Rapid electrochemical detection of coronavirus SARS-CoV-2

Chaibun, Thanyarat; Puenpa, Jiratchaya; Ngamdee, Tatchanun; Boonapatcharoen, Nimaradee; Athamanolap, Pornpat; O’Mullane, Anthony P.; Vongpunsawad, Sompong; Poovorawan, Yong; Lee, Su Yin; Lertanantawong, Benchaporn

doi:10.1038/s41467-021-21121-7

Cited by 327 publications

(303 citation statements)

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“…They can be used for gene detection of (1) gene such as nucleocapsid phosphoprotein (N-gene) [226], RdRp gene [267]; (2) antigen [234]; and (3) antibodies. The method can be easily implemented into portable devices offering simple operation and on-site detection [227]. Nanotechnology may help to improve diagnosis [268,269] using techniques such as advanced POCT (point of care tests) approaches [139].…”

Section: Discussionmentioning

confidence: 99%

“…An electrochemical biosensor based on RNA amplification using isothermal RCA and differential pulse voltammetry (DPV) was reported ( Fig. 7) [227]. It involves 4 simple steps: (1) sample collection, (2) extraction of RNA/DNA, (3) hybridization using RCA reaction, and (4) detection (Fig.…”

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 99%

“…It involves 4 simple steps: (1) sample collection, (2) extraction of RNA/DNA, (3) hybridization using RCA reaction, and (4) detection (Fig. 7) [227]. The extracted RNA/DNA was purified and separated using magnetic beads coated with streptavidin.…”

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 99%

“…This assay offers a LOD of 1 copy/μL of N and S genes (Fig. 7) [227]. This method is a one-step sandwich hybridization assay, rapid (2 h), sensitive, and accurate (100% concordance result with qRT-PCR).…”

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 99%

“…This method is a one-step sandwich hybridization assay, rapid (2 h), sensitive, and accurate (100% concordance result with qRT-PCR). Furthermore, the potentiostat (PalmSen4) is portable, offering on-site, real-time diagnostic tests for COVID-19 [227]. Nucleic acid analysis using the colorimetric method was also reported.…”

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 99%

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Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Abdelhamid

Badr

2021

Nanotechnol. Environ. Eng.

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A sensitive method for diagnosing coronavirus disease 2019 (COVID-19) is highly required to fight the current and future global health threats due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV 2). However, most of the current methods exhibited high false-negative rates, resulting in patient misdiagnosis and impeding early treatment. Nanoparticles show promising performance and great potential to serve as a platform for diagnosing viral infection in a short time and with high sensitivity. This review highlighted the potential of nanoparticles as platforms for the diagnosis of COVID-19. Nanoparticles such as gold nanoparticles, magnetic nanoparticles, and graphene (G) were applied to detect SARS-CoV 2. They have been used for molecular-based diagnosis methods and serological methods. Nanoparticles improved specificity and shorten the time required for the diagnosis. They may be implemented into small devices that facilitate the self-diagnosis at home or in places such as airports and shops. Nanoparticles-based methods can be used for the analysis of virus-contaminated samples from a patient, surface, and air. The advantages and challenges were discussed to introduce useful information for designing a sensitive, fast, and low-cost diagnostic method. This review aims to present a helpful survey for the lesson learned from handling this outbreak to prepare ourself for future pandemic.

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Section: Discussionmentioning

confidence: 99%

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 99%

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 99%

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 99%

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 99%

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Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Abdelhamid

Badr

2021

Nanotechnol. Environ. Eng.

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Advanced Point‐of‐Care Testing Technologies for Human Acute Respiratory Virus Detection

