Rapid sample preparation for detection of antibiotic resistance on a microfluidic disc platform

Perebikovsky, Alexandra; Liu, Yujia; Hwu, Alexander; Kido, Horacio; Shamloo, Ehsan; Song, Dian; Monti, G; Shoval, Oren R.; Gussin, Daniel J; Madou, Marc

doi:10.1039/d0lc00838a

Cited by 16 publications

(9 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Less expensive and faster point-of-care diagnostic platforms have gained new importance in an age of mass testing prompted by the COVID pandemic [ 11 ]. The demonstrated integrated sample preparation approach can be used in other applications, such as the detection of antibiotic resistance shown in previous work by Perebikovsky et al [ 20 ].…”

Section: Resultsmentioning

confidence: 98%

Integrating Bio-Sensing Array with Blood Plasma Separation on a Centrifugal Platform

Peshin

Madou

Kulinsky

2023

Sensors

Self Cite

View full text Add to dashboard Cite

Numerous immunoassays have been successfully integrated on disc-based centrifugal platforms (CDs) over the last 20 years. These CD devices can be used as portable point-of-care (POC) platforms with sample-to-answer capabilities where bodily fluids such as whole blood can be used as samples directly without pre-processing. In order to use whole blood as a sample on CDs, centrifugation is used to separate red blood cells from plasma on CDs. There are several techniques for using specific fluidic patterns in the centrifugal fluidic network, such as reciprocation, that enhances the sensitivity of the immunoassays, including those using microarray antigen membranes. Present work demonstrates, for the first time, simultaneous integration of blood plasma separation (BPS) and reciprocation on the CD platform. The integrated design allows plasma that is separated from the red blood cells in a sedimentation chamber to flow into the reciprocation chamber via a narrow connecting channel of 0.5 mm × 0.5 mm cross-section. Due to the small cross-section of the connecting channel, there is no inflow of the red blood cell into the reciprocation chamber during subsequent fluidic operations of the CD. While no inflow of the red blood cells into the reciprocation chamber was observed, the conditions of 20 g jerk acceleration were also simulated in ANSYS finite element analysis software, and it was found that the CD design that was used is capable of retaining red blood cells in the sedimentation chamber. Experimentally, the isolation of red blood cells in the sedimentation chamber was confirmed using the ImageJ image processor to detect the visible color-based separation of the plasma from the blood. A fluorescent analyte testing on the bio-sensing array of the presented novel integrated design and on the standard reciprocation design CD was conducted for 7 min of reciprocation in each case. The test analyte was Europium Streptavidin Polystyrene analyte (10−3 mg/mL) and the microarray consisted of Biotin bovine serum albumin (BSA) dots. The fluorescent signals for the standard and integrated designs were nearly identical (within the margin of error) for the first several minutes of reciprocation, but the fluorescent signal for the integrated design was significantly higher when the reciprocation time was increased to 7 min.

show abstract

Section: Resultsmentioning

confidence: 98%

Integrating Bio-Sensing Array with Blood Plasma Separation on a Centrifugal Platform

Peshin

Madou

Kulinsky

2023

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…The heating was achieved by IR lamps in the CD Driver mounted below the disc and was modulated by the IR sensor mounted directly above the CD reaction chamber H in the lid of CD Driver. A brief description of CD Driver is presented in Section 2.5 and more details can be found in our previous report ( Perebikovsky et al, 2021 ). The total time from sample loading to final results is approximate 45 min.…”

Section: Methodsmentioning

confidence: 99%

“…It consists of several major subsystems that control the disc rotation speed and duration, the temperature in the amplification chambers, and the digital image capture and processing. The core details of disc driver are shown in a recent paper from our group ( Perebikovsky et al, 2021 ). The CD Driver was modified for on-disc nucleic acid amplification with the installation of infrared (IR) lamps and IR sensors for temperature control.…”

Section: Methodsmentioning

confidence: 99%

Development of a proof-of-concept microfluidic portable pathogen analysis system for water quality monitoring

Gowda

Kido

et al. 2022

Science of The Total Environment

Self Cite

View full text Add to dashboard Cite

show abstract

“…ECM mainly contains collagen, elastin, glycoproteins, and polysaccharides (Dutta and Dutta, 2009). In the past decades, many natural biomaterials [e.g., gelatin hydrogel (GA), hyaluronic acid (HA), and matrigel] have been used for 3D cell-culture in vitro (Li et al, 2019;Perebikovsky et al, 2021). GA is a subtype of collagen, which can be isolated from bones, ligaments, and tendons.…”

Section: Introductionmentioning

confidence: 99%

Microfluidics-Based Single-Cell Research for Intercellular Interaction

Pang

Ding

Liu

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Intercellular interaction between cell–cell and cell–ECM is critical to numerous biology and medical studies, such as stem cell differentiation, immunotherapy and tissue engineering. Traditional methods employed for delving into intercellular interaction are limited by expensive equipment and sophisticated procedures. Microfluidics technique is considered as one of the powerful measures capable of precisely capturing and manipulating cells and achieving low reagent consumption and high throughput with decidedly integrated functional components. Over the past few years, microfluidics-based systems for intercellular interaction study at a single-cell level have become frequently adopted. This review focuses on microfluidic single-cell studies for intercellular interaction in a 2D or 3D environment with a variety of cell manipulating techniques and applications. The challenges to be overcome are highlighted.

show abstract

Rapid sample preparation for detection of antibiotic resistance on a microfluidic disc platform

Abstract: We demonstrated a novel instrument and a centrifugal microfluidics disc design that is capable of remarkably accomplishing the sample treatment steps, which pave the way to realize the antibody susceptibility test in point-of-care situation.

Cited by 16 publications

References 18 publications

Integrating Bio-Sensing Array with Blood Plasma Separation on a Centrifugal Platform

Integrating Bio-Sensing Array with Blood Plasma Separation on a Centrifugal Platform

Development of a proof-of-concept microfluidic portable pathogen analysis system for water quality monitoring

Microfluidics-Based Single-Cell Research for Intercellular Interaction

Contact Info

Product

Resources

About