Rapid and sensitive visual detection of residual pesticides in food using acetylcholinesterase-based disposable membrane chips

Nagatani, Naoki; Takeuchi, Akihiro; Hossain, Mohammad Amir; Yuhi, Teruko; Endo, Tatsuro; Kerman, Kağan; Takamura, Yuzuru; Tamiya, Eiichi

doi:10.1016/j.foodcont.2006.05.011

Cited by 41 publications

(12 citation statements)

References 22 publications

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“…Within Europe and the USA, pesticides can only be used with prior authorisation [99,102]. Pesticide residues pose a health risk [103][104][105], and as such their presence in foodstuffs has to be monitored. According to a European Union report [106], more than 70,000 samples, covering approximately 200 food types, were tested across Europe in 2008 for pesticides and only 3.5% were above their MRL.…”

Section: Pesticidesmentioning

confidence: 99%

“…Council Regulation (EC) No 396/2005 [107] governs MRLs in Europe and the Environmental Protection Agency sets the tolerances in the USA [102]. Confirmation methods for pesticide residues are based on gas or liquid chromatography with a selective element detector [104,108,109], but these tend to be expensive, complicated to perform and time-consuming [104,108]. To reduce the number of samples requiring confirmation, screening tests can be applied whereby only samples deemed to be positive are forwarded for confirmation [110].…”

Section: Pesticidesmentioning

confidence: 99%

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Biosensors for the analysis of microbiological and chemical contaminants in food

2012

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Increases in food production and the ever-present threat of food contamination from microbiological and chemical sources have led the food industry and regulators to pursue rapid, inexpensive methods of analysis to safeguard the health and safety of the consumer. Although sophisticated techniques such as chromatography and spectrometry provide more accurate and conclusive results, screening tests allow a much higher throughput of samples at a lower cost and with less operator training, so larger numbers of samples can be analysed. Biosensors combine a biological recognition element (enzyme, antibody, receptor) with a transducer to produce a measurable signal proportional to the extent of interaction between the recognition element and the analyte. The different uses of the biosensing instrumentation available today are extremely varied, with food analysis as an emerging and growing application. The advantages offered by biosensors over other screening methods such as radioimmunoassay, enzyme-linked immunosorbent assay, fluorescence immunoassay and luminescence immunoassay, with respect to food analysis, include automation, improved reproducibility, speed of analysis and real-time analysis. This article will provide a brief footing in history before reviewing the latest developments in biosensor applications for analysis of food contaminants (January 2007 to December 2010), focusing on the detection of pathogens, toxins, pesticides and veterinary drug residues by biosensors, with emphasis on articles showing data in food matrices. The main areas of development common to these groups of contaminants include multiplexing, the ability to simultaneously analyse a sample for more than one contaminant and portability. Biosensors currently have an important role in food safety; further advances in the technology, reagents and sample handling will surely reinforce this position.

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Section: Pesticidesmentioning

confidence: 99%

Section: Pesticidesmentioning

confidence: 99%

Biosensors for the analysis of microbiological and chemical contaminants in food

2012

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“…Velasco-Garcia and Mottram (2003) presented in their review some practical applications: pH-sensitive transducers were successfully applied to the quantification of the pesticide propoxur in onion and lettuce samples, for the determination of heavy metal ions and phosphororganic pesticides in contaminated potatoes and a biosensor based on AchE immobilized onto magnetic particles in a photometric flow injection system could detect methamidophos (organophosphate insecticide) in lettuce and cabbage. More recently Nagatani et al (2007) have developed pesticide residue detection visual chips based on AchE, which could differentiate between concentrations of 0.1 and 0.2 ppm of diazinon-oxon in real samples of apple and orange juice. (2) Quality control in storage atmospheres.…”

Section: Biosensorsmentioning

confidence: 99%

Sensors for product characterization and quality of specialty crops—A review

Ruiz-Altisent

Ruiz-García

Moreda

et al. 2010

Computers and Electronics in Agriculture

192

112

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a b s t r a c tThis review covers developments in non-invasive techniques for quality analysis and inspection of specialty crops, mainly fresh fruits and vegetables, over the past decade up to the year 2010. Presented and discussed in this review are advanced sensing technologies including computer vision, spectroscopy, X-rays, magnetic resonance, mechanical contact, chemical sensing, wireless sensor networks and radiofrequency identification sensors. The current status of different sensing systems is described in the context of commercial application. The review also discusses future research needs and potentials of these sensing technologies. Emphases are placed on those technologies that have been proven effective or have shown great potential for agro-food applications. Despite significant progress in the development of non-invasive techniques for quality assessment of fruits and vegetables, the pace for adoption of these technologies by the specialty crop industry has been slow.

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“…However, poisoning still occurred from time to time presently, even the low levels of oPs within farm products also inevitably damaged the people's health due to the high toxicity, causing some chronic diseases such as breast cancer, kidney failure, eyes blindness and so on [22,23]. Even worse, the low levels of oPs was unable to be detected rapidly by microfluidic biosensor previously on site, since the trace amounts of reaction reagents required in the micro scale channel of microfluidic chip only produce weak color reaction corresponding to low absorbance as the final detection signal [24,25]. Additionally, increasing the amount of reaction reagents in the constant volume of micro scale channel was not feasible.…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Colorimetric Biosensor for Chlorpyrifos Determination based on Peroxidase-like CuFe2O4/GQDs Magnetic Nanoparticles

Mao¹,

Zuo²,

Ya³

2017

JRST

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High-precision and miniature biosensors are in urgent demand with respect to the organophosphorus pesticides used extensively in agriculture, especially for Chlorpyrifos. In this study, the novel CuFe 2 O 4 -Graphene Quantum Dots (CuFe 2 O 4 / GQDs) Magnetic Nano-Particles (MNPs) was prepared to amplify color signal as peroxidase mimetic using one-step hydrothermal method with electrostatic adsorption. Further combined with well-designed microfluidic chip containing enzyme inhibition reaction, color reaction and uV spectrophotometric detection areas, a colorimetric biosensor was developed based on CuFe 2 O 4 /GQDs for Chlorpyrifos determination. Experimental results of the biosensor displayed a linear range from 3.2 × 10 -8 M to 6 × 10 -7 M with a correlation coefficient of 0.99258 under the optimized conditions, and the Limit of Detection (LOD) of 1.07 × 10 -8 M based on S/N = 3 and the sensitivity of 855.26 (absorbance) per 1 mM. Great advantages have been exhibted in the Microfluidic colorimetric biosensor due to the small volume, and high precision for determination of low concentrations of organophosphorus pesticides, largely simplifying the tedious operation and lowing LOD, which is great benefit to the further development of low-cost, portable and high-precision biosensors.

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Rapid and sensitive visual detection of residual pesticides in food using acetylcholinesterase-based disposable membrane chips

Cited by 41 publications

References 22 publications

Biosensors for the analysis of microbiological and chemical contaminants in food

Biosensors for the analysis of microbiological and chemical contaminants in food

Sensors for product characterization and quality of specialty crops—A review

A Microfluidic Colorimetric Biosensor for Chlorpyrifos Determination based on Peroxidase-like CuFe2O4/GQDs Magnetic Nanoparticles

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