Radix Tetrastigma hemsleyani flavone inhibits proliferation, migration, and invasion of human lung carcinoma A549 cells

Abstract

Radix Tetrastigma hemsleyani flavone (RTHF) is widely used as a traditional herb and has detoxification and anti-inflammatory effects. In this study, we investigated the potential effects of RTHF on the growth and metastasis of human lung adenocarcinoma A549 cells and evaluated its mechanisms. A549 cells were treated with RTHF at various concentrations for different periods. In vitro Cell Counting Kit-8 assay and colony formation methods showed that RTHF had dose- and time-dependent antiproliferation effects on A549 cells. A cell adhesion assay showed that RTHF decreased A549 cell adhesion in a dose-dependent manner. Cell invasion and migration were investigated using the Transwell assay and observed using an inverted microscope; the results showed that cell metastasis was significantly lower in the treatment group than that in the control group (P<0.01). Expression of metastasis-related matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) was detected by real-time polymerase chain reaction and Western blotting. The results showed that the expression of MMP-2, MMP-9, and TIMP-1 decreased, while that of TIMP-2 increased significantly in the RTHF group when compared with the results of the control group. These results show that RTHF exhibits antigrowth and antimetastasis activity in lung cancer A549 cells by decreasing the expression of MMP-2/-9 and TIMP-1 and increasing that of TIMP-2.

Radix <em>Tetrastigma hemsleyani</em> flavone exhibits antitumor activity in colorectal cancer via Wnt/&beta;-catenin signaling pathway

“…Furthermore, they explored and found that RTHF exhibited anti-growth and antimetastasis activity in lung cancer A549 cells by regulating the expression of MMPs and tissue inhibitors of metalloproetienases. 22 …”

The Phytochemistry, Pharmacology, and Quality Control of Tetrastigma hemsleyanum Diels & Gilg in China: A Review

“…In the in vitro experiments with different lung cancer cells (A549 cells and A431 cells), the total flavonoids of TDG can inhibit the proliferation and apoptosis of tumor cells, and its mechanism of action involves the Bcl-2 family proteins, Caspase family proteins, MAPK pathway, and the ubiquitin-proteasome pathway. Besides, the total flavonoids of TDG can markedly inhibit the metastasis and invasion of tumor cells, with migration and invasion being associated with the activation of MMPs and TIMPs proteins (Zhong et al, 2013;Zhong et al, 2016;Zhong et al, 2017;Zhong et al, 2019). The above studies indicate that the total flavonoids of TDG has a very good therapeutic effect on lung cancer at the cellular level.…”

<p>Bioinformatic analysis of miR-4792 regulates <em>Radix Tetrastigma hemsleyani</em> flavone to inhibit proliferation, invasion, and induce apoptosis of A549 cells</p>

“…Clinical studies have shown that flavonoids may have great implications in the prevention and treatment of cancer in humans 6,7. Component analyses showed that RTH is rich in flavones, and RTH flavone (RTHF) has good in vivo and in vitro effects on various cancers, including lung cancer 8,9. However, its antitumor mechanism and related regulatory pathway are still uncertain.…”