Rapid separation of triterpenoids from Neem seed extracts

Jarvis, Andrew P.; Morgan, E. David; Edwards, Christine

doi:10.1002/(sici)1099-1565(199901/02)10:1<39::aid-pca426>3.0.co;2-l

Cited by 37 publications

(34 citation statements)

References 17 publications

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“…Under the present experimental conditions, the hexane extract was also apparently not toxic to J2. The toxicity of the methanolic extracts is in agreement with other literature studies where tetranortriterpenoids were reported in neem seed and activity related to several pathogens (Govindachari et al, 1990(Govindachari et al, , 1998Jarvis et al, 1999;Johnson & Morgan, 1997a, b;Martinez, 2002;Sharma et al, 2003;Barrek et al, 2004).…”

Section: Discussionsupporting

confidence: 90%

Effect of neem seed extracts on the development of the Soybean Cysts Nematode

Silva¹,

Oliveira²,

Jham³

et al. 2008

Trop. plant pathol.

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The effect produced by neem seed extracts and a neem seed concentrate (NSC) on the mortality and development of Heterodera glycines was evaluated. Second-stage juvenile (J2) mortality produced by the extracts and NSC was determined. Soybean seedlings treated with each of the extracts (aqueous, methanolic and hexane) were inoculated with J2 and the number of females and eggs per root system determined after 30 days. Both the hexane extracts were ineffective as no statistical difference (P>0.05) in J2 mortality was observed between them and the control. No statistical difference (P>0.05) in J2 mortality (>98%) was obtained between the water extracts and methanol extract at 1000 mg L -1 treatments. The J2 percentage of mortality exponentially increased with the NSC dosages (R 2 =0.94). LD 50 was 42.6 mg L -1 for NSC on H. glycines. In the greenhouse experiment, the highest reduction in the number of females was 84%, obtained with the aqueous (41.6 mg L -1) and methanolic extracts (1000 mg L -1 ). In the same way, the highest reduction in the number of eggs, of about 90%, was obtained with the same extracts. Thus, using aqueous extracts is more suitable than the remaining extracts in field conditions. The activities of the methanol and NSC extracts were attributed to the seven tetranortritepenoids (azadirachtin H, azadirachtin A, azadirachtin B, desacetylnimbin, desacetylsalannin, nimbin and salannin) identified by reverse phase high performance liquid chromatography. Keywords: Azadirachta indica, Heterodera glycines, tetranortriterpenoids, control. RESUMO Efeito dos extratos de sementes de Nim no desenvolvimento de Heterodera glycinesO efeito dos extratos de sementes de nim e do extrato de sementes concentrado (NSC) foi avaliado na mortalidade e desenvolvimento de Heterodera glycines. A mortalidade de juvenis de segundo estádio (J2) foi avaliada na presença dos extratos de sementes (aquosos, metanólicos e hexânicos) e NCS in vitro. Em casa de vegetação, o número de fêmeas e de ovos por sistema radicular foi determinado 30 dias após a inoculação de plantas de soja com J2 tratados com os extratos de sementes. Os extratos hexânicos foram ineficientes em matar os J2, conquanto nenhuma diferença estatística (P>0.05) foi observada entre as doses estudadas e a testemunha. Os extratos aquosos e o metanólico a 1000 mg L -1 causaram a morte de pelo menos 98% dos J2, mas não houve diferença significativa entre estes tratamentos. A mortalidade dos J2 tratados com NSC cresceu exponencialmente com o aumento das doses (R 2 =0.94). O LD 50 do NSC sobre J2 de H. glycines foi de 42.6 mg L -1 . Em casa de vegetação, as maiores reduções no número de fêmeas, 84%, foram obtidas com o extrato aquoso (41.6 mg L -1 ) e metanólico (1000 mg L -1 ). Da mesma forma, as maiores reduções no número de ovos, cerca de 90%, foram obtidas com os mesmos extratos. Considerou-se que o uso do extrato aquoso em condições de campo é mais apropriado que os demais extratos. As atividades do extrato metanólico e do NSC foram atribuídas a sete tetranor...

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Section: Discussionsupporting

confidence: 90%

Effect of neem seed extracts on the development of the Soybean Cysts Nematode

Silva¹,

Oliveira²,

Jham³

et al. 2008

Trop. plant pathol.

