Rapid Detection and Identification of Antimicrobial Peptide Fingerprints of Nasal Fluid by Mesoporous Silica Particles and MALDI-TOF/TOF Mass Spectrometry: From the Analytical Approach to the Diagnostic Applicability in Precision Medicine

Preianò, Mariaimmacolata; Maggisano, Giuseppina; Murfuni, Maria Stella; Villella, Chiara; Colica, Carmela; Fregola, Annalisa; Pelaia, Corrado; Lombardo, Nicola; Pelaia, Girolamo; Savino, Rocco; Terracciano, Rosa

doi:10.3390/ijms19124005

Cited by 12 publications

(10 citation statements)

References 36 publications

(46 reference statements)

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“…Apart from NLF nasal secretions can be obtained for mass spectrometric analysis by nasal cotton swabs or filter papers. Preiano et al [84] and Lombardo et al [85] used cotton swabs and a fast procedure based on mesoporous silica particles (MPS) to enrich nasal fluids in its antimicrobial protein component in combination with MALDI-TOF/TOF MS to differentiate between healthy probands and patients with upper respiratory diseases like allergic rhinitis by proposing 'rhinomic fingerprints. ' Kim et al [86] applied filter paper in patients with CRS to collect nasal secretions for nanoLC-MS/MS on a quadrupole orbitrap mass spectrometer (Q-Exactive plus; Thermo Fisher Scientific).…”

Section: Proteomics Of the Nasal Mucusmentioning

confidence: 99%

Nasal mucus proteome and its involvement in allergic rhinitis

Tomazic

Darnhofer

Birner‐Gruenberger

2020

Expert Review of Proteomics

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Introduction: Nasal mucus is the first line defense barrier against various pathogens including allergens. Proteins in nasal mucus maybe used as biomarkers for diagnosis or future therapeutic strategies. Proteomics opens the possibility to investigate whole human proteomes. Areas Covered: We aimed to analyze the existing literature on nasal mucus and nasal secretions proteomic approaches especially in allergic rhinitis. A PubMed/Medline search was conducted entering the following keywords and combinations: "nasal mucus", "nasal lavage fluid," nasal secretions," "nasal swabs," "allergic rhinitis," "proteins," and "proteomics." Expert opinion: The majority of studies focus on single proteins or protein groups mainly using ELISA techniques. Four studies met the criteria using mass spectrometry in the analysis of nasal mucus proteomes in rhinologic diseases. In these studies, 7, 35, 267, and 430 proteins were identified, respectively. These four studies are discussed in this review and put in relation to seven other proteomic studies that focus on nasal lavage fluid and nasal secretions obtained by swabs or filter paper. To put it in a nutshell, proteomics facilitates the investigation of the nasal secretome and its role in healthy and diseased state and as potential biomarkers for new diagnostic or therapeutic approaches. ARTICLE HISTORY

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Section: Proteomics Of the Nasal Mucusmentioning

confidence: 99%

Nasal mucus proteome and its involvement in allergic rhinitis

Tomazic

Darnhofer

Birner‐Gruenberger

2020

Expert Review of Proteomics

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“…Drug loading was confirmed by matrix‐assisted laser desorption ionization mass spectrometer (MALDI‐TOF/TOF 5800 system, AB SCIEX, Framingham, MA, USA) analysis of CaNV+MB‐Drug. [ 33 ] Briefly, each sample (0.5 µL) was spotted on Opti‐TOF 384‐Well Insert (AB SCIEX, Framingham, MA, USA) with 50% acetonitrile (0.5 µL) in 0.1% trifluoroacetic acid (Merck, Darmstadt, Germany), followed by drying at room temperature. Data acquisition was conducted by processing MALDI‐TOF spectra by using Data Explorer software (version 4.11, AB SCIEX, Framingham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Cancer Patient Tissueoid with Self‐Homing Nano‐Targeting of Metabolic Inhibitor

et al. 2021

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The current paradigm of cancer medicine focuses on patient-and/or cancer-specific treatments, which has led to continuous progress in the development of patient representatives (e.g., organoids) and cancer-targeting carriers for drug screening. As breakthrough concepts, i) living cancer tissues convey intact profiles of patient-specific microenvironmental signatures. ii) The growth mechanisms of cancer mass with intense cell-cell interactions can be harnessed to develop self-homing nano-targeting by using cancer cell-derived nanovesicles (CaNVs). Hence, a tissueoid model of ovarian cancer (OC) is developed by culturing OC patient tissues in a 3D gel chip, whose microchannel networks enable perfusion to maintain tissue viability. A novel model of systemic cancer responses is approached by xenografting OC tissueoids into ischaemic hindlimbs in nude mice. CaNVs are produced to carry general chemotherapeutics or new drugs under pre/clinical studies that target the BRCA mutation or energy metabolism, thereby increasing the test scope. This pioneer study cross-validates drug responses from the OC clinic, tissueoid, and animal model by demonstrating the alignment of results in drug type-specific efficiency, BRCA mutation-dependent drug efficiency, and metabolism inhibition-based anti-cancer effects. Hence, this study provides a directional foundation to accelerate the discovery of patient-specific drugs with CaNV application towards future precision medicine.

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“…In line with our previous experiments aimed at obtaining a smart and easy protocol for fast and convenient MALDI-TOF profiling procedure [12,13,16,17,24,26,27,28], we performed a dispersive SPE.…”

Section: Resultsmentioning

confidence: 99%

Hexagonal Mesoporous Silica as a Rapid, Efficient and Versatile Tool for MALDI-TOF MS Sample Preparation in Clinical Peptidomics Analysis: A Pilot Study

et al. 2019

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Improvement in high-throughput MALDI-TOF MS analysis requires practical and efficient sample preparation protocols for high acquisition rates. The use of hexagonal mesoporous silica (HMS) sorbents in combination with MALDI-TOF MS was explored as a versatile tool for peptidomic profiling of clinical specimens difficult to process, but considered important sources of disease biomarkers: synovial fluid and sputum. A rapid and efficient procedure, based on dispersive solid-phase extraction of peptides using commercially available wormhole mesostructured HMS, was tested for: a) pre-concentration of standard peptides in serially diluted solution up to the sub-nanomolar range; b) peptidome profiling of sputum and synovial fluid. The use of HMS, as dispersed sponges, significantly amplified the peptidic repertoire of sputum and synovial fluid by excluding from the adsorptive process large size proteins, which mask and/or suppress peptidome signals. The protocol proposed, as dispersive solid phase extraction, ensures good analytical performances. Moreover, it is economical and rapid, as it avoids the use of less reproducible and prolonged sample preparation procedures, such as the use of ultrafiltration filter devices. These findings may contribute to defining a high-throughput screening MS-based platform for monitoring key peptidic features of difficult to analyse bodily fluids in a clinical setting.

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Rapid Detection and Identification of Antimicrobial Peptide Fingerprints of Nasal Fluid by Mesoporous Silica Particles and MALDI-TOF/TOF Mass Spectrometry: From the Analytical Approach to the Diagnostic Applicability in Precision Medicine

Cited by 12 publications

References 36 publications

Nasal mucus proteome and its involvement in allergic rhinitis

Nasal mucus proteome and its involvement in allergic rhinitis

Cancer Patient Tissueoid with Self‐Homing Nano‐Targeting of Metabolic Inhibitor

Hexagonal Mesoporous Silica as a Rapid, Efficient and Versatile Tool for MALDI-TOF MS Sample Preparation in Clinical Peptidomics Analysis: A Pilot Study

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