Rapid Evaluation of Spermidine from 12 Bean Cultivars by Direct Real-Time Mass Spectrometry Analysis

Abstract

The routine spermidine (SPD) detection method is time-consuming and laborious due to the lengthy chromatographic separation and/or tedious sample derivatization pretreatment. In this study, direct analysis in real-time ionization mode coupled with mass spectrometry (DART-MS) was developed to rapidly determine the SPD content of 12 bean cultivars. The results were compared in detail with those of the classical UHPLC-ESI-QTOF method. After conducting a series of optimizations, a simple sample extraction procedure employing 80% aqueous methanol, was followed by determination of sample extracts directly without any chromatographic separation or prior derivatization. The validated method showed excellent performance with low limits of detection (LOD of 0.025 mg·kg−1) and good recovery rates (102.79–148.44%). The investigation highlighted that the DART-MS method (~1.3 min per three samples) could be used as a high-throughput alternative to the classic UHPLC-ESI-QTOF method (~15 min per three samples).

Plasma‐based ambient mass spectrometry: Recent progress and applications

“…Applications of plasma‐based AMS for the direct analysis of plants, animals, and related substances have been exclusively explored in the past 4 years. For example, the quantitative analysis of spermidine (SPD) in beans was realized by DART‐MS without the tedious derivatization steps (Wu et al, 2018). The low LOD (0.025 mg kg −2 ) and low matrix effect demonstrated that this method can be a better alternative to the conventional UHPLC‐ESI‐QTOF method.…”

Wheat germ, a byproduct of the wheat milling industry, as a beneficial source of anti‐aging polyamines: A quantitative comparison of various forms

“…Table 4 was used to categorize the polyamine extraction techniquesinto four groups: EM-PA, EM-CA, EM-TCA, and EM-CAM. The use of an acidic solution, such as perchloric acid, tricholoroacetic acid, or citric acid, forms the basis of all polyamine extraction techniques.The measurement of polyamines in plant tissues(Sánchez-López et al, 2009), foods(Wu et al, 2018), human blood, and urine(Häkkinen et al, 2013;Lee et al, 2021;Magnes et al, 2014) has been the subject of numerous papers. Perchloric acid was recommended in these papers as the ideal solvent for polyamine extraction in its measuring techniques.…”