Rat Sertoli cells acquire a beta-adrenergic response during primary culture.

Kierszenbaum, Abraham L.; Spruill, W A; White, Myra; Tres, Laura L.; Perkins, John P.

doi:10.1073/pnas.82.7.2049

Cited by 26 publications

(8 citation statements)

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“…Such an acquisition of the response to ß-adrenergic agonist has been reported for different cell types such as hepatocytes (Nakamura et al, 1983), granulosa cells (Kliachko & Zor, 1981) and Sertoli cells (Kierszenbaum et al, 1985). As shown in the current study, ß-agonists did not affect or only slightly increased testosterone production in fresh interstitial cells derived from adult testis (Cooke et al, 1982;Moger et al, 1982).…”

Section: Discussionsupporting

confidence: 85%

Catecholamine-induced stimulation of testosterone production by Leydig cells from fetal mouse testis

Pointis¹,

Latreille²

1987

Reproduction

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Summary. In contrast to the strong stimulation of testosterone production by hCG, L-isoproterenol had little effect on freshly isolated Leydig cells from 18-day-old mouse fetuses. However, the ability of fetal Leydig cells to respond to L-isoproterenol exposure increased during culture (0\p=n-\24h). The response of the cultured cells to Lisoproterenol was dose-dependent with an ED50 at 2 \ m=x\10\m=-\7M. Adrenaline and noradrenaline at a concentration of 10 \ m=-\ 5 M also increased testosterone production by cultured fetal Leydig cells. DL-Propranolol, a \g=b\-antagonist,inhibited L-isoproterenolstimulated testosterone production in a dose-dependent manner, while phentolamine, an \g=a\-adrenergicantagonist, had no effect. These results suggest that catecholamines may play an essential role in the control of testicular steroidogenesis during fetal development.

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Section: Discussionsupporting

confidence: 85%

Catecholamine-induced stimulation of testosterone production by Leydig cells from fetal mouse testis

Pointis¹,

Latreille²

1987

Reproduction

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“…Under physiological conditions, most vimentin in cells is present in intermediate filaments (Klymkowsky et al, 1989;Darnell et al, 1990). In most cultured and well-spread cells, including rat Sertoli cells, vimentin IFs are shown to be a delicate network of thread-like filaments surrounding the nuclei and extending through the cytoplasm to the plasma membrane (Spruill et al, 1983; Kierszenbaum et al, 1985;Alberts et al, 1989). In the present in vivo study, vimentin in rat Sertoli cells was also shown to be concentrated around the nucleus, extending well into the cytoplasm and connecting to many specialized structures on the cell membrane.…”

Section: Distribution Of Ifs In Rat Sertoli Cellsmentioning

confidence: 99%

Changes in the distribution of intermediate filaments in rat Sertoli cells during the seminiferous epithelium cycle and postnatal development

et al. 1997

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Background Intermediate filaments (IFs) are components of the cytoskeleton. In mammalian Sertoli cell, IFs are formed by vimentin. Previous studies have shown some characteristics of its distribution in Sertoli cells, however, very little is known of its distributional changes during the seminiferous epithelium cycle and during postnatal development. Methods: Immunohistochemical and electron microscopic methods were used to determine the distribution of vimentin‐type IFs in rat Sertoli cells during the seminiferous epithelium cycle and postnatal development. Results: The distribution of IFs in adult rat Sertoli cell showed distinct cyclic changes during the seminiferous epithelium cycle. At stages I‐VI, bundles of IFs extend from the perinuclear region to the supranuclear and apical regions of the Sertoli cell. These apical extensions became shorter at stage VII, and at stages VIII‐X IFs were observed only in the perinuclear region. Short apical extensions reappeared at stages XI‐XII; and at stages XIII‐XIV, they extended again into the apical region. During this cycle, IFs were always closely associated with the heads of elongate spermatids. IFs were also shown to be in close apposition to some specialized structures on the cell membrane, such as the ectoplasmic specialization between adjacent Sertoli cells. During postnatal (p.n.) development, IFs were mainly observed at the basal nuclear region on p.n. day 7. The IFs in the supranuclear or apical regions first appeared at p.n. day 14 and gradually increased during the development. The perinuclear IFs network was fully established by p.n. day 28 and the adult distribution pattern of the IFs was established by p.n. day 42. Conclusions: Vimentin‐type IFs in rat Sertoli cells are a delicate endocellular network, which is centered in the perinuclear region and extends to the apical region of the cell. During the seminiferous epithelium cycle, the distribution of IFs changes in a stage‐dependent manner and is closely related to the location of the heads of elongate spermatids. During postnatal development, IFs gradually increase in numbers and the main distribution area is transferred from the basal nuclear to the perinuclear and supranuclear regions. Anat. Rec. 248:391‐405, 1997. © 1997 Wiley‐Liss, Inc.

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“…Leydig cells have beta-adrenergic receptors and catecholamines stimulate adenylate cyclase and steroidogenesis in cultured Leydig cells (Cooke et al -1982;Moger et al -1982, Damber andSelstam -1983). Beta adrenergic receptors are also present on Sertoli cells in culture and isoproterenol mimics several of the effects of FSH on cultured Sertoli cells (Verhoven et al -1979;Heindel et al -1981), whereas, in vivo the tubules probably do not contain beta adrenoreceptors (Kierszenbaum et al -1985).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Long-Term Variation in Sympathetic Activity on Testicular Morphology in Immature Rats

et al. 2009

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In order to investigate the effect of increased and decreased sympathetic activity on testicular development, immature male rats were injected with isoproterenol or 6-hydroxydopamine from birth up to 49 days of age. Isoproterenol treatment resulted in a marked Leydig cell hypertrophy but the development of the spermatogenic epithelium was unaffected compared to that in saline treated controls. 6-hydroxydopamine treatment in a dose inhibiting the development of the parotid gland did not influence testicular development. Effekt von langfristiger Schwankung der Sympathikusaktivitat auf die Hodenmorphologie bei unreifen RattenZusammenfassung: Zur Untersuchung der Wirkung von herabgesetzter und gesteigerter Sympathikusaktivitat auf die Hodenentwicklung wurde unreifen mannlichen Ratten von der Geburt bis zum 49. Lebenstage Isoproterenol oder 6-Hydroxydopamin injiziert. Die Behandlung mit Isoproterenol hat eine deutliche Leydigzellhypertrophie zur Folge, die Entwicklung des Keimepithels wurde, verglichen mit den Kochsalz-behandelten Kontrollen, nicht beeinfludt. 6-Hydroxydopamin, in einer, die Entwicklung der Glandula parotis hemmenden Dosis, beeinfludte die Entwicklung des Hodens nicht.

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Rat Sertoli cells acquire a beta-adrenergic response during primary culture.

Abstract: Two-dimensional polyacrylamide gel electrophoresis and the radioligand (-)- [25I]iodopindolol have been used to study isoproterenol-dependent protein phosphorylation and /3-adrenergic receptor availability, respective-

Cited by 26 publications

References 23 publications

Catecholamine-induced stimulation of testosterone production by Leydig cells from fetal mouse testis

Catecholamine-induced stimulation of testosterone production by Leydig cells from fetal mouse testis

Changes in the distribution of intermediate filaments in rat Sertoli cells during the seminiferous epithelium cycle and postnatal development

The Effect of Long-Term Variation in Sympathetic Activity on Testicular Morphology in Immature Rats

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