Raman and Infrared Studies of Wet-Spun Films of Na-Hyaluronate

Lee, S A; Myers, Linda; Powell, J. W.; Suleski, Thomas J.; Rupprecht, A.

doi:10.1080/07391102.1993.10508717

Cited by 9 publications

(5 citation statements)

References 20 publications

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“…In previous 13 C-NMR studies of HA in the solid state, we found evidence for significant conformational differences relative to HA in aqueous solution ,…”

mentioning

confidence: 55%

“…19 Additional evidence for a change in HA conformation upon hydration has been obtained by Brillouin and X-ray scattering, as well as Raman and infrared spectroscopy. 20,21 The conformation adopted by HA in aqueous solution is the subject of continuing study. HA is a polyanion and exhibits typical polyelectrolyte properties in response to changes in its ionic environment.…”

mentioning

confidence: 99%

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¹³C-NMR Studies of Hyaluronan: Conformational Sensitivity to Varied Environments

Cowman¹,

Hittner²,

Feder-Davis³

1996

Macromolecules

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Hyaluronan (HA) samples ranging in size from small oligosaccharides to high molecular weight polymer have been studied by 13C-NMR spectroscopy. In neutral aqueous solutions, the chemical shifts of carbons directly involved in the β-1,3 glucuronidic linkage are found to be sensitive to (1) residue linkage position in short chains, (2) oligomer degree of polymerization, (3) solvent ionic strength, and (4) monovalent vs divalent counterions. The carbons of the β-1,4-glucosaminidic linkage show less sensitivity to the above conditions. Thus conformational versatility for HA in aqueous solution is correlated with a chemical shift change primarily in carbons of the β-1,3 linkage. We have also compared the 13C spectrum of HA in neutral aqueous salt solutions to spectra observed in dimethyl sulfoxide (DMSO) solution (ordered 2- or 4-fold HA form) or the solid state (Na+ counterion, tetragonal 4-fold helical HA form). The solid state spectrum is similar to that found in DMSO but differs substantially from the aqueous solution spectrum. The differences are attributed to (1) rotation of the acetamido group, with concomitant change in hydrogen bonding and average conformation at the β-1,4 linkage, and (2) loss of hydrogen bonds in aqueous solution and consequent change in average conformation at the β-1,3 linkage.

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“…In previous 13 C-NMR studies of HA in the solid state, we found evidence for significant conformational differences relative to HA in aqueous solution ,…”

mentioning

confidence: 55%

mentioning

confidence: 99%

¹³C-NMR Studies of Hyaluronan: Conformational Sensitivity to Varied Environments

Cowman¹,

Hittner²,

Feder-Davis³

1996

Macromolecules

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“…The first IR and Raman spectra of GAGs were published more than 50 and 30 years ago,7, 8 respectively. Later studies analyzed the differences of the spectra in solid‐state and in aqueous solutions,9 or with different hydration rates10–15 or from different species (CS from different sources of cartilage, such as crocodile, shark, chicken, ray) 16. Several peaks were identified by spectral comparison,17 especially those associated with sulfate vibrations8, 18–21 and different conformations of iduronate 22–25.…”

Section: Introductionmentioning

confidence: 99%

Rapid Characterization of Glycosaminoglycans Using a Combined Approach by Infrared and Raman Microspectroscopies

Mainreck

Brézillon

Sockalingum

et al. 2011

Journal of Pharmaceutical Sciences

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“…Even at fairly low concentration, it forms highly viscous aqueous solutions. The conformation of the HA chains has been studied for some time using a couple of methods including NMR, , Raman, and infrared spectroscopy, electron microscopy, , neutron and X-ray scattering, vacuum ultraviolet circular dichroism, and computer simulation …”

Section: Introductionmentioning

confidence: 99%

Concentration and Molecular Weight Dependence of the Quenching of Ru(bpy)₃²⁺ by Ferricyanide in Aqueous Solutions of Synthetic Hyaluronan

et al. 2000

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The quenching of electronically excited Ru(bpy)3 2+ (bpy = 2,2‘-bipyridine) by ferricyanide (FC) in highly viscous aqueous solutions of synthetic hyaluronan (HA) was studied. The quenching reaction was found to occur by direct electron transfer at short times and by diffusional encounter at longer times after the excitation flash. Intrinsic and diffusional quenching rate constants were found to decrease with increasing polymer concentration but not with increasing molecular weight of HA, although the effect of both parameters on macroscopic viscosity was comparable. It was also found, that the lifetime of the sensitizer increased with increasing polymer concentration, indicating retarded quenching by oxygen. Efficient diffusion of the reactants in the aqueous pseudophase occurs even in solutions with high macroscopic viscosity. We compared two kinds of HAs (biosynthetic and animal source) in the present experiments and obtained a sharp contrast in photochemical properties between them while other observable macroscopic parameters showed no difference. Biosynthetic HA is more suitable for the systematic study to elucidate the molecular weight dependence of the present photochemical properties showing microscopic aspects of the HA solution.

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Raman and Infrared Studies of Wet-Spun Films of Na-Hyaluronate

Cited by 9 publications

References 20 publications

¹³C-NMR Studies of Hyaluronan: Conformational Sensitivity to Varied Environments

¹³C-NMR Studies of Hyaluronan: Conformational Sensitivity to Varied Environments

Rapid Characterization of Glycosaminoglycans Using a Combined Approach by Infrared and Raman Microspectroscopies

Concentration and Molecular Weight Dependence of the Quenching of Ru(bpy)₃²⁺ by Ferricyanide in Aqueous Solutions of Synthetic Hyaluronan

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Raman and Infrared Studies of Wet-Spun Films of Na-Hyaluronate

Cited by 9 publications

References 20 publications

13C-NMR Studies of Hyaluronan: Conformational Sensitivity to Varied Environments

13C-NMR Studies of Hyaluronan: Conformational Sensitivity to Varied Environments

Rapid Characterization of Glycosaminoglycans Using a Combined Approach by Infrared and Raman Microspectroscopies

Concentration and Molecular Weight Dependence of the Quenching of Ru(bpy)32+ by Ferricyanide in Aqueous Solutions of Synthetic Hyaluronan

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¹³C-NMR Studies of Hyaluronan: Conformational Sensitivity to Varied Environments

¹³C-NMR Studies of Hyaluronan: Conformational Sensitivity to Varied Environments

Concentration and Molecular Weight Dependence of the Quenching of Ru(bpy)₃²⁺ by Ferricyanide in Aqueous Solutions of Synthetic Hyaluronan