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“…This capacity may have been possible due to the presence in the extract of compounds such as tetratriterpenoids, including azadirachtin (Jarvis et al, 1999). These compounds may be able to inhibit the S. aureus production polysaccharide intercellular adhesin due inhibition of ica genes expression (Mack et al, 2004;Atshan et al, 2012) or could have acted on S. aureus cell wall hydrophobicity (Kustos et al, 2003).…”

Section: Effect Of Neem Ee On S Aureusmentioning

confidence: 99%

Effect of neem ( Azadirachta indica A. Juss) leaf extract on resistant Staphylococcus aureus biofilm formation and Schistosoma mansoni worms

Quelemes

Perfeito

Guimarães

et al. 2015

Journal of Ethnopharmacology

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“…Una parte de este extracto se rotuló y se guardó hasta los bioensayos de actividad antifúngica. Se realizó un procedimiento de fraccionamiento de la otra parte del extracto crudo, por cromatografía en columna, con base en la información de Jarvis et al (1999). Se inició con 2,3 g de extracto crudo de biomasa celular, el cual se mezcló homogéneamente con 30 g de silica gel de 63 -200 micrones (Panreac) para conformar la cabeza de la columna.…”

Section: Obtención De Extractosunclassified

Actividad antifúngica de extractos de biomasa celular de neem sobre aislamientos de dermatofitos

Salazar¹,

Sánchez²,

Arteaga³

et al. 2014

Rev. Colomb. Biotecnol.

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ResumenLas líneas celulares de neem (Azadirachta indica A. Juss.) cultivadas en suspensión líquida han demostrado producir metabolitos secundarios bioactivos, particularmente triterpenoides. En consecuencia, se han realizado estudios para el control de microorganismos de importancia médica, como los hongos dermatofitos. El objetivo principal de este trabajo fue evaluar a través de un método de referencia in vitro la actividad antifúngica de diferentes extractos de cultivos celulares de neem sobre varios aislamientos de Trichophyton mentagrophytes, Trichophyton rubrum y Epidermophyton floccosum. Se realizó un escalado de cultivos de suspensiones celulares de neem, a partir de los cuales se obtuvo un extracto crudo metanólico. Éste extracto fue fraccionado posteriormente por cromatografía en columna de silica gel. Con los extractos obtenidos se determinó la Concentración Mínima Inhibitoria (CMI), siguiendo el método de microdilución en caldo M38-A2, con cinco aislamientos de T. mentagrophytes, cinco de T. rubrum y tres de E. floccosum. Se usó como control positivo el antimicótico Terbinafina. Los resultados mostraron que el extracto crudo de biomasa celular de neem inhibe el crecimiento hasta en 100 % de T. mentagrophytes, T. rubrum y E. floccosum. Al evaluar las fracciones por separado, se observó que las de menor polaridad exhibieron en general mayor actividad antifúngica (CMI=109 µg/mL) que el extracto crudo per se (CMI=2500 µg/ mL) y las fracciones más polares (CMI=7000 µg/mL). Lo anterior indica que las células de neem cultivadas en suspensión producen compuestos con actividad antifúngica, siendo más bioactivos los presentes en las fracciones de menor polaridad.Palabras clave: método de microdilución en caldo, cromatografía, cultivo de células en suspensión, concentración mínima inhibitoria, compuestos bioactivos. AbstractCell lines of neem (Azadirachta indica A. Juss.) grown in liquid suspension have shown to produce bioactive secondary metabolites, particularly triterpenoids. In consequence, its use as a control of medical microorganisms (like dermatophytes) is proposed. The main goal of this study was to assess the antifungal activity of methanolic extracts from neem cultured cell suspensions on several isolates of Trichophyton mentagrophytes (five isolates), Trichophyton rubrum (five isolates) and Epidermophyton floccosum (three isolates). Neem cell suspension cultures were scaled up, from which a raw methanolic extract was obtained. This extract was fractionated by silica gel column chromatography. The raw methanolic extract and its fractions were used in order to determine the Minimal Inhibitory Concentration (MIC) on the dermatophytes isolates by following M38-A2 broth microdilution method. Antimycotic Terbinafine was used as positive control. The results shown that neem raw cellular biomass extract inhibits the growth of T. mentagrophytes, T. rubrum and E. floccosum in at least 100%. In the evaluations of the separated fractions, it was observed that the low polarity fractions had higher antifungal ac...

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Rapid separation of triterpenoids from Neem seed extracts

Cited by 37 publications

References 17 publications

Effect of neem seed extracts on the development of the Soybean Cysts Nematode

Effect of neem seed extracts on the development of the Soybean Cysts Nematode

Effect of neem ( Azadirachta indica A. Juss) leaf extract on resistant Staphylococcus aureus biofilm formation and Schistosoma mansoni worms

Actividad antifúngica de extractos de biomasa celular de neem sobre aislamientos de dermatofitos

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