Raman spectroscopy can differentiate malignant tumors from normal breast tissue and detect early neoplastic changes in a mouse model

Kast, Rachel; Serhatkulu, Gulay K.; Cao, Alex; Pandya, Abhilash; Dai, Houbei; Thakur, J. S.; Naik, V. M.; Naik, R.; Klein, Michael D.; Auner, Gregory W.; Rabah, Raja

doi:10.1002/bip.20899

Cited by 100 publications

(88 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In breast cancer research, RS has been applied to distinguish between malignant, normal, and benign breast tissues with significant sensitivity and specificity. [15][16][17][18][19] Several groups reported HER2 amplification assessment using surfaceenhanced RS (SERS). 20,21 However, SERS requires involvement of nanoparticles and antibodies and as such, does not take full advantage of the intrinsic nature of RS.…”

Section: Introductionmentioning

confidence: 99%

Evaluating HER2 amplification status and acquired drug resistance in breast cancer cells using Raman spectroscopy

Rexer

Arteaga

et al. 2014

J. Biomed. Opt

View full text Add to dashboard Cite

Abstract. The overexpression of human epidermal growth factor receptor 2 (HER2) is associated with increased breast cancer recurrence and worse prognosis. Effective treatments such as trastuzumab and lapatinib for patients with HER2 overexpression target the blockade of HER2 signaling activities but are often limited by the emergence of acquired drug resistance. This study applied Raman spectroscopy to differentially identify the amplification status of HER2 in cells and to characterize the biochemical composition of lapatinib resistant and sensitive HER2+ breast cancer cells in response to the drug. Raman spectra from BT474 (HER2+ breast cancer cell), MCF-10A (HER2− control), and HER2+ MCF-10A (HER2+ control) were analyzed using lasso and elastic-net regularized generalized linear models (glmnet) for multivariate statistical analysis and were discriminated to groups of different HER2 expression status with an overall 99% sensitivity and specificity. Enhanced lipid content and decreased proteome were observed in HER2+ cells. With lapatinib treatment, lapatinib-resistant breast cancer cells demonstrated sustained lipogenesis compared with the sensitive cells. © The Authors.Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluating HER2 amplification status and acquired drug resistance in breast cancer cells using Raman spectroscopy

Rexer

Arteaga

et al. 2014

J. Biomed. Opt

View full text Add to dashboard Cite

show abstract

“…2). The SERS spectrum of the thiolated dsDNA1 monolayer on the NS surface shows several characteristic SERS bands at 1,346 and 1,480 cm −1 (43). The SERS mode at 737 cm −1 is weak due to the low number of adenine bases in the DNA sequence (44) (Fig.…”

mentioning

confidence: 98%

Near-infrared remotely triggered drug-release strategies for cancer treatment

Goodman

Neumann

Nørregaard

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Remotely controlled, localized drug delivery is highly desirable for potentially minimizing the systemic toxicity induced by the administration of typically hydrophobic chemotherapy drugs by conventional means. Nanoparticle-based drug delivery systems provide a highly promising approach for localized drug delivery, and are an emerging field of interest in cancer treatment. Here, we demonstrate near-IR light-triggered release of two drug molecules from both DNA-based and protein-based hosts that have been conjugated to near-infrared-absorbing Au nanoshells (SiO 2 core, Au shell), each forming a light-responsive drug delivery complex. We show that, depending upon the drug molecule, the type of host molecule, and the laser illumination method (continuous wave or pulsed laser), in vitro light-triggered release can be achieved with both types of nanoparticle-based complexes. Two breast cancer drugs, docetaxel and HER2-targeted lapatinib, were delivered to MDA-MB-231 and SKBR3 (overexpressing HER2) breast cancer cells and compared with release in noncancerous RAW 264.7 macrophage cells. Continuous wave laser-induced release of docetaxel from a nanoshell-based DNA host complex showed increased cell death, which also coincided with nonspecific cell death from photothermal heating. Using a femtosecond pulsed laser, lapatinib release from a nanoshell-based human serum albumin protein host complex resulted in increased cancerous cell death while noncancerous control cells were unaffected. Both methods provide spatially and temporally localized drug-release strategies that can facilitate high local concentrations of chemotherapy drugs deliverable at a specific treatment site over a specific time window, with the potential for greatly minimized side effects. chemotherapy | cancer | drug release | near-infrared | nanoparticle

show abstract

“…Relative to collecting broad spectra, limiting the spectral range increases acquisition rates, which is essential for mapping tissues over larger areas. Together, the chemical information in the fingerprint (500-1600 cm −1 ) and CH-stretching (2840-3000 cm −1 ) regions of Raman spectra have been used to characterize and stratify cancerous tissues with high sensitivity and selectivity [11,12].…”

Section: Introductionmentioning

confidence: 99%

Spontaneous and coherent anti-Stokes Raman spectroscopy of human gastrocnemius muscle biopsies in CH-stretching region for discrimination of peripheral artery disease

Huang¹,

Irmak²,

Lu³

et al. 2015

Biomed. Opt. Express

View full text Add to dashboard Cite

Peripheral Artery Disease (PAD) is a common manifestation of atherosclerosis, characterized by lower leg ischemia and myopathy in association with leg dysfunction. In the present study, Spontaneous and coherent anti-Stokes Raman scattering (CARS) spectroscopic techniques in CH-stretching spectral region were evaluated for discriminating healthy and diseased tissues of human gastrocnemius biopsies of control and PAD patients. Since Raman signatures of the tissues in the fingerprint region are highly complex and CH containing moieties are dense, CH-stretching limited spectral range was used to classify the diseased tissues. A total of 181 Raman spectra from 9 patients and 122 CARS spectra from 12 patients were acquired. Due to the high dimensionality of the data in Raman and CARS measurements, principal component analysis (PCA) was first performed to reduce the dimensionality of the data (6 and 9 principal scores for Raman and CARS, respectively) in the CH-stretching region, followed by a discriminant function analysis (DFA) to classify the samples into different categories based on disease severity. The CH 2 and CH 3 vibrational signatures were observed in the Raman and CARS spectroscopy. Raman and CARS data in conjunction with PCA-DFA analysis were capable of differentiating healthy and PAD gastrocnemius with an accuracy of 85.6% and 78.7%, respectively.

show abstract

Raman spectroscopy can differentiate malignant tumors from normal breast tissue and detect early neoplastic changes in a mouse model

Cited by 100 publications

References 39 publications

Evaluating HER2 amplification status and acquired drug resistance in breast cancer cells using Raman spectroscopy

Evaluating HER2 amplification status and acquired drug resistance in breast cancer cells using Raman spectroscopy

Near-infrared remotely triggered drug-release strategies for cancer treatment

Spontaneous and coherent anti-Stokes Raman spectroscopy of human gastrocnemius muscle biopsies in CH-stretching region for discrimination of peripheral artery disease

Contact Info

Product

Resources

About