Rational design of additively manufactured Ti6Al4V implants to control Staphylococcus aureus biofilm formation

Sarker, Avik; Tran, Nhiem; Rifai, Aaqil; Brandt, Milan; Tran, Phong A.; Leary, Martin; Fox, Kate; Williams, Richard J.

doi:10.1016/j.mtla.2019.100250

Cited by 52 publications

(38 citation statements)

References 57 publications

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“…In this sense, different additive manufacturing specimens received the addition of antimicrobial agents, such as silver ions or nanoparticles [ [97] , [98] , [99] ], antibiotic drugs [ 30 , 100 ], the addition of copper or silver to the titanium alloy [ 101 , 102 ], ZnO nanoarrays [ 103 ], modifications by calcium phosphate incorporated into TiO 2 nanotubes [ 104 ] and among others polystyrene and acrylic acid solutions [ 105 ]. Sarker et al [ 106 ], fabricated additive manufacturing specimens with tilts and observed a reduction in S. aureus biofilm formation, and these results were associated with changes in surface topography, such as wettability and roughness.…”

Section: Strategies For Interrupting Biofilm Formationmentioning

confidence: 99%

Bacterial adhesion to biomaterials: What regulates this attachment? A review

Kreve

Reis

2021

Japanese Dental Science Review

128

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Highlights Bacterial adhesion to the surface of dental materials play a significant role in infections. The factors that govern microbial attachment involves different types of physical-chemical interactions and biological processes. Studying bacterial adhesion makes it possible to understand the mechanisms involved in attachment and helps in the search for technologies that promote antibacterial surfaces.

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Section: Strategies For Interrupting Biofilm Formationmentioning

confidence: 99%

Bacterial adhesion to biomaterials: What regulates this attachment? A review

Kreve

Reis

2021

Japanese Dental Science Review

128

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“…To overcome these complications, the chemical and mechanical characteristics of these devices are being tuned accordingly. For example, several coatings have been exploited to reduce the biofilm formation [18][19][20] and increase the life expectancy of the implants [21][22][23], while tailorable mechanical responses can improve the formation of an optimal interaction with the physiological tissue [24][25][26], reducing the stress-shielding effect at the interface that is responsible for most of the failures of these devices. Among these perspectives, the surface composition and morphology are crucial parameters that influence the primary response of the body to an implant depending on the anatomical region [27].…”

Section: Introductionmentioning

confidence: 99%

Selective Laser Melting and Electron Beam Melting of Ti6Al4V for Orthopedic Applications: A Comparative Study on the Applied Building Direction

et al. 2020

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The 3D printing process offers several advantages to the medical industry by producing complex and bespoke devices that accurately reproduce customized patient geometries. Despite the recent developments that strongly enhanced the dominance of additive manufacturing (AM) techniques over conventional methods, processes need to be continually optimized and controlled to obtain implants that can fulfill all the requirements of the surgical procedure and the anatomical district of interest. The best outcomes of an implant derive from optimal compromise and balance between a good interaction with the surrounding tissue through cell attachment and reduced inflammatory response mainly caused by a weak interface with the native tissue or bacteria colonization of the implant surface. For these reasons, the chemical, morphological, and mechanical properties of a device need to be designed in order to assure the best performances considering the in vivo environment components. In particular, complex 3D geometries can be produced with high dimensional accuracy but inadequate surface properties due to the layer manufacturing process that always entails the use of post-processing techniques to improve the surface quality, increasing the lead times of the whole process despite the reduction of the supply chain. The goal of this work was to provide a comparison between Ti6Al4V samples fabricated by selective laser melting (SLM) and electron beam melting (EBM) with different building directions in relation to the building plate. The results highlighted the influence of the process technique on osteoblast attachment and mineralization compared with the building orientation that showed a limited effect in promoting a proper osseointegration over a long-term period.

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“…However, low osteoconduction and integration of titanium-based implants with the bone for long-term survival, their weak anti-inflammatory properties, and the possibility of toxic components releasing into the human body requires surface modification and the formation of a layer, which significantly eliminates these above-mentioned adverse factors. These surface modifications can be carried out into two ways: (a) The roughness and wettability changes of the titanium implants' surface, which can stimulate a durable connection between the implant and the bone [9][10][11]; and (b) the formation of bioactive coatings, which accelerate bone formation (e.g., hydroxyapatite layers [12,13]) or increase their biocidal activity (e.g., bio-functional magnesium coating, as well as silver nanoparticles [14][15][16]). The formation of an oxide layer (passivation layer) on the surface of titanium/titanium alloy implants, which is practically insoluble and largely responsible for their high corrosion resistance and biocompatibility, is an important way to approach implants' surface modification [17].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Evaluation of the Biological Properties of Surface-Modified Titanium Alloy Implants

Piszczek

Radtke

Ehlert

et al. 2020

JCM

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An increasing interest in the fabrication of implants made of titanium and its alloys results from their capacity to be integrated into the bone system. This integration is facilitated by different modifications of the implant surface. Here, we assessed the bioactivity of amorphous titania nanoporous and nanotubular coatings (TNTs), produced by electrochemical oxidation of Ti6Al4V orthopedic implants' surface. The chemical composition and microstructure of TNT layers was analyzed by X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD). To increase their antimicrobial activity, TNT coatings were enriched with silver nanoparticles (AgNPs) with the chemical vapor deposition (CVD) method and tested against various bacterial and fungal strains for their ability to form a biofilm. The biointegrity and anti-inflammatory properties of these layers were assessed with the use of fibroblast, osteoblast, and macrophage cell lines. To assess and exclude potential genotoxicity issues of the fabricated systems, a mutation reversal test was performed (Ames Assay MPF, OECD TG 471), showing that none of the TNT coatings released mutagenic substances in long-term incubation experiments. The thorough analysis performed in this study indicates that the TNT5 and TNT5/AgNPs coatings (TNT5-the layer obtained upon applying a 5 V potential) present the most suitable physicochemical and biological properties for their potential use in the fabrication of implants for orthopedics. For this reason, their mechanical properties were measured to obtain full system characteristics.

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Rational design of additively manufactured Ti6Al4V implants to control Staphylococcus aureus biofilm formation

Cited by 52 publications

References 57 publications

Bacterial adhesion to biomaterials: What regulates this attachment? A review

Bacterial adhesion to biomaterials: What regulates this attachment? A review

Selective Laser Melting and Electron Beam Melting of Ti6Al4V for Orthopedic Applications: A Comparative Study on the Applied Building Direction

Comprehensive Evaluation of the Biological Properties of Surface-Modified Titanium Alloy Implants

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