et al. 2021

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system leading to respiratory tract infections (respiratory viruses, RVs). These diseases have the highest morbidity and fatality rates among acute infectious diseases and have caused a series of pandemics throughout human history. The first recorded RV occurred in 1200 BC, and subsequent major RVs have included the 1918 influenza virus, [1] severe acute respiratory syndrome coronavirus (SARS-CoV-1), [2][3][4][5] 2009 swine flu (H1N1), [6] Middle East respiratory syndrome (MERS) coronavirus, [7,8] and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), [9,10] which together have cost billions of lives (Figure 1). [11,12] The epidemics mentioned above impose a significant burden on healthcare systems and the global economy. [13,14] The recurrence of RV epidemics could result from their ubiquitous transmission routes, including direct transmission by sneezing, airborne/aerosol transmission by droplets from infected persons, and transmission by hand-to-eye/nose/mouth contact spreading RVs potentially from contaminated surfaces (handles, elevators, commonly used shared areas, and shopping bags) to an uninfected person. [15][16][17] The major RV types are influenza virus, coronavirus (CoV), adenovirus, respiratory syncytial virus (RSV), rhinovirus,The ever-growing global threats to human life caused by the human acute respiratory virus (RV) infections have cost billions of lives, created a significant economic burden, and shaped society for centuries. The timely response to emerging RVs could save human lives and reduce the medical care burden. The development of RV detection technologies is essential for potentially preventing RV pandemic and epidemics. However, commonly used detection technologies lack sensitivity, specificity, and speed, thus often failing to provide the rapid turnaround times. To address this problem, new technologies are devised to address the performance inadequacies of the traditional methods. These emerging technologies offer improvements in convenience, speed, flexibility, and portability of point-of-care test (POCT). Herein, recent developments in POCT are comprehensively reviewed for eight typical acute respiratory viruses. This review discusses the challenges and opportunities of various recognition and detection strategies and discusses these according to their detection principles, including nucleic acid amplification, optical POCT, electrochemistry, lateral flow assays, microfluidics, enzyme-linked immunosorbent assays, and microarrays. The importance of limits of detection, throughput, portability, and specificity when testing clinical samples in resource-limited settings is emphasized. Finally, the evaluation of commercial POCT kits for both essential RV diagnosis and clinical-oriented practices is included.

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Emerging Biosensing Technologies for the Diagnostics of Viral Infectious Diseases

et al. 2022

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Despite these viruses emerging in different geographic areas, they have become an undeniable threat to worldwide public health and economic burden. The coronaviruses (CoVs) include severe acute respiratory syndrome (SARS-CoV), Middle East respiratory syndrome (MERS-CoV), and especially the most recent SARS-CoV variant, SARS-CoV-2, which has caused the virulent, airborne, and infectious coronavirus disease 2019 (COVID-19) that continues to affect millions of people, resulting in the ongoing pandemic outbreak. [3,[6][7][8][9][10] Considering that most of the described viral-based community spread infections occur through aerosols and fomites [11] transmitted by presymptomatic and asymptomatic people, [12] diagnostic methods for the early detection with high accuracy and on-site capability are crucial and would further aid the current needs of the epidemic outbreak. [13][14][15] In clinical methodology, the emerging mutations and the evolution of SARS-CoV-2 isolates are determined by DNA sequencing of bronchoalveolar lavage fluid (BALF) samples. [6,16] These BALF samples come in the form of nasopharyngeal swabs or sputum samples, and the viral nucleic acid detection is performed by reverse transcription-polymerase chain Several viral infectious diseases appear limitless since the beginning of the 21 st century, expanding into pandemic lengths. Thus, there are extensive efforts to provide more efficient means of diagnosis, a better understanding of acquired immunity, and improved monitoring of inflammatory biomarkers, as these are all crucial for controlling the spread of infection while aiding in vaccine development and improving patient outcomes. In this regard, various biosensors have been developed recently to streamline pathogen and immune response detection by addressing the limitations of traditional methods, including isothermal amplification-based systems and lateral flow assays. This review explores state-of-the-art biosensors for detecting viral pathogens, serological assays, and inflammatory biomarkers from the material perspective, by discussing their advantages, limitations, and further potential regarding their analytical performance, clinical utility, and point-ofcare adaptability. Additionally, next-generation biosensing technologies that offer better sensitivity and selectivity, and easy handling for end-users are highlighted. An emerging example of these next-generation biosensors are those powered by novel synthetic biology tools, such as clustered regularly interspaced short palindromic repeats (CRISPR) with CRISPR-associated proteins (Cas), in combination with integrated point-of-care devices. Lastly, the current challenges are discussed and a roadmap for furthering these advanced biosensing technologies to manage future pandemics is provided.

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Rapid electrochemical detection of coronavirus SARS-CoV-2

Cited by 327 publications

References 50 publications

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Advanced Point‐of‐Care Testing Technologies for Human Acute Respiratory Virus Detection

Emerging Biosensing Technologies for the Diagnostics of Viral Infectious Diseases

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