Rapid identification of fatty acid methyl esters using a multidimensional gas chromatography–mass spectrometry database

Population dependent effects of photoperiod on diapause related physiological traits in an invasive beetle (Leptinotarsa decemlineata)

“…Fatty acid methyl esters (FAME) were analyzed by GC-MS using a HP 6890 GC equipped with Agilent DB-23 capillary column (30 m, 0.25 mm ID, 0.25 Ǎm phase thickness) and a HP 5973 Mass Selective Detector, Global Medical Instrumentation Inc. Ramsey Minnesota). The modified timer program of Härtig et al (2008) was used to separate fatty acids from each other. The different FAs were identified by retention time and by MS spectra.…”

Evaluation of ionic liquid stationary phases for one dimensional gas chromatography–mass spectrometry and comprehensive two dimensional gas chromatographic analyses of fatty acids in marine biota

“…Since the DB-WAX column does not allow the separation of cis and trans fatty acid isomers, this column was not further evaluated. Peaks in the chromatograms were identified using the mass spectra, relative retention time information and literature data [39,40].…”

“…Different compound classes can be easily identified using the base peaks at m/z 74, 55, 67 and 79, characteristic for saturated fatty acids (SFA), mono-unsaturated fatty acids (MUFA), di-unsaturated fatty acids (DUFA) and polyunsaturated fatty acids (PUFA), respectively [39,40]. The characteristic ion ratios "m/z 150 > m/z 149 and "m/z 80 > m/z 93 were used to confirm C18:3n6 (n6 terminal moiety) [40].…”

“…The characteristic ion ratios "m/z 150 > m/z 149 and "m/z 80 > m/z 93 were used to confirm C18:3n6 (n6 terminal moiety) [40].…”

Establishment of reliable mass spectra and retention indices library: Identification of fatty acids in human plasma without authentic standards

“…The widely available identification method is mass spectral and retention indices library searching [2] or comparison of mass spectra and retention indices with the results of authentic standards. In practice, many mass spectra and retention indices libraries are available to the analysts [3][4][5]. Among them, the library established by National Institute of Standards and Technology (NIST) [5] is the commonly used one.…”