RATIO PENGENCERAN SPERMA TERHADAP MOTILITAS SPERMATOZOA, FERTILITAS DAN DAYA TETAS IKAN LELE (Clarias sp.)

Abstract

This study was aimed to determine the effect of the dilution ratio of sperm with optimal NaCl and fructose on the motility of the catfish, Clarias sp., spermatozoa, fertility and hatchability of eggs. Catfish used in this study consisted of one parent pairs (male and female weight of 1000 grams weight of 1500 g). NaCl and fructose solution were diluted with aquabidest. The observation was conducted on the motility of spermatozoa, fertility and hatchability of eggs. The experimental design used completely randomized design (CRD). Dilution ratio is 1: 0, 1, 20, 1: 40, 1; 60, 1: 80, and 1: 100 with replicated 3 times. Observations were carried out soon after the sperm mixed with diluents. Fertility occurred 12 hours after fertilization. Egg hatchability was observed after fertilization. The results showed that the ratio of dilution gave significant effect on the sperm motility, fertility and hatchability of the eggs. This research found that the dilution ratio 1: 60 was the best treatment with the average sperm motility of 96. 66%, fertility of 71, 66 5% and egg hatchability of 70%.

Additional of coconut water and glycerol in sperm storage spermatozoa motility and viability of comet fish, Carassius auratus

“…The percentage of sperm motility in the post cryopreservation observation (day 4) decreased compared to before cryopreservation (day 0), this was due to the limited availability of nutrients used as energy source in the diluent. According to Adipu et al [14]. stated that the decrease in motility leads to a weakness in the ability to fertilize the egg.…”

Penggunaan Media Perendaman dari Daun Kelor (Moringa oleifera L.) dengan Konsentrasi yang Berbeda Terhadap Daya Rekat dan Daya Tetas Ikan Lele Mutiara (Clarias gariepinus)

“…Selain faktor lingkungan, penyebab kematian telur ikan lele juga diakibatkan sifat adhesif dari telur ikan lele tesebut. Adipu et al, (2011) menambahkan, faktor internal yang mempengaruhi daya tetas telur ikan adalah perkembangan embrio yang terhambat. Hal ini dikarenakan kualitas spermatozoa dan telur ikan tersebut.…”

Pengaruh Pemberian Jagung Kuning Fermentasi Dengan Suplementasi Polyunsaturated Fatty Acid (Pufa) Dan L-Karnitin Terhadap Daya Tetas Telur Puyuh

“…Salah satu penentu fertilitas adalah motilitas sperma atau pergerakan sperma. Adipu et al (2011) menyatakan bahwa sperma memerlukan energi karena dalam proses fertilisasi sperma menggunakannya untuk bergerak menuju ovum sehingga dapat membuahi ovum. Hal ini dimungkinkan pengaturan energi untuk motilitas sperma dipengaruhi oleh kandungan laktosa dalam cairan sperma.…”