Rational design of protamine nanocapsules as antigen delivery carriers

González-Aramúndiz, José Vicente; Presas, Elena; Dalmau-Mena, Inmaculada; Martínez-Pulgarín, Susana; Alonso, Covadonga López; Escribano, José M.; Alonso, María José; Csaba, Nœmi

doi:10.1016/j.jconrel.2016.11.012

Cited by 47 publications

(48 citation statements)

References 32 publications

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“…Protamine content was analyzed by HPLC; the interaction of the polypeptide with SLN surface was also high, with 64 ± 4% of the initial protamine detected after TFF washing step, which is in agreement with data reported for other lipid nanocarriers containing PEG stearate [34]. (Figure 1).…”

Section: Sln Preparation and Characterizationsupporting

confidence: 89%

Solid lipid nanoparticles for the delivery of anti-microbial oligonucleotides

González-Paredes¹,

Sitia

Ruyra

et al. 2019

European Journal of Pharmaceutics and Biopharmaceutics

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Novel alternatives to antibiotics are urgently needed for the successful treatment of antimicrobial resistant (AMR) infections. Experimental antibacterial oligonucleotide therapeutics, such as transcription factor decoys (TFD), are a promising approach to circumvent AMR. However, the therapeutic potential of TFD is contingent upon the development of carriers that afford efficient DNA protection against nucleases and delivery of DNA to the target infection site. As a carrier for TFD, here we present three prototypes of anionic solid lipid nanoparticles that were coated with either the cationic bolaamphiphile 12-bistetrahydroacridinium or with protamine. Both compounds switched particles zeta potential to positive values, showing efficient complexation with TFD and demonstrable protection from deoxyribonuclease. The effective delivery of TFD into bacteria was confirmed by confocal microscopy while SLN-bacteria interactions were studied by flow cytometry. Antibacterial efficacy was confirmed using a model TFD

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Section: Sln Preparation and Characterizationsupporting

confidence: 89%

Solid lipid nanoparticles for the delivery of anti-microbial oligonucleotides

González-Paredes¹,

Sitia

Ruyra

et al. 2019

European Journal of Pharmaceutics and Biopharmaceutics

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“…Solvent displacement technique was used for the design of CSNCs [ 24 ] and the rational design was based on obtaining the smallest size of particles possible that also had an adequate zeta potential. We used an experimental design for the development of a primary nanoemulsion (NE) in order to achieve this.…”

Section: Resultsmentioning

confidence: 99%

“…NE were prepared using the solvent displacement technique [ 24 ]. Briefly, different amounts of LP-HCO, TCPH, and EmFAS-30 were dissolved in 750 µL ethanol followed by the addition of 4.25 mL of acetone.…”

Section: Methodsmentioning

confidence: 99%

Lower-Sized Chitosan Nanocapsules for Transcutaneous Antigen Delivery

2018

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Transcutaneous vaccination has several advantages including having a noninvasive route and needle-free administration; nonetheless developing an effective transdermal formulation has not been an easy task because skin physiology, particularly the stratum corneum, does not allow antigen penetration. Size is a crucial parameter for successful active molecule administration through the skin. Here we report a new core-shell structure rationally developed for transcutaneous antigen delivery. The resulting multifunctional carrier has an oily core with immune adjuvant properties and a polymeric corona made of chitosan. This system has a size of around 100 nm and a positive zeta potential. The new formulation is stable in storage and physiological conditions. Ovalbumin (OVA) was used as the antigen model and the developed nanocapsules show high association efficiency (75%). Chitosan nanocapsules have high interaction with the immune system which was demonstrated by complement activation and also did not affect cell viability in the macrophage cell line. Finally, ex vivo studies using a pig skin model show that OVA associated to the chitosan nanocapsules developed in this study penetrated and were retained better than OVA in solution. Thus, the physicochemical properties and their adequate characteristics make this carrier an excellent platform for transcutaneous antigen delivery.

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“…In addition, an important accumulation of the drugs in the lymphatic system was observed. In the vaccinology field, we have successfully associated different types of protein antigens, such as tetanus toxoid, influenza antigen, recombinant hepatitis B surface antigen, and IutA E. coli antigen to different NCs. The overall result observed, when using these antigens, was an increased immunogenic response following either, intramuscular or intranasal immunization.…”

Section: Introductionmentioning

confidence: 99%

Engineering, on‐demand manufacturing, and scaling‐up of polymeric nanocapsules

Crecente‐Campo

Alonso

2018

Bioengineering & Transla Med

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Polymeric nanocapsules are versatile delivery systems with the capacity to load lipophilic drugs in their oily nucleus and hydrophilic drugs in their polymeric shell. The objective of this work was to expand the technological possibilities to prepare customized nanocapsules. First, we adapted the solvent displacement technique to modulate the particle size of the resulting nanocapsules in the 50–500 nm range. We also produced nanosystems with a shell made of one or multiple polymer layers i.e. chitosan, dextran sulphate, hyaluronate, chondroitin sulphate, and alginate. In addition, we identified the conditions to translate the process into a miniaturized high‐throughput tailor‐made fabrication that enables massive screening of formulations. Finally, the production of the nanocapsules was scaled‐up both in a batch production, and also using microfluidics. The versatility of the properties of these nanocapsules and their fabrication technologies is expected to propel their advance from bench to clinic.

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Rational design of protamine nanocapsules as antigen delivery carriers

Cited by 47 publications

References 32 publications

Solid lipid nanoparticles for the delivery of anti-microbial oligonucleotides

Solid lipid nanoparticles for the delivery of anti-microbial oligonucleotides

Lower-Sized Chitosan Nanocapsules for Transcutaneous Antigen Delivery

Engineering, on‐demand manufacturing, and scaling‐up of polymeric nanocapsules

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