Radiometric method for determination of glucose 6-phosphatase activity in liver

Meerov, G. I.; Ryzhkova, Yu.P.

doi:10.1016/0003-2697(69)90055-4

Cited by 9 publications

(5 citation statements)

References 1 publication

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“…The distribution of the small amount of endoplasmic reticulum that is present in these cells is unclear. The activity of glucose 6-phosphatase, a classical marker of this structure (Hers & de Duve, 1950;Colbeau et al, 1971), was insufficient to assay with ['4C]glucose 6-phosphate as substrate (Hubscher & West, 1965;Meerov & Ryzhkova, 1969). NADPHcytochrome c reductase had a slightly different distribution from that of the presumptive plasmamembrane markers.…”

Section: Resultsmentioning

confidence: 99%

The subcellular distribution and some properties of the cytochrome b component of the microbicidal oxidase system of human neutrophils

Segal¹,

Jones²

1979

Biochemical Journal

134

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A novel cytochrome b has recently been described in human neutrophils. The subcellular distribution of this cytochrome was investigated by analytical fractionation on continuous sucrose gradients and it was found to have a dual localization, the major component having a similar distribution to the plasma-membrane marker [3H]concanavalin A, and a denser peak located with the specific granules. The two components were separated on discontinuous gradients and studied independently.

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Section: Resultsmentioning

confidence: 99%

The subcellular distribution and some properties of the cytochrome b component of the microbicidal oxidase system of human neutrophils

Segal¹,

Jones²

1979

Biochemical Journal

134

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“…Cytochrome b was measured by reduced minus oxidized difference spectroscopy on aliquots (400 #1) of the fractions in 11.0% sucrose, and compared with that of the following components measured as follows: protein by the method of Lowry et al (20) using bovine serum albumin as standard; 5' nucleotidase by the hydrolysis of :~H-AMP (11); adenylate cyclase by the generation of cyclic AMP (cAMP) from ATP, using the method of Wisher and Evans (37), minimally modified by the addition ofguanosine triphosphate ( 10 raM) and sodium fluoride (10 mM) to the incubation medium; cAMP by a competitive binding radioassay (Cyclic-AMP Assay Kit, Amersham International, Amersham, Buckinghamshire, England); receptors for N-formyl-L-methionyl-L-leucyl-L-phenylalanine (f-Met-Leu-Phe) by a f-Met-Leu-[:JH]-Phe-binding assay (26); cytochrome c oxidase by the spectrophotometric method of Cooperstein and Lazarow (7); glucose-6-phosphatase by the production of radio-labeled phosphate from D-glucose-[~4C]-(U)-6-phosphate potassium salt (Amersham International) (13,22); lysozyme spectrophotometrically by the lysis of Micrococcus lysodeikticus (12) using egg white lysozyme (Sigma Chemical Co.) as a standard; myeloperoxidase by the peroxidation of o-dianisidine as described by Bretz and Baggiolini (5), using horse radish peroxidase (HRPO) (Type II, Sigma Chemical Co.) as a standard.…”

Section: A Natyses Conducted On Fractionated Cellsmentioning

confidence: 99%

Development of cytochrome b and an active oxidase system in association with maturation of a human promyelocytic (HL-60) cell line

Roberts¹,

Cross²,

Jones³

et al. 1982

The Journal of Cell Biology

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The human HL-60 myeloid leukaemia cell line developed, during maturational changes induced by dimethyl sulphoxide, an enhanced capacity for phorbol myristate acetatestimulated oxidative activity and acquired a cytochrome b. Titration of the absorbance at 559 nm at potentials of -190 to -370 mV indicated that this cytochrome had a very low potential, differentiating it from mitochondrial and endoplasmic reticulum cytochromes and identifying it as the cytochrome b-24s that has been recently found in other phagocytic cells. Subcellular fractionation studies of mature HL-60 cells showed that cytochrome b had a dual distribution within the cell. The lighter peak of activity was associated with the plasma membrane markers, adenylate cyclase and receptors for the N-formyl-t-methionyl-t-leucyl-t-phenylalanine (f-MetLeu-Phe) peptide. The denser components localized with the mitochondria but were distinct from mitochondrial cytochromes because whereas the activity of cytochrome c oxidase fell during HL-60 cell maturation, that of this cytochrome b was markedly increased. Concentrations of myeloperoxidase were unrelated to activity of the oxidase system and decreased as the cells matured. The increase in the concentrations of cytochrome b with cellular maturation parallelled the increase in the stimulated nonmitochondrial respiratory activity of these cells. The turnover of the hexose monophosphate shunt of immature cells was increased by the oxidising agents, methylene blue and tert-butylhydroperoxide, indicating that these immature cells have the capacity to generate the putative substrate of the oxidase system. The development of stimulated nonmitochondrial respiratory activity by maturing HL-60 cells is associated with, and is probably dependent upon, the aquisition by these cells of the cytochrome b-245 oxidase system.

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“…Glucose 6-phosphatase (EC 3.1.3.9) was assayed as described by Hubscher & West (1965) with [l4C]glucose 6-phosphate (Meerov & Ryzhkova, 1969).…”

Section: Methodsmentioning

confidence: 99%

Effect of Bile-Duct Ligation on Organelle Marker Enzymes in the Liver and Serum of Rats

Neale

Heath

1975

Clinical Science

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1. Marker enzymes for the principal subcellular organelles of rat liver were asayed in the liver of rats 1 day and 8 days after bile-duct ligation or after laparotomy as a control procedure. 2. The microsomal enzymes in liver tissue showed complex changes. Benz[alpha]pyrene hydroxylase activity, predominantly found in the smooth endoplasmic reticulum, was decreased. Glucose 6-phosphatase activity and ribonucleic acid, which are localized predominantly in the rough endoplasmic reticulum, were increased. 3. The plasma membrane enzyme, alkaline phosphatase, increased in activity after bile-duct ligation. 4. No changes in mitochondrial enzyme activities were noted after 1 day but there was a 50% reduction 8 days after ligation. Lysosomal enzyme activities did not change in the liver tissue. 5. Liver catalase and D-amino acid oxidase activities showed a slight increase at 1 day postligation but a significant fall by 8 days. 6. Lactate dehydrogenase, a cytosol enzyme, showed a decrease in activity after 1 day but an increase in tissue activities 8 days after ligation. 7. Serum activities of mitochondrial, plasma membrane, microsomal, lysosomal and cytosol marker enzymes tended to increase post-ligation, particularly at 8 days. 8. Monoamine oxidase, a predominantly mitochondrial enzyme, was greatly elevated in the serum after 1 day but had returned to normal activities by 8 days.

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Radiometric method for determination of glucose 6-phosphatase activity in liver

Cited by 9 publications

References 1 publication

The subcellular distribution and some properties of the cytochrome b component of the microbicidal oxidase system of human neutrophils

The subcellular distribution and some properties of the cytochrome b component of the microbicidal oxidase system of human neutrophils

Development of cytochrome b and an active oxidase system in association with maturation of a human promyelocytic (HL-60) cell line

Effect of Bile-Duct Ligation on Organelle Marker Enzymes in the Liver and Serum of Rats

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