Rational design and bioimaging application of cholesterol conjugated fluorescence probe for Cu2+ detection

Zhong, Chunli; Qiu, Jian‐Wen; Li, Meng; Yuan, Yaofeng; Zhu, Hongmin; Gao, Yong

doi:10.1016/j.jphotochem.2021.113267

Cited by 11 publications

(3 citation statements)

References 48 publications

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“…[42][43][44][45] As shown in Scheme 2, unique structure of P1 and different physical and chemical properties of Cu 2+ and Fe 3+ endow the probe P1 the ability to recognize ions through coordination. N and O atoms could transfer their lone electron pairs to the unoccupied orbits of ions [42,[46][47][48][49][50][51] to form seven-membered ring complexes. Different physical and chemical properties of Cu 2+ and Fe 3+ lead to different dissociation abilities of ligands.…”

Section: Design Of P1mentioning

confidence: 99%

Lysosome‐Targeting Polysiloxane Fluorescent Probe for Continuous Detection of Fe³⁺, Cu²⁺, and H₂O₂

Zhang

Ding

Gai

et al. 2022

Macro Chemistry & Physics

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In this study, a simple and nontoxic polysiloxane fluorescent probe (P1) is synthesized to detect iron ions, copper ions, and hydrogen peroxide (H 2 O 2 ). Interestingly, both Fe 3+ and Cu 2+ can gradually quench the fluorescence intensity of the probe, and H 2 O 2 can reproduce the probe quenched by Cu 2+ instead of Fe 3+ . The probe shows high selectivity and photostability, and the fluorescence intensity can keep at 95.77% after irradiation at 260 nm for 40 min. In addition, P1 can stably locate lysosome. With excellent property, the probe as lysosome tracker has broad application prospects in detection and biological imaging.

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Section: Design Of P1mentioning

confidence: 99%

Lysosome‐Targeting Polysiloxane Fluorescent Probe for Continuous Detection of Fe³⁺, Cu²⁺, and H₂O₂

Zhang

Ding

Gai

et al. 2022

Macro Chemistry & Physics

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“…Copper is the third most abundant heavy metal (after iron and zinc) [1][2][3] and it is widely distributed in various tissues and plays a vital function in a variety of physiological processes in the human body [4][5][6], such as biological metabolism, enzyme catalysis, biomineralization, regulation of osmotic pressure and signaling [7][8][9][10][11][12]. Excessive Cu 2+ can result in neurodegenerative diseases like Alzheimer's and Parkinson's, as well as other metabolic abnormalities including hypoglycemia [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

A novel reversible fluorescent probe for Cu²⁺ and S²⁻ ions and imaging in living cells

Cheng

Sun

et al. 2022

Methods Appl. Fluoresc.

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A novel fluorescent probe TSOC (thiazole salicylaldehyde oxazole chlorinated) was synthesized based on benzothiazole conjugated olefinic bond with salicylicaldehyde unit as fluorophore and a phenyl oxazole unit as bonding unit. The probe could reversibly detect of Cu2+ and S2− over other common ions with longer emission and large stokes shift in an aqueous solution at pH 7.3 (DMSO-Hepes, v/v, 5:1, 10 mM). The bonding mechanism was supported through the titration experiment of fluorescence and absorption spectroscopy, 1H-NMR titration, HR-MS and DFT calculations. Moreover, the probe further exhibited good cell permeability and were successfully used to visualize Cu2+ and S2− in living cells.

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“…The hydrophilic nature of fluorescent dyes greatly contributes to its compatibility with biological systems such as in enzyme detection and cell tracking. Fluorescent sensors have successfully been utilized in imaging cell structures and for analyzing biophysical processes: for example, in the detection of Alzheimer’s disease and the activity of certain enzymes, as well as pH and the concentration of certain metal ions [ 1 , 2 , 3 , 4 ]. Due to its versatility and widespread utility as a fluorescent probe, numerous synthetic routes have been reported on xanthene dyes and fluorescein derivatives [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Rational Design of a Fluorescent Chromophore as a Calcium Receptor via DFT and Multivariate Approaches

et al. 2022

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Computational and experimental approaches were adopted to utilize a chromophore diglycolic functionalized fluorescein derivative as a Ca2+ receptor. Fluorescein diglycolic acid (Fl-DGA, 1) was synthesized and used in multivariate determination of Ca2+ and K+. Full-structure computation shows that the complexes of 1 and Ca2+ have comparable energies regardless of additional interaction with lactone moiety. The initial formation of diglycolic-Ca2+ complex followed by macrocyclization is thermodynamically disfavored. A U-shaped pre-organized 1 allows Ca2+ to interact simultaneously with diglycolic and lactone motifs. Both motifs actively participate in Ca2+ recognition and the eleven methylene units in the undecyl arm provides excellent flexibility for reorganization and optimum interaction. Principal component analysis (PCA) of computational molecular properties reveals a simple method in evaluating motifs for cation recognition. Fragment models support full-structure results that negative charge causes significant structural changes, but do not reproduce the full extent of C-O bond breaking observed in the latter. Experimental optical responses show that 1 is selective towards Ca2+ and discriminates against K+ and Mg2+. PCA of emission intensities affords distinct clusters of 0.01, 0.1 and 1 mM Ca2+ and K+, and suggests applicability of this technique for simultaneous determination of cationic plant macronutrients in precision agriculture and a wide variety of other applications.

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Rational design and bioimaging application of cholesterol conjugated fluorescence probe for Cu2+ detection

Cited by 11 publications

References 48 publications

Lysosome‐Targeting Polysiloxane Fluorescent Probe for Continuous Detection of Fe³⁺, Cu²⁺, and H₂O₂

Lysosome‐Targeting Polysiloxane Fluorescent Probe for Continuous Detection of Fe³⁺, Cu²⁺, and H₂O₂

A novel reversible fluorescent probe for Cu²⁺ and S²⁻ ions and imaging in living cells

Rational Design of a Fluorescent Chromophore as a Calcium Receptor via DFT and Multivariate Approaches

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Rational design and bioimaging application of cholesterol conjugated fluorescence probe for Cu2+ detection

Cited by 11 publications

References 48 publications

Lysosome‐Targeting Polysiloxane Fluorescent Probe for Continuous Detection of Fe3+, Cu2+, and H2O2

Lysosome‐Targeting Polysiloxane Fluorescent Probe for Continuous Detection of Fe3+, Cu2+, and H2O2

A novel reversible fluorescent probe for Cu2+ and S2− ions and imaging in living cells

Rational Design of a Fluorescent Chromophore as a Calcium Receptor via DFT and Multivariate Approaches

Contact Info

Product

Resources

About

Lysosome‐Targeting Polysiloxane Fluorescent Probe for Continuous Detection of Fe³⁺, Cu²⁺, and H₂O₂

Lysosome‐Targeting Polysiloxane Fluorescent Probe for Continuous Detection of Fe³⁺, Cu²⁺, and H₂O₂

A novel reversible fluorescent probe for Cu²⁺ and S²⁻ ions and imaging in living cells