsmentioning

confidence: 94%

Role of Glucagon and Somatostatin in Insulin Release

et al. 1980

Self Cite

View full text Add to dashboard Cite

show abstract

“…Glucagon was determined by radioimmunoassay using glucagon antiserum (30K). Somatostatin was assayed with the method of Utsumi, Makimura, Sakoda and Baba (1977).…”

Section: Methodsmentioning

confidence: 99%

Characteristics of Release Pattern of Somatostatin and Glucagon of Pancreatic Islets Cultured in Glucose and Adenosine

Yoshioka¹,

Taniguchi²,

Tsutou³

et al. 1983

Horm Metab Res

View full text Add to dashboard Cite

The present authors reported that insulin release from cultured rat: pancreatic islets could be maintained for 27 days by raising the glucose concentration or by adding one of purine nucleosides, adeno sine, in culture medium (Tsutou 1980; Taniguchi, Tsutou and Baba 1980). However, the glucagon and somatostatin release from cul tured islets during a long period, more than 3 days, has not been reported (

show abstract

“…Synthetic cyclic somatostatin was used as a reference standard. The diluent for assay was 1 g/100ml bovine serum albumin (BSA), 0.15mol/1 NaC1, 0.002mol/1 EDTA, 0.025mol/1 benzamidine hydro-0012-186X/79/0017/0319/$01.00 [19].…”

Section: Radioimmunoassay For Somatostatinmentioning

confidence: 99%

Determination of immunoreactive somatostatin in rat plasma and responses to arginine, glucose and glucagon infusion

et al. 1979

View full text Add to dashboard Cite

A method for the determination of immunoreactive somatostatin in rat plasma is described. Blood specimens were collected into aprotinin and EDTA. Plasma was separated, immediately diluted with acidified acetone and ultrasonicated. The resultant supernatant was lyophilised. The dilution curve of the material thus extracted was parallel to that of synthetic somatostatin. The material was eluted mainly in a similar position to that of synthetic somatostatin on Sephadex G-25(0 column chromatography. The somatostatin immunoreactivity was degraded significantly from the pre-incubated value of 846 + 86 pg/ml (n = 4, mean _+ SEM) to 102 __ 16 pg/ml in the same manner as that of synthetic somatostatin when incubated with one ml of fresh rat plasma at 37 ~ for 30 min. The mean recovery in quadruplicate of immtmoreacfive somatostatin at concentrations of 100, 200 and 400 pg/ml was 83 ___ 7, 95 ___ 4 and 76 ___ 4%, respectively. Using this method, plasma immunoreactive somatostatin responses to arginine, glucose and glucagon infusion were measured in pentobarbital anaesthetized rats. The mean basal plasma immtmoreactive somatostatin concentration in the jugular vein was 35 + 3 pg/ml (n = 7), while that in the hepatic portal vein was 120 + 17 pg/ml (n = 7). Infusion of arginine, glucose and glucagon all resulted in 2-3 fold increases in portal plasma immunoreactive somatostatin concentration.

show abstract

Radioimmunoassay for somatostatin

Cited by 9 publications

References 0 publications

Role of Glucagon and Somatostatin in Insulin Release

Role of Glucagon and Somatostatin in Insulin Release

Characteristics of Release Pattern of Somatostatin and Glucagon of Pancreatic Islets Cultured in Glucose and Adenosine

Determination of immunoreactive somatostatin in rat plasma and responses to arginine, glucose and glucagon infusion

Contact Info

Product

Resources

About