Rapid regeneration of plants of Dendrobium lituiflorum Lindl. (Orchidaceae) by using banana extract

Vyas, Shivani; Guha, Satyakam; Bhattacharya, Minakshi; Rao, I. Usha

doi:10.1016/j.scienta.2009.01.012

Cited by 31 publications

(16 citation statements)

References 13 publications

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“…Cell division-inducing compounds present in banana fruit may be responsible for its enhancing effect on orchid embryo development and differentiation. Vyas et al (2009) also observed that the addition of banana homogenate in Knudson's C medium resulted in faster differentiation of Dendrobium lituiflorum Lindl. to form leaves and roots than in control media.…”

Section: Induction Of Roots and Leaves From Dendrobium 'Iriana Jokowimentioning

confidence: 80%

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Handling and propagation of Dendrobium ‘Iriana Jokowi’ in Bogor Botanic Gardens, Indonesia

Rahayu

2016

Nusantara Biosci

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Abstract. Rahayu EMD. 2016. Handling and propagation of Dendrobium 'Iriana Jokowi' in Bogor Botanic Gardens, Indonesia. Nusantara Bioscience 8: 258-263. Dendrobium 'Iriana Jokowi' is a new hybrid in Indonesia which has not being mass propagated yet. Mass propagation is necessary in order to ensure seedling availability. The orchid has been grown in the Orchids Greenhouse of Bogor Botanic Gardens since July 30 th 2015. Handling and care for Dendrobium 'Iriana Jokowi', includes watering, provision of fertilizer, fungicides, and insecticides. Propagation has already been done is using seed culture. The seeds of Dendrobium 'Iriana Jokowi' were sown in four type of culture media, which are modified Vacin & Went (VW), modified Hyponex (HS), modified Knudson's C (KC), and Knudson's C (KCA). The seeds were able to germinate in all four tested media. The highest germination rate in the first 3 months after sowing (3 MAS) were found in KCA media, which are 93.21%. Germinated seed were then subcultured to four type of media in order to induce the growth of leaves and roots. The media used were T1A, T1V, HS, and KCA. After 5 months of planting (5 MAP), result showed that all four media were able to provide significant effect to the number of leaves and roots. The highest number of average leaves grown was 4.8 and found in T1A media. On the other hand, the highest number of average of roots grown was found in T1V media (4,2 roots).

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Section: Induction Of Roots and Leaves From Dendrobium 'Iriana Jokowimentioning

confidence: 80%

“…According to Vyas et al (2009), banana homogenate is known to promote growth of orchid seedlings. It contains minerals, IAA, GA 7 , zeatin, zeatin riboside and 2iP.…”

Section: Induction Of Roots and Leaves From Dendrobium 'Iriana Jokowimentioning

confidence: 99%

Handling and propagation of Dendrobium ‘Iriana Jokowi’ in Bogor Botanic Gardens, Indonesia

Rahayu

2016

Nusantara Biosci

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“…Vyas et al (2009) verificaron que la pulpa de banana (12,5%) en el cultivo de brotes de Dendrobium lituiflorum Lindl. induce a la multiplicación y alargamiento de raíces.…”

Section: Discussionunclassified

“…Tradicionalmente la adición de auxinas y citoquininas ha sido utilizada para regular el crecimiento y desarrollo de tejidos vegetales. No obstante, el uso de estos compuestos es costoso, por tanto se han investigado sustitutos de origen natural que puedan ser una fuente de minerales, aminoácidos, vitaminas y azúcares (Vyas et al, 2009). De acuerdo con Arditti et al (1982), la pulpa de banana puede mejorar el crecimiento de raíces y brotes de orquídea.…”

Section: Introductionunclassified

Suplementos orgánicos para el cultivo in vitro del híbrido Laeliocattleya (Orchidaceae)

Gonçalves¹,

Machado

Ballesta³

et al. 2016

Idesia

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RESUMENEl desarrollo de técnicas de micropropagación en orquídeas ofrece múltiples ventajas como la reproducción de genotipos superiores, raros, estériles o libres de enfermedades. El objetivo del presente trabajo fue evaluar el uso de suplementos orgánicos (agua de coco y pulpa de banana) en el crecimiento y desarrollo in vitro de plántulas del híbrido intergenérico Laeliocattleya (Hadrolaelia purpurata x Cattleya intermedia), comparando su respuesta con fitorreguladores de auxinas y citoquininas. Para ello se realizaron los siguientes cuatro experimentos: 1. Suplementos orgánicos comparados con diferentes concentraciones de kinetina (N6-furfuril amino purina; KIN) y ácido naftalenacético (ANA), 2. Suplementos orgánicos comparados con diferentes concentraciones de ácido 3-indolbutírico (IBA) y N6-benzyladenine (6BA), 3. Suplementos orgánicos comparados con diferentes concentraciones de IBA y KIN, y 4. Suplementos orgánicos comparados con diferentes concentraciones de ANA y 6BA. El suplemento orgánico de agua de coco fue estadísticamente superior al resto (P<0,01), para la variable número de hojas, en todos los experimentos realizados, con 7,3 hojas por plántula cultivada in vitro. En tanto que la adición de pulpa de banana mostró los mejores resultados en el número de raíces (P<0,01), para todos los experimentos en estudio, con un valor medio de 7,4 raíces. Se concluye que la adición de pulpa de banana o de agua de coco es conveniente para la producción de orquídeas híbridas por presentar un efecto positivo en el desarrollo de las plántulas, y por su costo reducido en relación con el uso de reguladores de crecimiento.Palabras clave: pulpa de banana, agua de coco, fitohormonas, auxina, citoquinina. ABSTRACT The development of micropropagation techniques in orchid has

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“…Choice of explant Bhaskaran et al [7] is also an important factor and in general, scutellum derived embryogenic callus from the germinating seeds is employed. However, the calli generated have limited totipotency for successful regeneration [8,9] which further depends on a number of biophysical characteristics including osmotic regulation [10]. Sugars like sucrose and maltose [11] and sugar alcohols like sorbitol and mannitol [12] are involved in maintaining osmotic requirements for the dividing cells.…”

Section: Introductionmentioning

confidence: 99%

Regeneration Enhancement in Tissue Culture of Indica Rice’s through Partial Desiccation and Chemical Supplements

Repalli¹,

Geda²,

Nsn³

et al. 2017

J Plant Biochem Physiol

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Rapid regeneration of plants of Dendrobium lituiflorum Lindl. (Orchidaceae) by using banana extract

Cited by 31 publications

References 13 publications

Handling and propagation of Dendrobium ‘Iriana Jokowi’ in Bogor Botanic Gardens, Indonesia

Handling and propagation of Dendrobium ‘Iriana Jokowi’ in Bogor Botanic Gardens, Indonesia

Suplementos orgánicos para el cultivo in vitro del híbrido Laeliocattleya (Orchidaceae)

Regeneration Enhancement in Tissue Culture of Indica Rice’s through Partial Desiccation and Chemical Supplements

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