Rapid detection of chiral drug ephedrine using erythrosin B for the resonance Rayleigh scattering probe

Abstract

Ephedrine (EH) and pseudoephedrine (PEH), which are chiral enantiomers commonly used clinically, have different pharmacological actions and treatment effects due to their chiral nature. In the presence of Pd2+, the reaction system of Ery B (erythrosin B)‐Pd2+ has a strong resonance Rayleigh scattering (RRS) intensity. Adding EH into this system reduced the RRS intensity, but PEH could not produce this phenomenon. The chiral recognition of these enantiomers could be achieved according to this spectral difference. At the same time, reduction in RRS strength of the reaction system is proportional to the concentration of EH. Under optimized conditions, the linear range is 40–960 ng/ml, and the detection limit is 3.9 ng/ml. A new method for the rapid detection of EH enantiomers can be established. Based on this assay, a new method for the determination of the chiral enantiomers of EH and PEH can be developed.

Applying resonance Rayleigh scattering and spectrofluorimetric techniques for the selective determination of neomycin sulfate using Congo red as a probe: Applications for wastewater analysis

“…The detection of substances by spectrophotometry depends on the formation of coloured complexes, [50] spectrofluorimetric analysis could rely on the fluorescence of the substance itself and be detected by the substance due to a fluorescence quenching effect, [51,52] whereas RRS depends on augmentation of the RRS spectra by the complex formed. [53,54] Although these techniques have different response ranges and strengths when measuring substances, they have the same chemical reaction, that is, the ionic association reaction. In this study, the dependent complex is a combination of negatively charged dyes and protonated positively charged antibiotics.…”

Interpol Review of Drug Analysis 2019-2022

“…2019 Enantiomeric resolution of ephedrine racemic mixture using molecularly imprinted carboxylic acid functionalized resin [ 577 ]; multiphase extraction method for separation of ephedrine from pinellia ternate [ 578 ]; LCMS method for the enantiomeric separation of typical illicit drugs such as ephedrines (ie, 1S,2R(+)-ephedrine and 1R,2S(−)-ephedrine) and pseudoephedrine (ie, R,R(−)-pseudoephedrine and S,S(+)-pseudoephedrine) [ 579 ]; three compounds obtained from ephedrine (Ephedrone (methcathinone) hydrochloride and its fundamental derivatives N-acetylephedrine and N-acetylephedrone) were identified and characterized by GC-MS, NMRS, IR, Raman spectroscopy, and X-ray crystallography [ 580 ]; study of Raman spectroscopic differences between Ephedrine and pseudoephedrine using micro-Raman spectroscopy and UV resonance Raman spectroscopy [ 581 ]; 2021 electrochemical sensor for voltammetric (CV, DVP and square wave voltammetry) analysis of ephedrine in pharmaceutical dosage [ 582 ]; an ephedrine sensing method using an electrified liquid-liquid interface supported with an array of apertures micro-punched in the self-adhesive polyimide tape [ 583 ]; Birch reaction method was employed to synthesize amphetamine from ephedrine and detect the most known TLC byproduct of clandestine manufacture of amphetamines [ 584 ]; H-1 NMR method for quantification of ephedrine alkaloids (methylephedrine, ephedrine, norephedrine, norpseudoephedrine, pseudoephedrine, and methylpseudoephedrine) and ephedra herbal preparations [ 585 ]; electrochemical sensor for determination of ephedrine hydrochloride [ 586 ]; HPLC-IT/TOF-MS method for identification of impurities in chloroephedrine samples and preparation of a chloroephedrine standard [ 587 ]; method for chiral separation of ephedrine and its stereoisomers by supercritical fluid chromatography tandem mass spectrometry (SFC-MS/MS) [ 588 ]; isotope profiling of delta N-15, delta C-13, and delta H-2 isotope clusters of ephedrine/pseudoephedrine to characterize the origin of the precursor in seized methamphetamine samples [ 589 ]; calixarene based portable sensor for the direct assay of ephedrine in non-prescribed herbal supplements used as adjunctive therapy for weight loss [ 590 ]; analysis of the ephedrine in Pinellia tuber marketed products by LC-TOF/MS [ 591 ]; novel stationary phase coatings by zeolite SiO2NPs coupled with beta-cyclodextrin (beta-CD) or beta-CD/ l -phenylalanine were developed for chiral open-tubular capillary electrochromatography and applied to the chiral separation of ephedrine and pseudoephedrine [ 592 ]; method for the rapid detection of ephedrine and pseudoephedrine chiral enantiomers using erythrosin B for the resonance Rayleigh scattering probe [ 593 , 594 ]; 2022 UV–Vis spectrophotometric method to estimate ephedrine hydrochloride in pharmaceutical drugs [ 595 ]; synthesis of eight new organotin derivatives containing ephedrine-substituted dithiocarbamate ligands [ <...…”

Detection of Organophosphorus Pesticides Using Silver-Coated Gold Nanoparticles

“…Absorption and FL analyses have been around for a long time. In very recent decades, resonance Rayleigh scattering, as a sensitive and convenient analysis technique, has attracted great attention in analytical science. − Resonance Rayleigh scattering spectra can be recorded by a common fluorophotometer with a synchronous scanning mode, and resonance Rayleigh scattering has been extensively applied for the detection of different analytes. Noble-metal nanostructures have strong LSPR because of the collective oscillations of free electrons on the metal surface under incident-light radiation. , LSPR can yield several plasmon-based optical phenomena like SERS, resonance absorption, and fluorescence resonance energy transfer (FRET) .…”