Rapid metallization of lambda DNA and DNA origami using a Pd seeding method

Geng, Yanli; Liu, Jianfei; Pound, Elisabeth; Gyawali, Shailendra; Harb, John N.; Woolley, Adam T.

doi:10.1039/c1jm11932j

Cited by 50 publications

(62 citation statements)

References 38 publications

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“…[3][4][5][6] For instance, Braun and co-workers firstly reported that Ag + and Au 3 + could be sequentially reduced on preselected sections of DNA by RecAmediated homologous recombination, which has vital implication to the improvement of conductive DNA nanowires. [7] Yet, the preparation of DNA nanowires has been limited to large DNA scaffolds (> 1 mm), and challenges still remain for direct deposition of the metal (e.g., Ag, [8][9][10] Au, [11,12] Pd, [13,14] and Cu [9,15] ) at appointed sequences of the DNA molecule. The main reason lies in that there is less understanding about the controllable synthesis of DNA-templated metal nanoparticles (NPs) by varying length, sequence, and even conformational state of DNA.…”

Section: Introductionmentioning

confidence: 99%

Sequence‐Dependent dsDNA‐Templated Formation of Fluorescent Copper Nanoparticles

Song

Shi

et al. 2014

Chemistry A European J

108

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There are only a few systematic rules about how to selectively control the formation of DNA-templated metal nanoparticles (NPs) by varying sequence combinations of double-stranded DNA (dsDNA), although many attempts have been made. Herein, we develop a facile method for sequence-dependent formation of fluorescent CuNPs by using dsDNA as templates. Compared with random sequences, AT sequences are better templates for highly fluorescent CuNPs. Other specific sequences, for example, GC sequences, do not induce the formation of CuNPs. These results shed light on directed DNA metallization in a sequence-specific manner. Significantly, both the fluorescence intensity and the fluorescence lifetime of CuNPs can be tuned by the length or the sequence of dsDNA. In order to demonstrate the promising practicality of our findings, a sensitive and label-free fluorescence nuclease assay is proposed.

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Section: Introductionmentioning

confidence: 99%

Sequence‐Dependent dsDNA‐Templated Formation of Fluorescent Copper Nanoparticles

Song

Shi

et al. 2014

Chemistry A European J

108

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“…37 With recent development of DNA nanotechnology, 38 for instance, a large number of 2D and 3D nanostructures can be obtained through Watson-Crick base pairing [39][40][41] or DNAprotein conjugation. Recently, remarkable 3-branch silver nanoobjects have been produced by using T-shaped DNA origamis as templates, 43,44 although the authors did not describe higher degrees of branching. In particular, there is no available methodology for preparing branched metal nanostructures with an easily variable and controllable degree of branching.…”

Section: Introductionmentioning

confidence: 99%

Preparation of one- to four-branch silver nanostructures of various sizes by metallization of hybrid DNA–protein assemblies

et al. 2013

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We exploit the versatility of DNA-protein assemblies to generate branched metal nanostructures, referred to as nanoshurikens, of various sizes and degrees of branching. Branched silver nanostructures are prepared by metallization of star-shaped DNA-protein templates composed of monobiotinylated DNA molecules surrounding a single streptavidin protein core. DNA-protein templates are prepared by direct assembly that results in a mixture of 1-, 2-, 3-and 4-branch structures that can be separated by gel electrophoresis. A one-pot, bulk metallization is then performed in situ by successive addition of silver nitrate and sodium borohydride. This results in branched metal nanostructures with one to four branches of a well-defined length that is about 3-fold shorter than that of the template. We show that it is possible to tune two structural parameters: (i) the degree of branching by varying the concentration of streptavidin and (ii) the branch length (from 21 AE 5 nm to 107 AE 22 nm) by using biotinylated DNA molecules of different sizes. † Electronic supplementary information (ESI) available: Sequences of B-DNA206, B-DNA541 and B-DNA978; effect of salt on the distribution of DNA nanostar templates (Fig. S1). See

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“…Later in the same year, Woolley et al published another Pd-based method for rapid DNA origami metallization [89]. In this paper, they not only reduced the process time, but also managed to increase the metallized particle density on the surface, as shown in Figure 3b.…”

Section: Chemical Metallization Of Dna Nanostructuresmentioning

confidence: 98%

“…The origami shape before metallization is presented in the inset (scale bar 200 nm) [88]. ( b ) DNA origami seeded with Pd 2+ and metallized with Au and the corresponding EDX results [89]. ( c , d ) A circuit-like DNA origami metallized with Au (c) and Cu (d) with respective EDX as the inset [90].…”

Section: Figurementioning

confidence: 99%

See 1 more Smart Citation

Metallic Nanostructures Based on DNA Nanoshapes

et al. 2016

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Metallic nanostructures have inspired extensive research over several decades, particularly within the field of nanoelectronics and increasingly in plasmonics. Due to the limitations of conventional lithography methods, the development of bottom-up fabricated metallic nanostructures has become more and more in demand. The remarkable development of DNA-based nanostructures has provided many successful methods and realizations for these needs, such as chemical DNA metallization via seeding or ionization, as well as DNA-guided lithography and casting of metallic nanoparticles by DNA molds. These methods offer high resolution, versatility and throughput and could enable the fabrication of arbitrarily-shaped structures with a 10-nm feature size, thus bringing novel applications into view. In this review, we cover the evolution of DNA-based metallic nanostructures, starting from the metallized double-stranded DNA for electronics and progress to sophisticated plasmonic structures based on DNA origami objects.

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Rapid metallization of lambda DNA and DNA origami using a Pd seeding method

Cited by 50 publications

References 38 publications

Sequence‐Dependent dsDNA‐Templated Formation of Fluorescent Copper Nanoparticles

Sequence‐Dependent dsDNA‐Templated Formation of Fluorescent Copper Nanoparticles

Preparation of one- to four-branch silver nanostructures of various sizes by metallization of hybrid DNA–protein assemblies

Metallic Nanostructures Based on DNA Nanoshapes

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