Rapid Method for Paper Chromatography of Organic Acids

Denison, Frank W.; Phares, E.F.

doi:10.1021/ac60070a035

Cited by 90 publications

(22 citation statements)

References 9 publications

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“…(Nordmann & Nordmann, 1960) and (b) diethyl etheracetic acid-water (13:3:1, by vol.) (Denison & Phares, 1952 For the separation of 2,4-dinitrophenylhydrazones of keto acids the following solvents were used: (c) methanolbutan-l-ol-25% (v/v) NH3 (25:10:2, by vol.) (Rasmussen, 1967) (Dancis, Hutzler & Mortimer, 1963).…”

Section: Methodsmentioning

confidence: 99%

Bacterial metabolism of 4-chloro-2-methylphenoxyacetate. Formation of glyoxylate by side-chain cleavage

Gaunt

1971

Biochemical Journal

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Crude extracts of Pseudomonas sp. grown on 4-chloro-2-methylphenoxyacetate as sole source of carbon were shown to oxidize 4-chloro-2-methylphenoxyacetate to 5-chloro-o-cresol and glyoxylate. A labelled 2,4-dinitrophenylhydrazone was isolated from an incubation mixture in which 4-chloro-2-methylphenoxy[carboxy-14C]acetate was used. The hydrazone was shown to behave identically on thin-layer chromatograms with the authentic 2,4-dinitrophenylhydrazone of glyoxylate. Radioactivity assay showed that 82% of the side chain of 4-chloro-2-methylphenoxyacetate was recovered as glyoxylate.

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Section: Methodsmentioning

confidence: 99%

Bacterial metabolism of 4-chloro-2-methylphenoxyacetate. Formation of glyoxylate by side-chain cleavage

Gaunt

1971

Biochemical Journal

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“…The tricarboxylic acid cycle compounds were extractec -or 48 hours with ether and chromatographed according to Denison and Pha S. 10 Conventional Warburg techniques were used for quantitative respiration studies.…”

Section: !T0che•lfcal Methodsmentioning

confidence: 99%

Biochemical and Physical Changes in Shaken Suspensions of Pasteurella Pestis

Wessman¹,

Miller²

1966

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“…Following incubation for 7 days at 2 5 O C., growth and residual silicate were removed by filtration and the culture solution was eva o-8 drops of water and ap lied to paper chromatograms, spotting 6 times. Papers were developet with ether-acetic acid-water (13 : 3 : I v/v) (Denison and Phares, 1952) by the descending method for 16 hr. After drying, the papers were sprayed with 8-hyroxyquinoline (0.5 per cent.…”

Section: Methodsmentioning

confidence: 99%

The Microbilogy of Rocks and Weathered Stones

Webley

Henderson

Taylor

1963

Journal of Soil Science

130

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A study has been made of the microbiology of rock surfaces and weathered stones. Increasing numbers of micro-organisms were found with increasing degree of colonization by lichens, for example, of rock surfaces. The highest numbers were generally found in 'raw' soil in rock crevices where the organic-matter content was high. Bacteria, actinomycetes, and fungi were also found in the interior of porous weathered stones but not in unweathered stones. A high proportion of the organisms isolated were able to render silicates soluble when tested in pure culture in the laboratory.

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Rapid Method for Paper Chromatography of Organic Acids

Cited by 90 publications

References 9 publications

Bacterial metabolism of 4-chloro-2-methylphenoxyacetate. Formation of glyoxylate by side-chain cleavage

Bacterial metabolism of 4-chloro-2-methylphenoxyacetate. Formation of glyoxylate by side-chain cleavage

Biochemical and Physical Changes in Shaken Suspensions of Pasteurella Pestis

The Microbilogy of Rocks and Weathered Stones

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