Rapid monitoring of CFP-10 during culture of Mycobacterium tuberculosis by using a magnetophoretic immunoassay

Kim, Jeonghyo; Lee, Jae‐Wook; Lee, Kang-In; Park, Tae Jung; Kim, Hwa Jung; Lee, Jaebeom

doi:10.1016/j.snb.2012.11.011

Cited by 32 publications

(20 citation statements)

References 22 publications

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“…These processes are highly sensitive, but time -consuming technique since it requires over 10 days for TB diagnosis. 32 To make it worse, these techniques are not suitable to distinguish mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM). 33 Thus, in order to overcome these disadvantages, many different TB sensing systems have been developed to show rapid detection, and high sensitivity and selectivity using direct clues, e.g., secreted antigens of TB virus such as CFP-10 biomarker.…”

Section: Resultsmentioning

confidence: 99%

Plasmon-Induced Photoluminescence Immunoassay for Tuberculosis Monitoring Using Gold-Nanoparticle-Decorated Graphene

Lee

Kim

Ahmed

et al. 2014

ACS Appl. Mater. Interfaces

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Metal-nanoparticle-functionalized graphene, in particular, graphene sheets containing Au nanoparticles (Au NPs), have generated considerable interest because of their unique optical and electrical characteristics. In this study, we successfully produced graphene sheets decorated with Au NPs (AuGrp) using phytochemicals as reducing agents. During this reaction, Au ions intercalated into the layered graphene flakes and were then reduced into NPs, exfoliating the graphene sheets. The physicochemical properties of the AuGrp nanocomposites were characterized, and the exfoliation process was investigated using a molecular dynamics simulation of Au NPs between graphene sheets. Our proposed technique is advantageous because the phytochemicals are mild reducing agents that preserve the graphene structure during exfoliation and NP decoration. The dispersity of the NPs on the graphene sheets was drastically improved due to the use of metal-ion intercalation. Moreover, the electrical conductivity was 6-30 times higher than that of bare graphene and reduced graphene oxide. Using antibody (Ab) modified AuGrp sheets and quantum dots, a plasmonic-induced photoluminescence immunoassay of tuberculosis (TB) antigen (aG) CFP-10 was demonstrated for a potential application of these materials. The enhancement of photoluminescence (PL) response was monitored depending on the various TB aG concentrations from 5.1 pg/mL to 51 μg/mL, and the detection limit for CFP-10 was 4.5 pg/mL. Furthermore, the selectivity was demonstrated with Ag85 as the other TB aG, and PL enhancement was not observed in this case. Therefore, AuGrp-based immunoassay showed the potential for biosensor application.

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Section: Resultsmentioning

confidence: 99%

Plasmon-Induced Photoluminescence Immunoassay for Tuberculosis Monitoring Using Gold-Nanoparticle-Decorated Graphene

Lee

Kim

Ahmed

et al. 2014

ACS Appl. Mater. Interfaces

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“…3 The sandwichtype of immunoassay is popular since it is based on nanomaterials substrates. [4][5][6][7] Recently, multiplex immunoassay using nanomaterials have attracted significant interest. [8][9][10][11][12] Especially, SERS-fluorescence dual mode nanoprobes were expected to improve the imaging quality and detection sensitivity of the fluorescence signal, quick indicator; meanwhile SERS could help to distinguish specific targets in multiplex interactions.…”

Section: Introductionmentioning

confidence: 99%

Dual-Mode SERS-Fluorescence Immunoassay Using Graphene Quantum Dot Labeling on One-Dimensional Aligned Magnetoplasmonic Nanoparticles

Zou

Zhou

Tran

et al. 2015

ACS Appl. Mater. Interfaces

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106

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A novel dual-mode immunoassay based on surface-enhanced Raman scattering (SERS) and fluorescence was designed using graphene quantum dot (GQD) labels to detect a tuberculosis (TB) antigen, CFP-10, via a newly developed sensing platform of linearly aligned magnetoplasmonic (MagPlas) nanoparticles (NPs). The GQDs were excellent bilabeling materials for simultaneous Raman scattering and photoluminescence (PL). The one-dimensional (1D) alignment of MagPlas NPs simplified the immunoassay process and enabled fast, enhanced signal transduction. With a sandwich-type immunoassay using dual-mode nanoprobes, both SERS signals and fluorescence images were recognized in a highly sensitive and selective manner with a detection limit of 0.0511 pg mL(-1).

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“…Their functionalization with different reactive groups, especially antibodies, makes them useful to bind and pre-concentrate a variety of targets under magnetic actuation, through immunomagnetic separation. Moreover, the pre-concentration of the target from the complex sample is easily achieved, offering an attractive technology to be implemented in magneto-actuated platforms including biosensors [10], microfluidic platforms [11], among others [12].…”

Section: Introductionmentioning

confidence: 99%

Magneto-actuated immunoassay for the detection of Mycobacterium fortuitum in hemodialysis water

Brugnera

Bundalian

Laube

et al. 2016

Talanta

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This paper addresses a sensitive method for the detection of mycobacteria in hemodialysis water samples based on a magneto-actuated immunoassay with optical readout. In this approach, micro (2.8μm) sized magnetic particles were modified with an antibody against the lipoarabinomannan (LAM) located in the mycobacterial cell wall. The system relies on the immunocapturing of the mycobacteria with the tailored antiLAM magnetic particles to pre-concentrate the bacteria from the hemodialysis samples throughout an immunological reaction. The performance of the immunomagnetic separation on the magnetic carrier was evaluated using confocal microscopy to study the binding pattern, as well as a magneto-actuated immunoassay with optical readout for the rapid detection of the bacteria in spiked hemodialysis samples. In this approach, the antiLAM polyclonal antibody was labeled with fluorescein isothiocyanate. The optical readout was achieved by the incubation with a secondary anti-fluorescein antibody labeled with peroxidase as optical reporter. The magneto-actuated immunoassay was able to detect mycobacteria contamination in hemodialysis water at a limit of detection of 13CFUmL(-1) in a total assay time of 3h without any previous culturing pre-enrichment step.

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Rapid monitoring of CFP-10 during culture of Mycobacterium tuberculosis by using a magnetophoretic immunoassay

Cited by 32 publications

References 22 publications

Plasmon-Induced Photoluminescence Immunoassay for Tuberculosis Monitoring Using Gold-Nanoparticle-Decorated Graphene

Plasmon-Induced Photoluminescence Immunoassay for Tuberculosis Monitoring Using Gold-Nanoparticle-Decorated Graphene

Dual-Mode SERS-Fluorescence Immunoassay Using Graphene Quantum Dot Labeling on One-Dimensional Aligned Magnetoplasmonic Nanoparticles

Magneto-actuated immunoassay for the detection of Mycobacterium fortuitum in hemodialysis water

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