Rapid and simultaneous visual screening of SARS-CoV-2 and influenza virufses with customized isothermal amplification integrated lateral flow strip

Sun, Yong; Qin, Panzhu; He, Jun; Li, Weiwei; Shi, Yuanli; Xu, Jianguo; Wu, Qian; Chen, Qingqing; Li, Weidong; Wang, Xinxin; Liu, Guodong; Chen, Wei

doi:10.1016/j.bios.2021.113771

Cited by 28 publications

(12 citation statements)

References 42 publications

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“…In the present study, we developed the RPA-LFD and exo RPA methods for rapid detection of E. miricola . RPA-LFD and exo RPA, which have the advantages of simple operation, sensitive detection and low cost of production, were extensively used in fundamental research and industry [ 18 , 32 , 33 , 35 , 36 ]. However, the RPA method was rarely used in amphibians.…”

Section: Discussionmentioning

confidence: 99%

Rapid and sensitive detection of pathogenic Elizabethkingia miricola in black spotted frog by RPA-LFD and fluorescent probe-based RPA

Qiao

Zhang²,

Chang³

et al. 2022

Fish and Shellfish Immunology Reports

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Section: Discussionmentioning

confidence: 99%

Rapid and sensitive detection of pathogenic Elizabethkingia miricola in black spotted frog by RPA-LFD and fluorescent probe-based RPA

Qiao

Zhang²,

Chang³

et al. 2022

Fish and Shellfish Immunology Reports

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“…Such data set outcomes indicated that Ti 3 C 2 -QD-ICA had a high sensitivity and good colorimetric performance for concomitantly detecting H1N1 and SARS-CoV-2 real viruses. The method was compared with other recently reported biosensors in terms of sensitivity, reproducibility, specificity, and assay time in Table S1. ,,− Our method demonstrated a relatively superior assay performance among these assays. The Ti 3 C 2 -QD-ICA has the potential to be a valuable POCT instrument focusing upon accurate and rapid clinical determination of respiratory viruses because of its good stability, multiplex detection capability, easy operation, cheap cost, and short detection time.…”

Section: Resultsmentioning

confidence: 99%

Development of a Fluorescent Immunochromatographic Assay Based on Quantum Dot-Functionalized Two-Dimensional Monolayer Ti₃C₂ MXene Nanoprobes for the Simultaneous Detection of Influenza A Virus and SARS-CoV-2

Liu¹,

Chen³

et al. 2023

ACS Appl. Mater. Interfaces

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Accurate and rapid detection of the influenza A virus (FluA) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can effectively control their spread. We developed a colorimetric and fluorescent dual-functional two-channel immunochromatographic assay (ICA) biosensor to simultaneously detect the above-mentioned viruses. A unique two-dimensional Ti 3 C 2 -QD immunoprobe was established by adsorbing dense quantum dots (QDs) onto the light green monostromatic Ti 3 C 2 MXene surface, resulting in light green colorimetric and superior fluorescence signals and guaranteeing high sensitivity, stability, and excellent liquidity for ICA detection. Rapid visual screening for FluA and SARS-CoV-2 infections was applicable via a green colorimetric signal. Sensitive and quantitative detection of viruses in their early stages of infection was performed by using the fluorescence signal. Our proposed Ti 3 C 2 -QD-ICA biosensor can simultaneously detect 1 ng/mL or 2.4 pg/ mL FluA and 1 ng/mL or 6.2 pg/mL SARS-CoV-2 via its colorimetric or fluorescence signals, respectively, with a short testing time (20 min), good reproducibility, specificity, and accuracy. In addition, this method demonstrated sensitivity higher than that of the conventional AuNP-based ICA method in throat swab samples. Hence, our proposed Ti 3 C 2 -QD-ICA method can be potentially applied for the rapid, ultrasensitive, and multiplex detection of respiratory viruses.

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“…This is true of isothermal techniques such as the RT-LAMP, which require no thermo-cycler. Thus, RT-LAMP can be combined with an LFA [ 119 ]. In the last few years, LFAs have been adopted for the detection of genetic markers in solution, and advanced into systems that can detect circulating molecules in biological fluids without the need for an instrument.…”

Section: Discussionmentioning

confidence: 99%

A Review of the Nucleic Acid-Based Lateral Flow Assay for Detection of Breast Cancer from Circulating Biomarkers at a Point-of-Care in Low Income Countries

et al. 2022

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The current levels of breast cancer in African women have contributed to the high mortality rates among them. In South Africa, the incidence of breast cancer is also on the rise due to changes in behavioural and biological risk factors. Such low survival rates can be attributed to the late diagnosis of the disease due to a lack of access and the high costs of the current diagnostic tools. Breast cancer is asymptomatic at early stages, which is the best time to detect it and intervene to prevent high mortality rates. Proper risk assessment, campaigns, and access to adequate healthcare need to be prioritised among patients at an early stage. Early detection of breast cancer can significantly improve the survival rate of breast cancer patients, since therapeutic strategies are more effective at this stage. Early detection of breast cancer can be achieved by developing devices that are simple, sensitive, low-cost, and employed at point-of-care (POC), especially in low-income countries (LICs). Nucleic-acid-based lateral flow assays (NABLFAs) that combine molecular detection with the immunochemical visualisation principles, have recently emerged as tools for disease diagnosis, even for low biomarker concentrations. Detection of circulating genetic biomarkers in non-invasively collected biological fluids with NABLFAs presents an appealing and suitable method for POC testing in resource-limited regions and/or LICs. Diagnosis of breast cancer at an early stage will improve the survival rates of the patients. This review covers the analysis of the current state of NABLFA technologies used in developing countries to reduce the scourge of breast cancer.

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Rapid and simultaneous visual screening of SARS-CoV-2 and influenza virufses with customized isothermal amplification integrated lateral flow strip

Cited by 28 publications

References 42 publications

Rapid and sensitive detection of pathogenic Elizabethkingia miricola in black spotted frog by RPA-LFD and fluorescent probe-based RPA

Rapid and sensitive detection of pathogenic Elizabethkingia miricola in black spotted frog by RPA-LFD and fluorescent probe-based RPA

Development of a Fluorescent Immunochromatographic Assay Based on Quantum Dot-Functionalized Two-Dimensional Monolayer Ti₃C₂ MXene Nanoprobes for the Simultaneous Detection of Influenza A Virus and SARS-CoV-2

A Review of the Nucleic Acid-Based Lateral Flow Assay for Detection of Breast Cancer from Circulating Biomarkers at a Point-of-Care in Low Income Countries

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Rapid and simultaneous visual screening of SARS-CoV-2 and influenza virufses with customized isothermal amplification integrated lateral flow strip

Cited by 28 publications

References 42 publications

Rapid and sensitive detection of pathogenic Elizabethkingia miricola in black spotted frog by RPA-LFD and fluorescent probe-based RPA

Rapid and sensitive detection of pathogenic Elizabethkingia miricola in black spotted frog by RPA-LFD and fluorescent probe-based RPA

Development of a Fluorescent Immunochromatographic Assay Based on Quantum Dot-Functionalized Two-Dimensional Monolayer Ti3C2 MXene Nanoprobes for the Simultaneous Detection of Influenza A Virus and SARS-CoV-2

A Review of the Nucleic Acid-Based Lateral Flow Assay for Detection of Breast Cancer from Circulating Biomarkers at a Point-of-Care in Low Income Countries

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Development of a Fluorescent Immunochromatographic Assay Based on Quantum Dot-Functionalized Two-Dimensional Monolayer Ti₃C₂ MXene Nanoprobes for the Simultaneous Detection of Influenza A Virus and SARS-CoV-2