Rapid isolation method of animal mitochondrial DNA by the alkaline lysis procedure

Kost, A. A.; Ivanov, Mikhail

doi:10.1007/bf00553878

Cited by 44 publications

(50 citation statements)

References 53 publications

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“…Somatic tissues from a single female S. constricta were collected from the Zhejiang province and were excised and stored at -70 • C. Mitochondrial DNA was isolated and purified (as described and referenced in Tamura and Aptsuka, 1988) from the somatic tissues. PCR amplification of a portion of cox1 was performed Oceanol.…”

Section: Methodsmentioning

confidence: 99%

The complete DNA sequence of the mitochondrial genome of Sinonovacula constricta (Bivalvia: Solecurtidae)

Zheng

Niu

2010

Acta Oceanol. Sin.

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The nucleotide sequence of the mitochondrial genome of the solecurtidae Bivalvia mollusca Sinonovacula constricta (GenBank accession number EU880278) has been determined and is reported here. We determined the complete mitochondrial genome sequence using long-PCR and Shot Gun Sequencing. Contained within the 17 225 base pairs (bp) are the two ribosomal RNA genes and 12 protein coding genes typical of metazoan mitochondrial genomes. The S. constricta mitochondrial DNA (mtDNA) did not contain a gene for atp8, similar to the mtDNA of Crassostrea virginica, Crassostrea giga and Mytilus edulis. The S. constricta mtDNA is 67.0% A+T (A 25.9%, C 10.5%, G 22.5%, and T 41.1%). This value is higher than that for many invertebrate mitochondrial genomes. Only 19 putative tRNA genes are present in S. constricta and 27 noncoding regions, of which two are large in size. The trnE and trnW genes as well as a second trnS were absent in S. constricta. The gene arrangement of S. constricta is different from the other Bivalvia genomes.

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Section: Methodsmentioning

confidence: 99%

The complete DNA sequence of the mitochondrial genome of Sinonovacula constricta (Bivalvia: Solecurtidae)

Zheng

Niu

2010

Acta Oceanol. Sin.

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“…Sample, DNA extraction, and PCR Specimens of C. destructor were collected from Dwyers Creek in the Grampian Ranges, located in southwestern Victoria (37jS, 142jE). Mitochondrion-enriched DNA extracts were obtained from frozen specimens following Tamura and Aotsuka (1988). Using species-specific primers designed from partial lrRNA and CO1 sequence data (GenBank Accession numbers AY191769 and AY153891) the entire mitochondrial genome for C. destructor was amplified by long-PCR in two overlapping fragments.…”

Section: Methodsmentioning

confidence: 99%

Complete mitochondrial DNA sequence of the Australian freshwater crayfish, Cherax destructor (Crustacea: Decapoda: Parastacidae): a novel gene order revealed

Miller

Nguyen

Burridge

et al. 2004

Gene

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“…The mtDNA was extracted from kidney and liver by the method of Tamura and Aotsuka (1988). The mtDNA of each animal was digested using 15 restriction Some mtDNA samples could not be extracted purely from tissue samples because of the bad conditions when we took samples in the field, so we could not detect the mtDNA fragments by the above methods.…”

Section: Analysis Of Mtdna Rflpsmentioning

confidence: 99%

Population Structure of Wild Musk Shrews (Suncus murinus) in Asia Based on Mitochondrial DNA Variation, with Research in Cambodia and Bhutan

Kurachi

BA-Loc²,

Dang

et al. 2007

Biochem Genet

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The musk shrew (Suncus murinus) is a small mammalian species belonging to Insectivora. It is widely distributed in Asia. To identify the genetic relationship among wild musk shrew populations and examine its migration route, we investigated the populations of Cambodia and Bhutan by using mitochondrial DNA restriction fragment length polymorphism analysis and compared them with other Asian populations previously described. Four haplotypes were detected in Cambodia and eight in Bhutan. A total of 53 haplotypes were detected in Asia and were classified largely into two groups, the Continental and Island types, based on a minimum spanning network. From the distribution of mtDNA types in wild musk shrews, three major population groups are identified in Asia: South Asia, Southeast Asia, and Malay. It is suggested that the Malay population group was a mix of South and Southeast Asian population groups and that this was a contact area of the two groups. In addition, other contact areas between the South and Southeast Asian groups exist in Myanmar, but unlike the Malay, the Myanmar area was the border of these groups.

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Rapid isolation method of animal mitochondrial DNA by the alkaline lysis procedure

Cited by 44 publications

References 53 publications

The complete DNA sequence of the mitochondrial genome of Sinonovacula constricta (Bivalvia: Solecurtidae)

The complete DNA sequence of the mitochondrial genome of Sinonovacula constricta (Bivalvia: Solecurtidae)

Complete mitochondrial DNA sequence of the Australian freshwater crayfish, Cherax destructor (Crustacea: Decapoda: Parastacidae): a novel gene order revealed

Population Structure of Wild Musk Shrews (Suncus murinus) in Asia Based on Mitochondrial DNA Variation, with Research in Cambodia and Bhutan

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