Rapid Two-Dimensional Procedure

Rockland, Louis B.; Underwood, J. C.

doi:10.1021/ac60094a008

Cited by 44 publications

(11 citation statements)

References 21 publications

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“…1. A,, Yal0~7, K. S., Schreiber, S. S., 6 U'ith the proposal that selenium be included among the essential dietary elements ( 1 1. interest has been focused oa many unanswered questions about the metabolism and fate of selenium in the mammalian organism. 2.…”

Section: Selenium-75 I N D O G Hairmentioning

confidence: 99%

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Incorporation of Selenium-75 into Dog Hair

McConnell

Kreamer

1960

Experimental Biology and Medicine

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Section: Selenium-75 I N D O G Hairmentioning

confidence: 99%

“…Two solvent systems were employed: a teyt-butyl alcohd-formic acid-water mixture (Solvent I) ; and a single phase phenol-ammonia-water mixture (Solvent 11) (6). The protein was then washed repeatedly with water, lyophilized.…”

Section: Selenium-75 I N D O G Hairmentioning

confidence: 99%

Incorporation of Selenium-75 into Dog Hair

McConnell

Kreamer

1960

Experimental Biology and Medicine

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“…The water-soluble substances were concentrated by freeze-drying and separated by descending two-directional paper chromatography. Tertiary butanol-formic acid-water (695 : 10: 295) was used as the first solvent and phenol-water-ammonia (155 : 43 : 2, W/V/V) as the second ( ROCKLAND and UNDERWOOD, 1954).…”

Section: Chromatographymentioning

confidence: 99%

Pathways of Translocation and Metabolic Conversions of Root-Absorbed 14c (U) L-Glutamic Acid in Tomato Plants

Die¹

1963

Acta Botanica Neerlandica

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14C (U) L‐glutamic acid was supplied to the roots of young tomato plants. After a four hours' uptake period all the amino acids present in the root proteins were weakly labelled, but of the free amino acids of the roots only γ‐aminobutyric acid, glutamine, glutamic acid and aspartic acid proved to be active. In addition, the label was incorporated into lipoids and a number of carboxylic acids. The extent of labelling of the various leaves appeared to be correlated with the synthetic activities of these leaves, and inversely related with leaf age. The distribution was not influenced by the presence or absence of roots. It is concluded that the xylem is the initial way of translocation of amino acids, which are synthesized in the roots. During their movement through the xylem vessels to the areas of transpiration, the amino acids are partly absorbed by surrounding tissues and translocated to centres of protein synthesis.

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“…Sugars were separated on small paper chromatograms (Rockland & Underwood, 1954) with ethyl acetatepyridine-water (2:1:1, by vol., top layer) as developing solvent (Jermyn & Isherwood, 1949). The position of the sugar spots was revealed by spraying with the AgNO3 reagent of Trevelyan, Procter & Harrison (1950).…”

mentioning

confidence: 99%

Identification of guanosine diphosphate derivatives of d-xylose, d-glucose and d-galactose in mature strawberry leaves

Selvendran

Isherwood

1967

Biochemical Journal

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The nucleotides from a trichloroacetic acid extract of mature strawberry leaves were separated into ten main fractions by chromatography on a Dowex 1 (formate form) column with ammonium formate as the eluting agent. One of these fractions, which was suspected to contain not only ADP but also GDP-sugars, was separated into a number of subfractions by further chromatography on a Dowex 1 (formate form) column with the formic acid system as the eluting agent. One of these subfractions was identified from its ultraviolet spectra, from its position on the two ion-exchange columns and by thin-layer chromatography as a GDP-sugar. Mild acid hydrolysis gave GDP and a mixture of sugars. The sugars, after a preliminary separation on a paper chromatogram, were identified by an isotope-dilution method. The sugars were condensed with sodium [(14)C]cyanide, the [(14)C]nitriles were hydrolysed and one of the epimeric acids was isolated, either as lactone or amide, by co-crystallization with a non-radioactive carrier. This method distinguishes between enantiomorphic sugars. d-Mannose, d-xylose, d-glucose and d-galactose were present in the proportions 40:10:1:1 respectively. The total amount of the GDP-sugars was approx. 0.1mumole/100g. of fresh leaves.

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Rapid Two-Dimensional Procedure

Cited by 44 publications

References 21 publications

Incorporation of Selenium-75 into Dog Hair

Incorporation of Selenium-75 into Dog Hair

Pathways of Translocation and Metabolic Conversions of Root-Absorbed 14c (U) L-Glutamic Acid in Tomato Plants

Identification of guanosine diphosphate derivatives of d-xylose, d-glucose and d-galactose in mature strawberry leaves

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