rDNA sequence analysis confirms the distinction between Colletotrichum graminicola and C. sublineolum

Sherriff, Christine; Whelan, M.J.; Arnold, G. M.; Bailey, J. A.

doi:10.1016/s0953-7562(09)80649-7

Cited by 90 publications

(43 citation statements)

References 9 publications

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“…However, our studies showed that the divergence of 5.8S-ITS region sequences among isolates of C. fragariae and C. gloeosporioides was too low to separate them confidently as different species, confirming the observations of Sherriff et al (1995), 500 bp…”

Section: Discussionsupporting

confidence: 79%

Identification of Colletotrichum spp. isolated from strawberry in Zhejiang Province and Shanghai City, China

Xie

Zhang

Wan

et al. 2010

J. Zhejiang Univ. Sci. B

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Strawberry anthracnose, caused by Colletotrichum spp., is a major disease of cultivated strawberry. This study identifies 31 isolates of Colletotrichum spp. which cause strawberry anthracnose in Zhejiang Province and Shanghai City, China. Eleven isolates were identified as C. acutatum, 10 as C. gloeosporioides and 10 as C. fragariae based on morphological characteristics, phylogenetic and sequence analyses. Species-specific polymerase chain reaction (PCR) and enzyme digestion further confirmed the identification of the Colletotrichum spp., demonstrating that these three species are currently the causal agents of strawberry anthracnose in the studied regions. Based on analysis of rDNA internal transcribed spacers (ITS) sequences, sequences of all C. acutatum were identical, and little genetic variability was observed between C. fragariae and C. gloeosporioides. However, the conservative nature of the MvnI specific site from isolates of C. gloeosporioides was confirmed, and this site could be used to differentiate C. gloeosporioides from C. fragariae.

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Section: Discussionsupporting

confidence: 79%

Identification of Colletotrichum spp. isolated from strawberry in Zhejiang Province and Shanghai City, China

Xie

Zhang

Wan

et al. 2010

J. Zhejiang Univ. Sci. B

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“…This study clearly confirmed that strains of C. graminicola infecting sorghum did not infect millet and were different from strains infecting maize, particularly in Nigeria and possibly in the whole of West and Central Africa. This supports the designation of strains infecting sorghum as C. sublineolum on the basis of their appressorial morphology while strains infecting maize were designated C. graminicola (Sutton, 1968;Sutton, 1992) and rDNA analysis Sheriff et al, 1995).…”

Section: The Pathogen Etiology and Symptomatologysupporting

confidence: 68%

Sorghum Anthracnose and Sustainable Management Strategies in West and Central Africa

Marley

Diourté

Néya

et al. 2005

Journal of Sustainable Agriculture

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ABSTRACT. Anthracnose caused by Colletotrichum graminicola remains the most important foliar disease of sorghum in West and Central Africa. This paper describes the advances made in sorghum anthracnose research that has led to a better understanding of pathogenic and genotypic diversity, epidemiology and important disease management strategies. We further highlight how understanding pathogen diversity interplays with the major sustainable anthracnose management strategies such as the use of host plant resistance and crop residue management within the region.

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“…Isolates 1, 2 and 3 originated from the same locality, which demonstrates the occurrence of genetic variability in the C. lindemuthianum fungus. Sherriff et al (1994) and Sherriff, Whelan, Arnold, and Bailey (1995) obtained similar results for the ITS2 region when they compared Colletotrichum species. …”

Section: Dna Ext Regionsmentioning

confidence: 55%

<b>Characterization of race 65 of <i>Colletotrichum lindemuthianum</i> by sequencing ITS regions

et al. 2016

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ABSTRACT. The present work aimed characterize isolates of C. lindemuthianum race 65 from different regions in Brazil by ITS sequencing. A total of 17 isolates of race 65, collected in the states of Mato Grosso, Minas Gerais, Paraná, Santa Catarina and São Paulo, were studied. Analysis of the sequences of isolates 8, 9, 12, 14 and 15 revealed the presence of two single nucleotide polymorphisms (SNPs) in the ITS1 region at the same positions. These isolates, when analyzed together with the sequence of isolate 17, revealed a SNP in the ITS2 region. The highest genetic dissimilarity, observed between isolates 11 and 3 and between isolates 11 and 10, was 0.772. In turn, isolates 7 and 2 were the most similar, with a value of 0.002 for genetic distance. The phylogenetic tree obtained based on the sequences of the ITS1 and ITS2 regions revealed the formation of two groups, one with a subgroup. The results reveal high molecular variability among isolates of race 65 of C. lindemuthianum.Keywords: anthracnose, genetic variability, pathogens, dissimilarity.Caracterização da raça 65 de Colletotrichum lindemuthianum utilizando sequenciamento das regiões ITS RESUMO. O presente trabalho teve como objetivo caracterizar isolados de C. lindemuthianum da raça 65 provenientes de diversas regiões do Brasil, por meio de sequenciamento de regiões ITS. Um total de 17 isolados da raça 65, coletados nos estados do Mato Grosso, Minas Gerais, Paraná, Santa Catarina e São Paulo, foram estudados. As análises das sequências dos isolados 8, 9, 12, 14 e 15 revelaram a presença de dois SNPs na região ITS1 nas mesmas posições. Estes mesmos isolados quando analisados juntamente com a sequência do isolado 17 apresentaram um SNP na região ITS2. A maior dissimilaridade genética foi de 0,772 observada entre os isolados 11 e 3 e entre os isolados 11 e 10. Por sua vez, os isolados 7 e 2 foram os mais similares, com valor de distância genética de 0,002. A árvore filogenética obtida com base nas sequências das regiões ITS1 e ITS2 revelou a formação de dois grupos, sendo um com a divisão de um subgrupo. Estes resultados revelam uma elevada variabilidade molecular entre isolados da raça 65 de C. lindemuthianum.

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rDNA sequence analysis confirms the distinction between Colletotrichum graminicola and C. sublineolum

Cited by 90 publications

References 9 publications

Identification of Colletotrichum spp. isolated from strawberry in Zhejiang Province and Shanghai City, China

Identification of Colletotrichum spp. isolated from strawberry in Zhejiang Province and Shanghai City, China

Sorghum Anthracnose and Sustainable Management Strategies in West and Central Africa

<b>Characterization of race 65 of <i>Colletotrichum lindemuthianum</i> by sequencing ITS regions

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