Rapid and low-cost biosensor for the detection of Staphylococcus aureus

Suaifan, Ghadeer A. R. Y.; Alhogail, Sahar; Zourob, Mohammed

doi:10.1016/j.bios.2016.11.047

Cited by 158 publications

(83 citation statements)

References 35 publications

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“…Phenotypic methods, such as culture and staining, have long been the gold standard for bacterial identication, but a major limitation is that results can take up to 48 hours (or more) to become available, 15,16 and oen do not provide sufficient information to inform antimicrobial prescription. 14 In the time taken to identication of the causative bacterium, the patient will probably receive empirical therapy, oen with broad spectrum agents, the use of which is discouraged in antimicrobial stewardship programs (in favour of narrower spectrum, targeted agents) in order to minimize the use of less effective agents which may contribute to AMR.…”

Section: The Current Diagnostic Landscape 14mentioning

confidence: 99%

“…Drug resistant strains could be detected from sputum samples within 2.5 hours, with estimated one-off costs for the DNA extraction device and reader containing the magnet, electronic circuit and thermocycler of $300 and $4000, Fig. 8 Schematic representation of the processes involved in a magnetic nanoparticle-peptide probe for the detection of Staphylococcus aureus (adapted from Suaifan et al 16 ): 1 Black coloured MNPs incorporating a terminal carboxyl group are conjugated to the N-terminus of a peptide substrate for S. aureus proteases and 2 are then immobilized on a gold sensor platform; 3 when the biosensor is exposed to S. aureus, enzymatic cleavage of the amide bond between the MNP and peptide substrate releases the nanoparticle; 4 the nanoparticles are attracted to external magnets located at the back of the sensor platform and thus expose the gold coloured surface. The colour change from black to gold occurs in 1 minute and can be detected with the naked eye.…”

Section: Nucleic Acidsmentioning

confidence: 99%

“…8). 16 This system exploits the proteolytic activity of protease enzymes, which contribute to the virulence of S. aureus.…”

Section: Proteinsmentioning

confidence: 99%

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Novel diagnostics for point-of-care bacterial detection and identification

et al. 2019

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Section: The Current Diagnostic Landscape 14mentioning

confidence: 99%

Section: Nucleic Acidsmentioning

confidence: 99%

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Novel diagnostics for point-of-care bacterial detection and identification

et al. 2019

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“…The lowest detection limit of the developed sensor for Listeria was found to be 2.17 × 10 2 CFU/mL within 30 s. The detection limit of the sensor in the spiked milk was 11.7 × 10 2 CFU/mL and in the spiked meat was 13.8 × 10 1 CFU/g detected within 15 minutes and without pre-enrichment steps. A colorimetric biosensor was used for the determination of S. aureus by Suaifan et al [61]. Their experimental results showed detection limits as low as 7, 40, and 100 CFU/mL for S. aureus in pure broth culture, and that inoculated in food produces and environmental samples, respectively.…”

Section: Fluorescence Resonance Energy Transfer-based Biosensorsmentioning

confidence: 99%

Biosensors: A Novel Approach to Detect Food-borne Pathogens.

Sentürk¹,

Aktop²,

Şanlibaba³

et al. 2018

Appli Microbiol Open Access

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Foodborne pathogens affect human health negatively and are known to cause economic losses. Therefore, quick detection of foodborne pathogens and the implementation of measures to ensure their inactivation are of immense significance. Immunological, molecular, and cultural methods are frequently used in the detection of foodborne pathogens. High cost, prolonged analysis times, and the necessity of specialized personnel are some of the disadvantages of these methods. Biosensors are known as analytical devices. The use of biosensors is considered a new approach to quickly detect foodborne pathogens and their toxins. Biosensors, which are capable of converting biological, chemical, or biochemical signals into measurable electrical signals, are systems containing a biological detection material combined with a chemical or physical transducer. Different types of biosensor are being employed for detection of pathogenic bacteria. Biosensors are sensitive, fast, economical, reliable, and portable devices, and are used in many fields such as food safety, medicine, pharmacy, measurement of environmental pollution, and the military defense. Electrochemical and optical biosensors and piezoelectric immunosensors are among the most frequently used biosensors in the detection of foodborne pathogens. In this article, the principle components and requirements for an ideal biosensor, types, and their applications in food industry are summarized.

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“…For example, Suaifan et al . has designed an optical colorimetric biosensor for detection of Staphylococcus aureus and achieved 7 CFU/ml in pure culture and 40 CFU/ml in food culture as LOD 26 . (3) Mass based biosensor.…”

Section: Introductionmentioning

confidence: 99%

An integrated impedance biosensor platform for detection of pathogens in poultry products

Liu

Jasim

Abdullah

et al. 2018

Sci Rep

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This paper presents an impedance-based biosensor for rapid and simultaneous detection of Salmonella serotypes B, D, and E with very low concentration. The biosensor consists of a focusing region, and three detection regions. The cells focusing was achieved using a ramp down electroplated vertical electrode pair along with tilted thin film finger pairs that generate p-DEP forces to focus and concentrate the bacterial cells into the center of the microchannel, and direct them toward the detection region. The detection regions consist of three interdigitated electrode arrays (IDEA), each with 20 pairs of finger coated with a mixture of anti-Salmonella antibody and crosslinker to enhance the adhesion to IDEA. The impedance changes as the target Salmonella binds to the antibody. The biosensor has showed excellent performance as proven by the detection of a single Salmonella serotype B, and simultaneous detection of two Salmonella serotypes B and D with a limit of detection (LOD) of 8 Cells/ml in ready-to-eat turkey samples, the addition of focusing capability improved the measured signal by a factor of between 4–4.5, the total detection time of 45 minutes, selectivity of the sensor on different types of bacterial cells, and the ability to distinguish between dead and live cells.

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Rapid and low-cost biosensor for the detection of Staphylococcus aureus

Cited by 158 publications

References 35 publications

Novel diagnostics for point-of-care bacterial detection and identification

Novel diagnostics for point-of-care bacterial detection and identification

Biosensors: A Novel Approach to Detect Food-borne Pathogens.

An integrated impedance biosensor platform for detection of pathogens in poultry products

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